Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (26): 4118-4122.doi: 10.12307/2022.812
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Xu Yuan1, Niu Yulin1, Yuan Zhihui1, Jia Lei2, Pan Guanghui1
Received:
2021-07-05
Accepted:
2021-08-21
Online:
2022-09-18
Published:
2022-03-08
Contact:
Pan Guanghui, Chief physician, Department of Organ Transplantation, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
About author:
Xu Yuan, Master, Associate chief physician, Department of Organ Transplantation, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
Supported by:
CLC Number:
Xu Yuan, Niu Yulin, Yuan Zhihui, Jia Lei, Pan Guanghui. Up-regulating follicular regulatory T cells for treating antibody-mediated rejection after kidney transplantation[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(26): 4118-4122.
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苏木精-伊红染色显示,排斥反应组患者肾组织内的炎细胞浸润数量多于免疫耐受组[(87.17±13.56),(18.11±3.95)个/视野,t=4.885,P < 0.001];免疫组化染色显示,排斥反应组患者肾组织内可见广泛的C4d阳性表达,免疫耐受组未见检测到C4d表达。 2.2 两组患者外周血淋巴细胞内的miR-4286 mRNA表达 RT-PCR检测结果显示,排斥反应组与免疫耐受组患者外周血淋巴细胞中的miR-4286 mRNA表达量分别为3.95±0.32,2.18±0.27,组间比较差异有显著性意义(t=4.331,P=0.002)。 2.3 两组患者外周血淋巴细胞中Tfh与Tfr细胞的比例 两组患者外周血淋巴细胞流式细胞术检测结果,见图2。"
与对照组比较,miR-4286过表达组的转化生长因子β水平降低(t=4.846,P=0.003),干扰素γ、白细胞介素6、白细胞介素21水平升高(t=4.304,P=0.005;t=3.844,P=0.003;t=7.600,P < 0.001);miR-4286沉默组转化生长因子β水平升高(t=15.053,P < 0.001),干扰素γ、白细胞介素6、白细胞介素21水平降低(t=2.929,P=0.026;t=3.563,P=0.005;t=5.439,P < 0.001)。与miR-4286沉默组比较,转化生长因子β阻断组转化生长因子β水平降低(t=1.227,P < 0.001),干扰素γ、白细胞介素6、白细胞介素21水平升高(t=1.001,P < 0.05;t=2.441,P=0.005;t=1.554,P < 0.001)。 2.4.2 各组细胞中B细胞和浆细胞的比例 各组淋巴细胞B细胞和浆细胞比例流式细胞术检测结果,见图3。"
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