中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5303-5306.doi: 10.3969/j.issn.1673-8225.2011.28.043

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

肾上腺髓质素真核表达载体对皮瓣缺血再灌注损伤的影响

杨  锋1，刘志坤1，何  葵2   

  1. 1南华大学附属第二医院整形外科，湖南省衡阳市 421001
    2衡阳市中心医院，湖南省衡阳市  421001
  • 收稿日期:2011-03-22 修回日期:2011-06-02 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 何葵，博士，主任医师，衡阳市中心医院，湖南省衡阳市 421001 hawk_hk@126.com
  • 作者简介:杨锋★，男，1976年生，硕士，2010年南华大学毕业，湖南省浏阳县人，汉族，主治医师，目前主要从事整形美容的临床治疗。 doctoryangfeng@hotmail.com
  • 基金资助:

    课题受衡阳市科技局项目科研基金资助项目(2009KJ47)资助，项目名称：重组肾上腺髓质素对大鼠皮瓣缺血再灌注损伤的保护作用。

Effects of adrenomedullin eukaryotic expression vector on skin flap ischemia reperfusion injury

Yang Feng1, Liu Zhi-kun1, He Kui2   

  1. 1Department of Plastic Surgery, the Second Affiliated Hospital of University of South China, Hengyang   421001, Hunan Province, China
    2Hengyang Central Hospital, Hengyang   421001, Hunan Province, China
  • Received:2011-03-22 Revised:2011-06-02 Online:2011-07-09 Published:2011-07-09
  • Contact: He Kui, Doctor, Chief physician, Hengyang Central Hospital, Hengyang 421001, Hunan Province, China hawk_hk@126.com
  • About author:Yang Feng★, Master, Attending physician, Department of Plastic Surgery, the Second Affiliated Hospital of University of South China, Hengyang 421001, Hunan Province, China doctoryangfeng@ hotmail.com
  • Supported by:

    a grant from Scientific Research Foundation of Science and Technology Bureau of Hengyang City, No. 2009KJ47*

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被引次数

1

摘要：

背景：肾上腺髓质素可在心、脑、肾、肝等器官的缺血再灌注过程中起保护作用。
目的：观察肾上腺髓质素真核表达载体对大鼠腹部皮瓣缺血再灌注损伤的影响及其作用机制。
方法：构建大鼠肾上腺髓质素真核表达载体，将SD大鼠随机分假手术组、模型组、维拉帕米组、重组质粒组。重组质粒组腹部皮内注射肾上腺髓质素真核表达载体，其余各组注射等量生理盐水，各组注射4次后建立缺血再灌注模型，维拉帕米组于皮瓣内注射维拉帕米。
结果与结论：与模型组相比，重组质粒组皮瓣组织中丙二醛、血管内皮素1的含量分别降低了38.50%(P  < 0.01)，54.73%  (P  < 0.01)，超氧化物歧化酶的含量增加了50.67%(P < 0.05)。皮瓣组织学检查结果显示重组质粒组皮瓣炎性细胞浸润与水肿程度也较模型组明显减轻。结果证实肾上腺髓质素真核表达载体对大鼠皮瓣缺血再灌注损伤具有保护作用，其机制与减轻脂质过氧化及减少炎症细胞浸润有关。

关键词: 肾上腺髓质素, 缺血再灌注, 真核表达, 皮瓣, 组织构建

Abstract:

BACKGROUND: Adrenomedullin plays protective effects on ischemia and reperfusion injury of the heart, brain, kidney and liver.
OBJECTIVE: To explore the effects of adrenomedullin eukaryotic expression vector pVAX1-ADM on ischemia reperfusion injury in rat abdominal flaps and the possible mechanism of action.
METHODS: Rat adrenomedullin eukaryotic expression vector pVAX1-ADM was reconstructed. Rats were randomly divided into four groups: sham-operated, model, Verapamil, and pVAX1-ADM. Rats in the pVAX1-ADM group were injected with recombinant plasmid (600 μg) and rats in the other three groups were injected with the same amount of normal saline, once a week. Four weeks later, ischemia reperfusion models were established by 9 hours of ischemia followed by 12 hours of reperfusion. Before reperfusion, the Verapamil group was injected with Verapamil. At 7 days after surgery, the contents of malondialdehyde (MDA), superoxide dismutase (SOD) and endothelium 1 (ET-1) in targeted flaps were measured, and histomorphological examination of the targeted flaps was performed.
RESULTS AND CONCLUSION: Compared with the model group, the content of MDA and ET-1 in the pVAX1-ADM group abdominal flaps was decreased by 38.50% (P < 0.01) and 54.73% (P < 0.01) respectively, and activity of SOD was increased by 50.67% (P < 0.05). Compared with the model group, only a few inflammatory cells infiltrated and interstitial edema decreased significantly in the flaps in the PVAX1-ADM group. These findings suggest that adrenomedullin eukaryotic expression vector PVAX1-ADM exhibits protective effects on skin flap ischemia reperfusion injury, and the underlying mechanism is related to reduction in lipid peroxidation and inflammatory cell infiltration.

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