中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (50): 9414-9418.doi: 10.3969/j.issn.2095-4344.2012.50.019

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

寒燥对不同致病因素诱发模型骨组织基质金属蛋白酶1 mRNA表达的影响

李风森1，高 振1, 2，徐 丹1，荆 晶1，王 晶1   

  • 出版日期:2012-12-09 发布日期:2012-12-09

Effect of cold and dry environment on the expression of matrix metalloproteinase 1 mRNA in bone tissues of different pathogenic factors induced models

Li Feng-sen1, Gao Zhen1, 2, Xu Dan1, Jing Jing1, Wang Jing1   

  • Online:2012-12-09 Published:2012-12-09

PDF

535

被引次数

1

摘要：

背景：研究发现新疆干燥寒冷的环境对当地多种疾病的发生发展产生影响，但目前具体机制不明，不利于疾病地域化、个性化治疗的开展。
目的：揭示寒燥环境复合不同的疾病诱发因素对大鼠骨组织基质金属蛋白酶1mRNA表达的影响。
方法：利用寒燥环境分别与熏烟、熏烟加气道滴注胰蛋白酶复合作用于大鼠建立模型，利用荧光定量PCR法检测骨组织中基质金属蛋白酶1 mRNA的表达。
结果与结论：寒燥+熏烟组骨组织中基质金属蛋白酶1 mRNA表达高于熏烟组(P < 0.05)；寒燥+熏烟+胰酶组骨组织中基质金属蛋白酶1 mRNA表达高于寒燥+胰酶组(P < 0.01)。提示寒燥可以加剧熏烟、熏烟加胰酶等不同疾病诱发因素对骨组织的损伤程度。

Abstract:

BACKGROUND: The studies have showed that the dry and cold environment of Xinjiang area can affect many diseases, but the specific mechanism is unknown, which is not conducive to carry out the geographical and personalized treatment for the diseases.
OBJECTIVE: To reveal the effect of cold and dry environment composite different diseases predisposing factor on the expression of matrix metalloproteinase 1 mRNA in rat bone tissue.
METHODS: The models were established by smoking or dripping pancreatic elastase in trachea combined with smoking, and stimulated with cold and dry environment, the fluorescence quantitative PCR was used to determine the expression of matrix metalloproteinase 1 mRNA in bone tissue.
RESULTS AND CONCLUSION: The expression of matrix metalloproteinase 1 mRNA in bone tissue of smoking composite dry and cold environment group was higher than that of the smoking group (P < 0.05); the expression of matrix metalloproteinase 1 mRNA in bone tissue of smoking+pancreatic elastase composite dry and cold environment group was higher than that of the smoking+pancreatic elastase group (P < 0.01). It shows that dry and cold environment can exacerbate the damage of bone when compound with different diseases predisposing factors, such as smoking, smoking and pancreatic elastase.