中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5722-5725.doi: 10.3969/j.issn.1673-8225.2011.31.005

• 肝移植 liver transplantation • 上一篇    下一篇

丹参对大鼠移植肝血红素氧合酶1表达的影响

高传长，李冬盛，王  恺，邹书兵   

  1. 南昌大学第二附属医院，江西省南昌市 330006
  • 收稿日期:2011-04-07 修回日期:2011-06-10 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 邹书兵，教授，主任医师，硕士生导师，南昌大学第二附属医院肝胆外科，江西省南昌市 330006 zousb999@163.com
  • 作者简介:高传长★，男，1985年生，湖南省麻阳县人，苗族，南昌大学医学院在读硕士，主要从事肝脏移植等方面的基础研究。 chuanzhang1985116@yahoo.cn

Effects of Danshen on heme oxygenase-1 expression in rat transplanted liver

Gao Chuan-chang, Li Dong-sheng, Wang Kai, Zou Shu-bing   

  1. Department of Hepatobiliary Surgery, Second Affiliated Hospital of Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • Received:2011-04-07 Revised:2011-06-10 Online:2011-07-30 Published:2011-07-30
  • Contact: Zou Shu-bing, Professor, Chief physician, Master’s supervisor, Department of Hepatobiliary Surgery, Second Affiliated Hospital of Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China zousb999@163.com
  • About author:Gao Chuan-chang★, Studing for master’s degree, Department of Hepatobiliary Surgery, Second Affiliated Hospital of Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China chuanzhang1985116@yahoo.cn

PDF

274

被引次数

7

摘要：

背景：器官移植前使用丹参预处理能够保护组织缺血-再灌注损伤，改善移植器官存活率。
目的：观察含丹参的冷灌注液对同种异体大鼠移植肝脏中血红素氧合酶1表达的影响，以及对供体肝脏缺血-再灌注损伤的保护作用。
方法：将SD雄性大鼠随机分成UW液组(术中使用UW液灌注保存)、丹参+UW液组(术中使用丹参+UW液灌注保存)、ZnPP预处理组(移植前24 h腹腔内注射ZnPP，术中使用丹参+UW液灌注保存)，建立稳定的大鼠同种异体肝移植模型。同时取10只正常大鼠作为正常对照。
结果与结论：丹参+UW液组和UW液组血清总胆红素、谷丙转氨酶、谷草转氨酶水平明显低于ZnPP预处理组(P < 0.01)。血红素氧合酶1mRNA及其蛋白在丹参+UW液组中较UW组表达更明显，在ZnPP预处理组中表达明显受到抑制(P< 0.05)。丹参+UW液组肝脏Suzuki标准评分明显低于ZnPP预处理组及UW液组(P < 0.05)。表明丹参能上调同种异体的大鼠移植肝脏中血红素氧合酶1 mRNA及其蛋白的表达，减轻供肝缺血-再灌注损伤，保护移植大鼠肝脏。

关键词: 血红素氧合酶1, 肝移植, 丹参, 缺血-再灌注损伤, 肝脏

Abstract:

BACKGROUND: Danshen pretreatment before organ transplantation can protect tissue from ischemia-reperfusion injury and improve the survival rate of grafts
OBJECTIVE: To investigate the effects of Danshen-containing cold perfusion solution on heme oxygenase-1 (HO-1) expression in rat liver allograft and the protective effects on donor liver from ischemia-reperfusion injury.
METHODS: Sprague-Dawley male rats were randomly divided into University of Wisconsin solution (UW) solution group (UW group, preservation using UW solution during transplantation), Danshen+UW solution group (DS+UW group, preservation with Danshen+UW solution during transplantation), ZnPP (HO-1 inhibitor) pretreatment group (ZnPP group, intraperitoneal injection of ZnPP 24 hours before transplantation, and preservation with Danshen+UW solution during transplantation). Reliable rat models of liver allografting were established. Ten normal rats were selected as normal controls.
RESULTS AND CONCLUSION: Serum total bilirubin, alanine aminotransferase, and aspartate aminotransferase levels were significantly lower in the DS+UW group, UW group than in the ZnPP group (P < 0.01). HO-1 mRNA and protein expressions were greater in the DS+UW group than in the UW group, but the expressions were obviously inhibited in the ZnPP group (P < 0.05). Suzuki scores were significantly lower in the DS+UW group than in the ZnPP group and UW group. These findings suggest that Danshen can upregulate HO-1 mRNA and protein expressions in rat liver allograft, alleviate ischemia-reperfusion injury of donor liver, and protect the transplanted liver.

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