Expression of vasohibin-1 and other factors after the co-culture of two kinds of Echinococcus granulosus protoscolex and endothelial progenitor cells

doi:10.12307/2022.572

Abstract

Abstract: BACKGROUND: Alveolar echinococcosis and cystic echinococcosis grow in different ways in the body. Alveolar echinococcosis is infiltrating growth, which can invade tissues, blood vessels, and bile ducts. Cystic echinococcosis has fibrous outer cyst wall-wrapped lesions, which show compressive growth of tissues. The effect of the two types of hydatidosis on the invasion and formation of blood vessels is still unclear.
OBJECTIVE: To explore the effects of two kinds of Echinococcus granulosus protoscolex on angiogenesis-related factors in endothelial progenitor cells.
METHODS: After extraction, culture, and identification of endothelial progenitor cells from C57BL/6 mouse bone marrow, and extraction of two types of Echinococcus granulosus protoscolex, two kinds of Echinococcus granulosus protoscolex were co-cultured with endothelial progenitor cells for 24, 48, and 60 hours. ELISA was applied to detect the secretion of vascular endothelial growth factor A and stromal cell-derived factor-1α in the supernatant of cell co-culture. Western blot assay was utilized to detect the relative expression of vasohibin-1, vascular endothelial growth factor A, hypoxia-inducible factor 1α, and stromal cell-derived factor-1α protein in endothelial progenitor cells of each group.
RESULTS AND CONCLUSION: (1) The levels of vascular endothelial growth factor A and stromal cell-derived factor-1α were significantly higher in the alveolar echinococcosis group than those in the cystic echinococcosis group at 60 hours of coculture (P < 0.05). (2) Relative expression levels of vasohibin-1, hypoxia-inducible factor 1α, and stromal cell-derived factor-1α were higher in the alveolar echinococcosis group than those in the cystic echinococcosis group at 48 and 60 hours of coculture (P < 0.05). (3) The relative expression levels of vascular endothelial growth factor A were not significantly different in the alveolar echinococcosis and cystic echinococcosis groups at 48 and 60 hours of coculture (P > 0.05). (4) It is concluded that alveolar echinococcosis promotes angiogenesis, and cystic echinococcosis has little effect on angiogenesis.

Key words: endothelial progenitor cells, alveolar echinococcosis, cystic echinococcosis, vasohibin-1, vascular endothelial growth factor, hypoxia-inducible factor 1α

CLC Number:

Yu Xiaofan, Jiang Huijiao, Tan Xiaowu, Wu Xiangwei. Expression of vasohibin-1 and other factors after the co-culture of two kinds of Echinococcus granulosus protoscolex and endothelial progenitor cells[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(24): 3892-3896.

