中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (30): 5596-5599.doi: 10.3969/j.issn.1673-8225.2011.30.021

• 骨与关节图像与影像 bone and joint imaging • 上一篇    下一篇

脊髓损伤早期及制动大鼠股骨干骺端的显微CT观察

余文超，刘  岩，袁  文，郭永飞，曹振羽，邹翰林   

  1. 解放军第二军医大学长征医院骨科，上海市200003
  • 收稿日期:2011-03-30 修回日期:2011-05-03 出版日期:2011-07-23 发布日期:2011-07-23
  • 通讯作者: 刘岩，副教授，副主任医师，解放军第二军医大学长征医院骨科，上海市200003 liuyan318@yahoo.cn
  • 作者简介:余文超★，男，1985年生，江苏省镇江市人，解放军第二军医大学在读硕士，主要从事创伤外科研究。 yuwenchao0117@163.com
  • 基金资助:

    上海市科学技术委员会科研计划项目(08411965800)。

Micro CT observation on femoral metaphysis after spinal cord injury and immobilization in rats

Yu Wen-chao, Liu Yan, Yuan Wen, Guo Yong-fei, Cao Zhen-yu, Zou Han-lin   

  1. Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai  200003, China
  • Received:2011-03-30 Revised:2011-05-03 Online:2011-07-23 Published:2011-07-23
  • Contact: Liu Yan, Associate professor, Associate chief physician, Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China liuyan318@yahoo.cn
  • About author:Yu Wen-chao★, Studying for master’s degree, Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China yuwenchao0117@163.com
  • Supported by:

    Science Research Plan of Shanghai Science and Technology Committee, No. 08411965800*

PDF

362

被引次数

3

摘要：

背景：脊髓损伤后可引起损伤平面以下骨量大量丢失，导致骨质疏松。
目的：观察比较脊髓损伤及失用性制动模型大鼠股骨远端骨密度及骨微观结构的改变。
方法：将SD大鼠随机分为3组：对照组，切除T10椎板，不损伤硬膜及脊髓；脊髓损伤组，切除T10椎板后行Allen's法造成脊髓损伤；制动组，以大鼠双侧腿－尾缝合造成双下肢制动。10 d后取一侧尺、桡骨及股骨行骨密度检测，另一侧股骨行显微CT扫描。
结果与结论：脊髓损伤组与制动组大鼠股骨远端骨密度、骨矿物质含量、骨体积分数表、骨小梁厚度、骨皮质面积及厚度、骨小梁数量均低于对照组(P < 0.05 )，骨小梁结构模型指数、骨表面积体积比、骨小梁分离度均高于对照组；脊髓损伤组上述指标较制动组变化程度更显著(P < 0.05)。3组尺、桡骨密度差异无显著性意义。说明脊髓损伤及制动均可导致骨量丢失，在脊髓损伤早期损伤平面以下部位骨微观结构呈现骨质疏松明显改变，且程度比失用性因素严重。

关键词: 脊髓损伤, 显微CT, 股骨, 骨密度, 组织构建实验造模

Abstract:

BACKGROUND: Osteoporosis results from spine cord injury (SCI), due to a great deal of bone loss.
OBJECTIVE: To make an observation and comparison on bone density and bone microstructure at the distal femora changes after both SCI in early stage and osteoporosis due to immobilization.
METHODS: The SD rats were randomly divided into three groups. In the control group, T10 vertebrae plate resection was performed to rats without injuring dura mate of spinal cord and spinal cord. T10 vertebrae plate resection was also performed to rats in the SCI group with spinal cord injury by Allen’s method. Rats in the immobilization group were immobilized by limb-tail fixation at bilateral hindlimbs. The femoral metaphysis, ulna and radius were chosen for detection of bone density individuals and Micro CT scanning was performed to the other femoral metaphysis. 
RESULTS AND CONCLUSION: Compared with the control group, bone density, bone mineral content, bone volume/ total volume (BV/TV) , trabecular thickness (Tb.Th), cortical bone area (Ct.Ar), cortical thickness (Ct.Th), trabecular number (Tb.N) in the distal femur of rats in the SCI group and immobilization group deceased dramatically respectively (P < 0.05). Structure model index (SMI), bone surface/ total volume (BS/TV), trabecular separation/spacing (Tb.Sp) in the distal femur of the SCI group was higher than that of the control group (P < 0.05 ). No bone density difference of significance was found in the elbow-bone and radius between the three groups. Above all, both SCI and immobilization can result in osteoporosis. SCI have resulted in significantly changes in the bone microstructure in early stage and compared with the immobilization group, the degree of changes was more seriously.

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