中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (29): 7581-7591.doi: 10.12307/2026.187

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

艾灸干预特应性皮炎模型大鼠作用机制的蛋白组学分析

杨云虹，郭丽花，汤  翰，林绿萍，匡泓俊，赵  宏   

  1. 上海中医药大学深圳医院，广东省深圳市   518001
  • 收稿日期:2025-06-15 修回日期:2025-09-20 出版日期:2026-10-18 发布日期:2026-03-04
  • 通讯作者: 赵宏，博士，主任医师，上海中医药大学深圳医院，广东省深圳市 518001
  • 作者简介:杨云虹，女，1997年生，广西壮族自治区桂林市人，汉族，上海中医药大学在读博士，医师，主要从事针灸防治免疫性疾病研究。
  • 基金资助:
    国家自然科学基金面上项目(82474644)，项目负责人：赵宏；深圳市科技计划资助项目(JCYJ20210324120804012)；项目负责人：赵宏

Proteomic analysis of the mechanism of moxibustion intervention in a rat model of atopic dermatitis 

Yang Yunhong, Guo Lihua, Tang Han, Lin Lvping, Kuang Hongjun, Zhao Hong   

  1. Shenzhen Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shenzhen 518001, Guangdong Province, China
  • Received:2025-06-15 Revised:2025-09-20 Online:2026-10-18 Published:2026-03-04
  • Contact: Zhao Hong, PhD, Chief physician, Shenzhen Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shenzhen 518001, Guangdong Province, China
  • About author:Yang Yunhong, PhD candidate, Physician, Shenzhen Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shenzhen 518001, Guangdong Province, China
  • Supported by:
    the National Natural Science Foundation of China (General Program), No. 82474644 (to ZH); Shenzhen Science and Technology Program, No. JCYJ20210324120804012 (to ZH)

PDF

18

摘要：



文题释义：
蛋白组学：是系统性研究生物体内所有蛋白质的组成、结构、功能及动态变化的学科，核心目标是全面解析特定生理或病理状态下蛋白质的表达水平、相互作用网络及翻译后修饰等分子特征。通过高通量技术结合生物信息学方法，能够定量比较不同样本(如此次研究的正常组和模型组、艾灸组与模型组)的蛋白质表达差异，识别关键调控蛋白及关联信号通路，从而揭示细胞代谢、免疫应答、疾病发生等生命活动的分子机制。
整合素β3：是整合素跨膜受体的β亚基，与α亚基结合后识别细胞外基质，介导细胞黏附及信号转导、调控迁移、增殖等过程。在特应性皮炎中，整合素β3表达上调加剧炎症、屏障破坏及瘙痒。整合素β3作为治疗靶点，抑制其表达可改善皮肤屏障功能并减轻炎症反应。

背景：艾灸治疗特应性皮炎疗效确切，但作用机制有待深入研究。
目的：基于蛋白质组学技术探索艾灸干预特应性皮炎的效应机制。
方法：34只SD大鼠随机分为3组，模型组和艾灸组各12只、空白组10只，前两组采用 2，4-二硝基氯苯诱导特应性皮炎模型，造模成功后，艾灸组予艾柱施灸阿是穴30 min，维持局部温度(43±1) ℃，隔日1次，干预14 d；模型组和空白组予相同时间、强度的抓取及固定。通过湿疹面积及严重度指数评估造模后大鼠皮肤损伤情况；使用基于高效液相色谱-串联质谱联用平台的数据非依赖采集蛋白质组学方法进行大鼠皮肤组织蛋白质组的测定分析；运用MaxQuant、Perseus软件和DAVID(https://david.ncifcrf.gov/)、STRING(https://string-db.org/)等分析工具进行质谱数据解析、蛋白鉴定、差异表达蛋白分析、功能注释及生物信息学分析；采用网络可视化软件Cytoscape 3.9.1构建关联网络图。
结果与结论：①艾灸治疗后的特应性皮炎模型大鼠皮肤损伤评分较模型组明显降低(P < 0.05)，表明造模成功；②艾灸可逆转模型组28个差异表达蛋白的上调和40个差异表达蛋白的下调；③生物信息学分析结果表明，特应性皮炎致病主要涉及的信号通路包括神经活性配体-受体相互作用通路、病毒蛋白与细胞因子及细胞因子受体相互作用通路、炎症介质对瞬时受体电位通道的调节通路、中性粒细胞胞外陷阱形成通路等；其中整合素β3和β-1，4-半乳糖基转移酶蛋白的表达调控可能与特应性皮炎发病机制最相关；④艾灸治疗特应性皮炎的作用机制主要涉及单纯疱疹病毒1型感染、嗅觉转导、甲型流感、类固醇激素生物合成和其他与感染或免疫相关的信号通路等；最相关的蛋白包括核因子κB亚基1、组织相容性复合体蛋白、RT1-Bb、Jak1和Cdk6。提示艾灸对特应性皮炎的干预效应具有多靶点、多途径、多通路的特点，能够逆转特应性皮炎造成的炎症反应，同时能发挥抗炎抗氧化的作用。
https://orcid.org/0000-0002-1629-5179 (杨云虹) 


