Low-intensity pulsed ultrasound mediates bone marrow mesenchymal stem cells to promote osteoarthritis cartilage repair

doi:10.12307/2021.135

Abstract

Abstract: BACKGROUND: Studies have shown that bone marrow mesenchymal stem cells and low-intensity pulsed ultrasound can promote the repair of knee osteoarthritis cartilage, but there is no report whether low-intensity pulsed ultrasound-mediated bone marrow mesenchymal stem cells can have a better effect on knee osteoarthritis cartilage repair.

OBJECTIVE: To observe the repair effect of bone marrow mesenchymal stem cells mediated by low-intensity pulsed ultrasound on knee osteoarthritis cartilage.

METHODS: Totally 36 Sprague-Dawley male rats aged 6 weeks were randomly divided into four groups: control group, osteoarthritis group, stem cell group and stem cell plus ultrasound group (n=9 per group). In the osteoarthritis group, stem cell group and stem cell plus ultrasound group, osteoarthritis models were made by the anterior cruciate ligament transection of the right knee joint with partial resection of the medial meniscus. At 6 weeks after model establishment, no special treatment was given to the control group and the osteoarthritis group. In the stem cell group and the stem cell plus ultrasound group, the knee joint cavity was injected with bone marrow mesenchymal stem cells 0.1 mL (cell concentration was 1×109 L-1), once a week, for 4 weeks. Simultaneously, rats in the stem cells plus ultrasound group were treated with low-intensity pulsed ultrasound at 20 minutes/day for 4 weeks. Western blot assay was used to detect the expression of type II collagen and Sox9 protein in rat articular cartilage, and Mankin score was used to evaluate the degree of cartilage degeneration in each group of experimental rats.
RESULTS AND CONCLUSION: (1) After 4 weeks of treatment, the expression of type II collagen and Sox9 protein in the osteoarthritis group was reduced compared to the other three groups (P < 0.05). The protein expression of type II collagen and Sox9 in the stem cell group was lower than that of stem cells plus ultrasound group (P < 0.05). (2) The Mankin scores of control group, stem cell group and stem cell plus ultrasound group were significantly lower than those of the osteoarthritis group (P < 0.01). Compared with the stem cell group, the Mankin score of the stem cell plus ultrasound group was lower (P < 0.05). (3) The results show that compared with simple bone marrow mesenchymal stem cells, low-intensity pulsed ultrasound-mediated bone marrow mesenchymal stem cells have a better effect on cartilage repair in knee osteoarthritis.

Key words: stem cells, bone marrow mesenchymal stem cells, low-intensity pulsed ultrasound, osteoarthritis, knee, cartilage, repair

CLC Number:

Liao Qing, Li Baojian, Li Yang, Xu Taixiang, Zeng Jing, Zhang Zhenzhen, Liu Gang. Low-intensity pulsed ultrasound mediates bone marrow mesenchymal stem cells to promote osteoarthritis cartilage repair[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(31): 4950-4955.

Figures/Tables 6

References

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