Figures/Tables 4

References

[1] 沈舒.泡型肝包虫病诊疗专家共识(2020版)[J].中国普外基础与临床杂志, 2020,27(1):13-17.
[2] 苟平,王志鑫,胡陈亮,等.中国西部地区泡型、囊型包虫病手术治疗方式与术后并发症的回顾性分析[J].实用临床医药杂志,2019,23(3):33-37.
[3] 郭亚民,朱文君,赵顺云,等.复杂性肝棘球蚴病外科治疗策略研究进展[J].中国血吸虫病防治杂志,2018,30(6):705-708.
[4] 周启锋,任利,张灵强,等.肝泡状棘球蚴病动物模型的研究进展[J].临床肝胆病杂志,2017,33(11):2247-2250.
[5] 尼顿,米玛. 52例肝包囊虫病CT表现回顾性分析[J].西藏医药,2018,39(5): 64-65.
[6] 曾辉,刘明琪,泽仁措.肝囊型包虫病的超声图像分析[J].中国城乡企业卫生,2017,32(6):94-96.
[7] 张皓,李豪胜,杨茂江,等.肝细粒棘球蚴病的影像病理学对比分析[J].中国人兽共患病学报,2017,33(9):845-847.
[8] 黄玉洁,赵建卿,王健,等.多层螺旋CT及血管成像对肝泡状棘球蚴血管受侵的诊断价值[J].新疆医科大学学报,2015,38(10):1216-1219.
[9] 蒲鹏,代静.多层螺旋CT在脊椎泡状棘球蚴病中的诊断价值[J].中国现代医生,2019,57(5):103-105.
[10] 徐睿,余鑫.肝泡型包虫病CT钙化特点及其临床应用价值[J].贵州医药, 2019,43(9):1459-1461.
[11] 赵小峰.人VASH_1蛋白结构和功能的生物信息学分析[J].生物技术,2015, 25(6):552-557.
[12] 高静,李晓岚,王敏哲,等.血管内皮生长因子、基质细胞衍生因子-1基因对人微血管内皮细胞增殖、迁移、凋亡的影响[J].安徽医药,2019,23(9): 1784-1789.
[13] 韩晓宇,师永红.低氧诱导因子-1α参与肿瘤血管形成的生物学机制研究进展[J].疾病监测与控制,2021,15(1):77-80.
[14] 杨锦锋,曾荣耀.血管内皮生长因子和缺氧诱导因子-1α的表达与原发性肝癌患者临床病理特征及预后的关系[J].中国慢性病预防与控制,2020, 28(12):924-927.
[15] 王威,张颖倩,李中轩,等.内皮祖细胞促进损伤血管再内皮化的机制[J].临床心血管病杂志,2019,35(8):763-767.
[16] 毛梅,王健瑜,汪丽,等.内皮祖细胞对急性损伤肺组织的抗炎作用[J].第三军医大学学报,2015,37(24):2444-2447.
[17] WANG X, ZHAO Z, ZHANG H, et al. Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow. Exp Ther Med. 2018;16(6):5171-5177.
[18] 桂显伟,姜慧娇,武杰,等.两种棘球绦虫原头节促进骨髓间充质干细胞钙化的差异性分析[J].中国组织工程研究,2021,25(1):38-43.
[19] 赵兴华.对肝泡状棘球蚴病患者CT图像特征的分析[J].当代医药论丛,2017, 15(3):133-135.
[20] 尹建海,沈玉娟,于爱萍,等.小鼠细粒棘球蚴体外促血管生成作用研究[J].中国血吸虫病防治杂志,2017,29(3):320-323.
[21] 郭黎姣,姜慧娇,韩欢欢,等.肝泡状棘球蚴组织中HIF-1α、VEGFA的表达及对血管生成的作用[J].中国人兽共患病学报,2019,35(7):639-646.
[22] 曾红春,王颖鑫,王俊华,等.CEUS评价大鼠肝泡状棘球蚴病灶血流灌注的动态演变[J].中国介入影像与治疗学,2017,14(9):566-570.
[23] 陈曦,张先兵,李勋,等.内皮祖细胞修复血管损伤的研究[J].中国现代普通外科进展,2015,18(11):908-911.
[24] HAN X, JIANG G, SHI Q. Effects of antihyperglycemics on endothelial progenitor cells. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2020;49(5):629-636.
[25] 林上进,程群.内皮祖细胞与骨折愈合[J].国际骨科学杂志,2016,37(1):31-35.
[26] WATANABE T, HOSAKA T, OHMORI-MATSUDA K, et al. High preoperative plasma vasohibin-1 concentration predicts better prognosis in patients with non-small cell lung carcinoma. Health Sci Rep. 2018;1(6):e40.
[27] NINOMIYA Y, OZAWA S, OGUMA J, et al. Expression of vasohibin-1 and -2 predicts poor prognosis among patients with squamous cell carcinoma of the esophagus. Oncol Lett. 2018;16(4):5265-5274.
[28] MIKAMI S, OYA M, KOSAKA T, et al. Increased vasohibin-1 expression is associated with metastasis and poor prognosis of renal cell carcinoma patients. Lab Invest. 2017;97(7):854-862.
[29] SAITO M, SUZUKI Y, YANO S, et al. Proteolytic inactivation of anti-angiogenic vasohibin-1 by cancer cells. J Biochem. 2016;160(4):227-232.
[30] 曹宸,辜敏,刘文能.内皮祖细胞研究进展及其在肿瘤治疗中的潜在应用[J].西南国防医药,2019,29(4):508-510.