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 艾灸, 特应性皮炎, 大鼠, 作用机制, 蛋白组学技术, 生物信息学

Abstract: BACKGROUND: Moxibustion has been demonstrated as an effective therapeutic approach for atopic dermatitis, yet its underlying mechanisms remain to be further elucidated.
OBJECTIVE: To investigate the mechanisms of moxibustion intervention in atopic dermatitis using proteomics technology.
METHODS: Thirty-four Sprague-Dawley rats were randomly divided into three groups: a model group (n=12), a moxibustion group (n=12), and a blank group (n=10). The first two groups were induced to develop an atopic dermatitis model using 2,4-dinitrochlorobenzene. Following successful modeling, the moxibustion group received moxibustion therapy with moxa sticks applied to the Ashi point for 30 minutes per session, maintaining a local temperature of 
(43±1) °C, administered every other day over a 14-day intervention period. The model group and blank control group underwent restraint and fixation procedures of the same duration and intensity. Skin lesion severity after modeling was evaluated using the Eczema Area and Severity Index. Proteomic analysis of rat skin tissue was performed using a data-independent acquisition approach on a high-performance liquid chromatography-tandem mass spectrometry platform. Mass spectrometry data processing, protein identification, differential protein expression analysis, functional annotation, and bioinformatics analyses were conducted using MaxQuant, Perseus software, DAVID (https://david.ncifcrf.gov/), and STRING (https://string-db.org/). A correlation network diagram was constructed using the network visualization tool Cytoscape 3.9.1.
RESULTS AND CONCLUSION: (1) The skin lesion score of atopic dermatitis model rats after moxibustion treatment was significantly lower than that in the model group (P < 0.05), indicating successful establishment of the model. (2) Moxibustion was able to reverse the upregulation of 28 differentially expressed proteins and the downregulation of 40 differentially expressed proteins in the model group. (3) Bioinformatics analysis revealed that the main signaling pathways involved in atopic dermatitis pathogenesis included neuroactive ligand-receptor interaction, viral protein-cytokine and cytokine-receptor interaction, inflammatory mediator regulation of transient receptor potential channels, and neutrophil extracellular trap formation. Among these, the regulation of integrin β3 and β-1,4-galactosyltransferase protein expression was found to be most closely associated with atopic dermatitis pathogenesis. (4) The mechanism by which moxibustion treats atopic dermatitis was found to primarily involve signaling pathways related to infection or immunity, such as herpes simplex virus type 1 infection, olfactory transduction, influenza A, and steroid hormone biosynthesis. The most relevant proteins included nuclear factor κB subunit 1, major histocompatibility complex protein, RT1-Bb, Jak1, and Cdk6. These findings suggest that moxibustion exerts its intervention effect on atopic dermatitis through multi-targets, multi-pathways, and multi-mechanisms. It can reverse the inflammatory response induced by atopic dermatitis while exerting anti-inflammatory and antioxidant effects.


Key words: moxibustion, atopic dermatitis, rats, mechanisms of action, proteomics technology, bioinformatics

中图分类号: