中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (51): 9616-9623.doi: 10.3969/j.issn.2095-4344.2012.51.022

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

丝素蛋白材料复合骨髓间充质干细胞修复皮肤创面

苗宗宁1,2，李 芳2，张学光2，吕国忠1   

  • 出版日期:2012-12-16 发布日期:2012-12-16

Repairing full-thickness cutaneous defects with silk fibrin combined with bone marrowmesenchymal stem cells

Miao Zong-ning1, 2, Li Fang2, Zhang Xue-guang2, Lü Guo-zhong1   

  • Online:2012-12-16 Published:2012-12-16

PDF

900

被引次数

19

摘要：

背景：丝素蛋白材料具有良好的生物相容性。
目的：观察丝素蛋白材料复合骨髓间充质干细胞修复大鼠全层皮肤缺损的可行性。
方法：应用5-溴脱氧尿核苷标记SD大鼠骨髓间充质干细胞，并复合丝素蛋白材料培养。建立SD大鼠全层皮肤缺损模型，随机分组：实验组移植同种异体骨髓间充质干细胞与丝素蛋白材料复合物，细胞组移植骨髓间充质干细胞，对照组移植丝素蛋白材料，空白组不作处理。
结果与结论：①大体观察：空白组术后8 周仍可见坏死组织，且瘢痕挛缩明显；细胞组与空白组愈合情况相近。对照组术后8周未愈合，有少量瘢痕形成，与周围皮肤融为一体。实验组术后4周创面无明显瘢痕形成，术后8周愈合良好。②组织学观察：实验组术后4周免疫荧光染色显示带有5-溴脱氧尿核苷标记的骨髓间充质干细胞定位在重建的表皮和真皮组织中，术后8 周创面愈合良好；细胞组免疫荧光染色显示少量带有5-溴脱氧尿核苷标记的骨髓间充质干细胞存在。表明丝素蛋白材料与骨髓间充质干细胞联合移植可修复大鼠全层皮肤缺损。

Abstract:

BACKGROUND: The silk fibroin material has good biocompatibility.
OBJECTIVE: To observe the feasibility of silk fibroin material and bone marrow mesenchymal stem cells for repairing the rat full-thickness cutaneous defects.
METHODS: The bone marrow mesenchymal stem cells of Sprague-Dawley rats were labeled with 5-bromodeoxyuridine and cultured with silk fibroin material. The Sprague-Dawley rat full-thickness cutaneous defect models were prepared and divided into four groups: the experimental group was transplanted with the complex of allogeneic bone marrow mesenchymal stem cells and silk fibroin material; the cell group was transplanted with bone marrow mesenchymal stem cells; the control group was transplanted with silk fibroin material; and the blank group without any treatment.
RESULTS AND CONCLUSION: ①General observation: the necrotic tissue was observed in the blank group at 8 weeks after operation and the scar contracture was obvious; the wound healing in the cell group was similar with that in the blank group. The wound in the control group was not healed and a little amount of scar was observed and integrated with the surrounding skin. There was no obvious scar in the experimental group at 4 weeks after operation and the wound was healed well at 8 weeks after operation. ②Histological observation: the immunofluorescence staining in the experimental group at 4 weeks after operation showed that the 5-bromodeoxyuridine labeled bone marrow mesenchymal stem cells were located in reconstructed epidermis and dermal tissues, and wound surface was recovered well at 8 weeks after operation; the immunofluorescence staining in the cell group showed a little amount of 5-bromodeoxyuridine labeled bone marrow mesenchymal stem cells. It indicates that co-transplantation of silk fibroin material and bone marrow mesenchymal stem cells can repair rat full-thickness cutaneous defects.