Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (34): 5413-5419.doi: 10.12307/2021.232
Xiao Deli1, Sun Yinzhe1, Cui Cheng1, Liu Bo2
Received:
2020-05-25
Revised:
2020-05-26
Accepted:
2020-09-21
Online:
2021-12-08
Published:
2021-07-26
Contact:
Xiao Deli, PhD, Associate professor, School of Science, China Pharmaceutical University, Nanjing 211198, Jiangsu Province, China
About author:
Xiao Deli, PhD, Associate professor, School of Science, China Pharmaceutical University, Nanjing 211198, Jiangsu Province, China
Supported by:
CLC Number:
Xiao Deli, Sun Yinzhe, Cui Cheng, Liu Bo. Preparation and degradation properties of concentrated growth factor fibrin membrane[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(34): 5413-5419.
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2 结果与分析 Results and analysis 2.1 两种纤维蛋白膜的体外降解性能差异 2.1.1 两种纤维蛋白膜的质量变化 见图3A。 重复测量方差分析组内比较结果显示,随着时间的变化,富血小板纤维蛋白组(F=17 793.560,P < 0.05)与浓缩生长因子纤维蛋白组(F=49 986.785,P < 0.05)中纤维蛋白膜的质量都发生了明显变化。 组内进一步方差分析比较结果显示,富血小板纤维蛋白膜的质量在实验前9 d均表现为显著下降(P < 0.05),从第9天开始质量残余量很少,可认为降解完全;浓缩生长因子纤维蛋白膜的质量除第5,10天外在实验前14 d均表现为显著下降(P < 0.05),从第14天开始质量残余量很少,可认为降解完全。 组间进一步t检验比较结果显示,实验开始后,两种纤维蛋白膜的质量在各时间点均有显著差异(P < 0.05)。 2.1.2 两种纤维蛋白膜的面积变化 见图3B。 重复测量方差分析组内比较结果显示,随着时间的变化,富血小板纤维蛋白组(F=14 124.200,P < 0.05)与浓缩生长因子纤维蛋白组(F=48 061.405,P < 0.05)中纤维蛋白膜的面积都发生了明显变化。 组内进一步方差分析比较结果显示,富血小板纤维蛋白膜的面积除第2天到第3天、第4天到第5天变化比较平缓外,在实验前9 d均表现为显著下降(P < 0.05),从第9天开始面积残余量很少,可认为降解完全;浓缩生长因子纤维蛋白膜的面积除第4天到第5天变化比较平缓,在实验前14 d均表现为显著下降(P < 0.05),从第14天开始面积残余量很少,可认为降解完全。 组间进一步t检验比较结果显示,实验开始后,两种纤维蛋白膜的面积在各时间点均有显著差异(P < 0.05)。 "
2.1.3 两种纤维蛋白膜的降解性能 真空采血管中的血液样本分别运行富血小板纤维蛋白和浓缩生长因子纤维蛋白程序后,均被分成3层,由底端到上端依次为红细胞层、浓缩生长因子纤维蛋白层和血浆层。浓缩生长因子纤维蛋白膜的初始质量与富血小板纤维蛋白膜的初始质量存在差异,浓缩生长因子纤维蛋白膜的初始质量大于富血小板纤维蛋白膜,这说明浓缩生长因子纤维蛋白膜比富血小板纤维蛋白膜有更多的纤维蛋白和生长因子。其次,浓缩生长因子纤维蛋白膜的质量和膜面积变化较富血小板纤维蛋白膜稳定。在降解过程刚开始的几天里,浓缩生长因子纤维蛋白相较于富血小板纤维蛋白降解速率更慢,能够更长时间地保持初始的质量和面积。最后,浓缩生长因子纤维蛋白膜的降解时间比富血小板纤维蛋白膜要长,有更稳定的控释生长因子能力。因此与富血小板纤维蛋白膜相比,浓缩生长因子纤维蛋白膜在医疗领域有望展现更优异的性能[33]。两种纤维蛋白膜的体外降解过程见图4。 "
如图5所示,两组浓缩生长因子纤维蛋白膜总体降解趋势相似。经t检验分析,同种型号不同机器制备的浓缩生长因子纤维蛋白膜的初始质量无差异(P > 0.05),说明同种型号不同机器制备浓缩生长因子纤维蛋白凝胶具有较好的重现性。但在人工压制成膜的过程中,不同批次间得到的初始膜面积会存在差异。为便于解释,作者提出了初始面积质量比(k)的概念:初始质量(m0)相同的浓缩生长因子纤维蛋白凝胶,初始膜面积(S0)越小则初始面积质量比越小,理论上降解速度也越慢。 研究发现,组二的初始面积质量比较小,降解速度因此较慢,与理论相吻合。迄今为止,国内外尚无关于浓缩生长因子纤维蛋白膜初始面积质量比的论述。其意义在于:在临床应用时,医生希望浓缩生长因子纤维蛋白膜有合适的降解时间,实验揭示了可以通过改变浓缩生长因子纤维蛋白膜的初始面积质量比来灵活调控其降解时间。"
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