中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (30): 5511-5515.doi: 10.3969/j.issn.1673-8225.2011.30.001

• 骨与关节损伤基础实验 basic experiments of bone and joint injury •    下一篇

经腰椎间盘的感受器通路

耿祝生1，2，章云海1，2，雷  玲1，崔吉正1，2，张小宝1，扬聪颖3，李  玉4，许新堂5   

  1. 连云港市第一人民医院，1麻醉科，2疼痛科，3病理科，4连云港市临床医学实验中心，5放射科，江苏省连云港市222002
  • 收稿日期:2011-05-24 修回日期:2011-06-30 出版日期:2011-07-23 发布日期:2011-07-23
  • 通讯作者: 章云海，主任医师，连云港市第一人民医院麻醉科，疼痛科，江苏省连云港市 222002 zyh1957423@sina.com
  • 作者简介:耿祝生，男，1969年生，江苏省连云港市人，汉族，1993年南京医学院毕业，副主任医师，主要从事急、慢性疼痛疾病诊治方面的研究。 Gengzs1898@sina.com
  • 基金资助:

    the National Natural Science Foundation of China, No. 30271260*; Third Opening Topics of Jiangsu Provincial Key Laboratory for Higher Education, No. K2093*; Instructive and Planning Topics of Science and Technology Department of Jiangsu Province, No. BS2002305*; Topics of Science and Technology Department of Lianyungang, No. SH0408*, SH0507*, SH0905*

A receptor pathway via the lumbar intervertebral disc

Geng Zhu-sheng1, 2, Zhang Yun-hai1, 2, Lei Ling1, Cui Ji-zheng1, 2, Zhang Xiao-bao1, Yang Cong-ying3, Li Yu4, Xu Xin-tang5   

  1. 1Department of Anesthesiology, 2Department of Pain, 3Department of Pathology, 5Department of Radiology, First People’s Hospital of Lianyungang City, Lianyungang  222002, Jiangsu Province, China; 4Lianyungang Clinical Laboratory Center, Lianyungang  222002, Jiangsu Province, China
  • Received:2011-05-24 Revised:2011-06-30 Online:2011-07-23 Published:2011-07-23
  • Contact: Zhang Yun-hai, Chief physician, Department of Anesthesiology, First People’s Hospital of Lianyungang City, Lianyungang 222002, Jiangsu Province, China zyh1957423@sina.com
  • About author:Geng Zhu-sheng, Associate chief physician, Department of Anesthesiology, First People’s Hospital of Lianyungang City, Lianyungang 222002, Jiangsu Province, China Gengzs1898@sina.com
  • Supported by:

    国家自然科学基金(30271260)：皮内注射局麻药远程镇痛机制的研究；江苏高校省级重点实验室第三批对外开放课题(K2093)：皮内给药镇痛机制的研究；江苏省科技厅指导计划课题(BS2002305)：皮内感受器通路与中医经络相关的神经基础研究；连云港市科技局课题(SH0408)：皮内注药治疗带状疱疹后遗神经痛机制研究；连云港市科技局课题(SH0507)：与胃牵涉区相关的皮部腧穴通路的研究；连云港市科技局课题(SH0905)：经腰椎关节突和椎板骨骼肌附着点感受器通路形态学研究。

PDF

600

被引次数

5

摘要：

背景：哪些经腰椎关节突和椎板骨骼肌附着点标记的神经元末梢可以进入椎间盘呢？椎间盘的感受器通路尚不清楚。
目的：观察家兔经腰椎间盘的感受器通路形态学特征。 
方法：8只家兔在CT引导下经L3~4腰椎椎间盘分别注射荧光逆行神经追踪剂核黄进行逆行神经追踪，按注射后18，36 h均分为2组，取不同部位连续冰冻切片观察神经末梢部位，并记录曝光时间。
结果与结论：家兔腰椎间盘感受器通路网络由交感神经节及节后神经元、小肠节细胞、脊神经节3大神经网络组成。作用的靶点分别在腰椎间盘纤维环、腰椎间盘髓核、腰椎间关节滑膜、胃黏膜、小肠黏膜、肌膜等，与腰椎关节突和椎板骨骼肌附着点的感受器通路在交感神经节及节后神经元、小肠节细胞、脊神经节、3大神经网络相重叠。且盘内注药可引起多个脊神经节内的炎症反应。

关键词: 腰椎间盘, 感受器通路, 逆行神经追踪, 家兔, 脊神经节

Abstract:

BACKGROUND: Which skeletal muscle labeled peripheral neurons at attachment points of the lumbar facet and lamina can enter the disc? The receptor pathway via the lumbar intervertebral disc is unclear.
OBJECTIVE: To observe the morphologic characteristics of the receptor pathway via the lumbar intervertebral disc.
METHODS: Injection of fluorescent retrograde nerve tracer into the L3-4 segments of eight rabbits was performed for retrograde nerve tracing. The rabbits were divided into two groups: one group at 18 hours after injection, and the other group at 36 hours after injection. Consecutive frozen sections from different zones were taken for observation of nerve end, and the exposure time was recorded.
REUSLTS AND CONCLUSION: Receptor pathway network of the lumbar intervertebral disc in rabbits was composed of sympathetic ganglia and postganglionic neurons, intestinal ganglion cells, and spinal ganglion. Targeted sites were lumbar disc annulus, lumbar intervertebral disc nucleus, lumbar synovial membrane, gastric mucosa, intestinal mucosa, fascia, etc., overlapped with the receptor pathway at the sympathetic ganglia and postganglionic neurons, intestinal ganglion cells, spinal ganglia. Disc administration can cause inflammatory reactions of many spinal ganglia.

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