中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5731-5734.doi: 10.3969/j.issn.1673-8225.2011.31.007

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

囊胚玻璃化冻存前激光打孔对冻融后移植效果的影响

方健叶，杨  菁，尹太郎，程  琰，赵庆红，邹宇洁，黄  伟   

  1. 武汉大学人民医院生殖医学中心，湖北省武汉市  430060
  • 收稿日期:2011-03-29 修回日期:2011-05-18 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 杨菁，博士，教授，博士生导师，武汉大学人民医院生殖医学中心，湖北省武汉市 430060 dryangqing@hotmail.com
  • 作者简介:方健叶★，女，1987年生，湖北省武汉市人，硕士，主要从事生殖医学研究。

Effect of laser drilling before vitrified cryopreservation on transplantation of frozen-thawed blastocyts

Fang Jian-ye, Yang Jing, Yin Tai-lang, Cheng Yan, Zhao Qing-hong, Zou Yu-jie, Huang Wei   

  1. Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
  • Received:2011-03-29 Revised:2011-05-18 Online:2011-07-30 Published:2011-07-30
  • Contact: Yang Jing, Doctor, Professor, Doctoral supervisor, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China dryangqing@hotmail.com
  • About author:Fang Jian-ye★, Studying for master’s degree, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China

PDF

419

被引次数

5

摘要：

背景：一系列研究均表明人工皱缩囊胚腔能明显提高囊胚的玻璃化冻融效果，但在皱缩过程中采用的物理方法和使用材料的不同可能会造成囊胚细胞的机械性损伤。
目的：探讨囊胚玻璃化冻存前激光打孔对冻融后移植效果的影响。
方法：3例不孕患者，平均年龄30岁，于囊胚玻璃化冻存前行激光打孔使囊胚皱缩，平均冻存时间为1年，确定移植前给予解冻。
结果与结论：3例患者中有2例取得了满意的妊娠结局，1例未妊娠。说明采用激光辅助打孔的方法使囊胚皱缩，结合玻璃化冻融后行囊胚移植安全有效。

关键词: 激光辅助孵化, 人工皱缩, 玻璃化冻融, 囊胚, 移植

Abstract:

BACKGROUND: A series of studies have demonstrated that artificially reduced blastocoelic cavity can greatly increase the vitrified frozen-thawed effects of blastocytes, but different physical methods and materials used during the process of reduction can lead to mechanical injury to blastocytes.
OBJECTIVE: To investigate the effects of laser drilling before vitrified cryopreservation of transplantation of frozen-thawed blastocyts.
METHODS: Blastocytes from three dysgenesia patients who averaged 30 years old were subjected to laser drilling before vitrified cryopreservation to reduce the blastocoelic cavity for 1 year on average. Before transplantation, these blastocytes were thawed.
RESULTS AND CONCLUSION: Two patients acquired satisfactory pregnancy and one patient did not get pregnancy. The results showed that laser drilling before vitrified cryopreservation reduces blastocoelic cavity and transplantation of frozen-thawed blastocytes is safe and effective.

中图分类号: