中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (29): 5339-5342.doi: 10.3969/j.issn.1673-8225.2011.29.005

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

丝素蛋白/羟基磷灰石材料复合骨髓间充质干细胞构建组织工程化软骨

杨  耀1，徐卫袁2，张  亚3，张兴祥2，刘宗宝2，钱  辉2，黄建平2，崔志浩2   

  1. 1江苏大学临床医学院，江苏省镇江市 212013
    2张家港市第一人民医院骨科，江苏省张家港市  215600
    3苏州大学附属儿童医院，江苏省苏州市  215003
  • 收稿日期:2011-01-17 修回日期:2011-03-18 出版日期:2011-07-16 发布日期:2011-07-16
  • 通讯作者: 徐卫袁，博士，教授，主任医师，张家港市第一人民医院骨科，江苏省张家港市 215600 xwy143@163.com
  • 作者简介:杨耀★，男，1981年生，江苏省盐城市人，汉族，江苏大学在读硕士，主要从事脊柱外科和组织工程的研究。 yysun0102@sina.com

Silk fibroin/hydroxyapatite combined with bone marrow mesenchymal stem cells for construction of tissue engineered cartilage

Yang Yao1, Xu Wei-yuan2, Zhang Ya3, Zhang Xing-xiang2, Liu Zong-bao2, Qian Hui2, Huang Jian-ping2, Cui Zhi-hao2   

  1. 1School of Clinical Medicine, Jiangsu University, Zhenjiang  212013, Jiangsu Province, China
    2Department of Orthopaedics, First People’s Hospital of Zhangjiagang, Zhangjiagang  215600, Jiangsu Province, China
    3Children's Hospital Affiliated to Soochow University, Suzhou  215003, Jiangsu Province, China
  • Received:2011-01-17 Revised:2011-03-18 Online:2011-07-16 Published:2011-07-16
  • Contact: Xu Wei-yuan, Doctor, Professor, Chief physician, Department of Orthopaedics, First People’s Hospital of Zhangjiagang, Zhangjiagang 215600, Jiangsu Province, China xwy143@163.com
  • About author:Yang Yao★, Studying for master’s degree, School of Clinical Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China yysun0102@sina.com

PDF

311

被引次数

7

摘要：

背景：随着组织工程的兴起，软骨损伤的修复可能性显著地提高，但单一的支架材料均不能符合理想支架，有一定的局限性。
目的：观察骨髓间充质干细胞复合丝素蛋白/羟基磷灰石构建组织工程化软骨的可行性。
方法：体外分离培养骨髓间充质干细胞，并定向诱导成软骨细胞，与丝素蛋白/羟基磷灰石复合培养，构建膝关节胫骨平台全层关节软骨缺损。54只大白兔单侧膝关节全层软骨缺损模型后随机抽签法分为3组，复合组植入细胞-丝素蛋白/羟基磷灰石复合物；材料组植入单纯丝素蛋白/羟基磷灰石，对照组不行任何植入。植入后8，12周CT检查及组织学检查观察软骨缺损修复情况。
结果与结论：植入后8周，复合组关节面不平整，关节间隙增大，形成新生类软骨细胞，基质丰富。材料组关节面塌陷，软骨细胞少量增殖。植入后12周，复合组关节面平整，关节间隙如常。大量软骨细胞出现，与周边软骨色泽一样，支架材料完全降解。材料组关节面不平整，软骨细胞不完全充填，支架材料部分降解。对照组未见修复。提示用骨髓间充质干细胞复合丝素蛋白/羟基磷灰石可形成透明软骨修复动物膝关节全层软骨缺损，显示了丝素蛋白/羟基磷灰石材料作为关节软骨组织工程支架材料的良好生物相容性。

关键词: 丝素蛋白/羟基磷灰石材料, 骨髓间充质干细胞, 细胞诱导, 软骨缺损, 软骨组织工程

Abstract:

BACKGROUND: With the emergence of tissue engineering, the possibility of repairing the cartilage injury is greatly increased, but single scaffold materials fail to meet ideal scaffold, and have some limitations.
OBJECTIVE: To explore the feasibility of silk fibroin/hydroxyapatite combined with bone marrow mesenchymal stem cells (BMSCs) for construction of tissue engineered cartilage
METHODS: BMSCs were isolated, cultured and induced into chondrocytes, which were cocultured with silk fibrion/hydroxyapatite to construct full-thickness articular cartilage defects in the platform of knee joints tibial plateau. A total of 45 rabbits with unilateral knee full thickness cartilage defects model were randomly divided into 3 groups. The compound group was implanted with cell-silk fibrion/hydroxyapatite compound; material group was only implanted with silk fibrion/hydroxyapatite; implantation was not given in the control group. The reparation condition of cartilage defects was observed by CT examination and histological examination at 8, 12 weeks after implantation.
RESULTS AND CONCLUSION: In the compound group, the articular surface was not flat, the joint space was increased, formed new chondrocyte-like cells and extracellular matrix was extremely rich after 8 weeks. In the material group, articular surface was collapsed and a small amount of chondrocytes were proliferated. After 12 weeks, in the compound group, the articular surface was flat, the joint space was normal; a large number of chondrocytes were emerged, and the color of repaired tissues was closed to surrounding cartilage and scaffolds were completely degraded. In the material group, the articular surface was not flat and cartilage cells did not completely filled, some scaffolds were degraded. The defects were not repaired in the control group. It is indicated that the method of repairing the full-thickness hyaline cartilage defects using BMSCs combined with silk fibroin/hydroxyapatite is feasible, and the silk fibroin/hydroxyapatite can be used as scaffold materials in articular cartilage tissue engineering due to a good biocompatibility.

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