中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5245-5248.doi: 10.3969/j.issn.1673-8225.2011.28.029

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

脉冲电场对人宫颈癌Hela细胞侵袭转移能力的影响

张  琴1，熊正爱1，周  玮1，李成祥2，姚陈果2   

  1. 1重庆医科大学附属第二医院妇产科，重庆市  400010
    2重庆大学，重庆市 400030
  • 收稿日期:2011-01-16 修回日期:2011-03-24 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 熊正爱，博士生导师，重庆医科大学附属第二医院妇产科，重庆市 400010 xiongzhengai61@21cn.com
  • 作者简介:张琴★，女，1977年生，四川省成都市人，汉族，重庆医科大学在读硕士，主要从事妇科肿瘤方向的研究。 20406737@qq. com
  • 基金资助:

    国家自然科学基金：陡脉冲不可逆性电击穿治疗肿瘤的作用机理及关键技术研究(50637020)。

Pulse electric fields effect on invasion and metastasis of Hela cells in human cervical cancer

Zhang Qin1, Xiong Zheng-ai1, Zhou Wei1, Li Cheng-xiang2, Yao Chen-guo2   

  1. 1Deparment of Gynaecology and Obsterics, Second Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
    2Chongqing University, Chongqing  400030, China
  • Received:2011-01-16 Revised:2011-03-24 Online:2011-07-09 Published:2011-07-09
  • Contact: Xiong Zheng-ai, Doctoral supervisor, Department of Gynaecology and Obsterics, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China xiongzhengai61@21cn.com
  • About author:Zhang Qin★, Studying for master’s degree, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China 20406737@qq.com
  • Supported by:

    the National Natural Science Foundation of China, No. 50637020*

PDF

340

被引次数

2

摘要：

背景：脉冲电场对肿瘤细胞侵袭转移能力是否具有抑制作用尚不十分清楚。
目的：探讨不同强度脉冲电场对人宫颈癌Hela细胞侵袭转移能力的影响及可能的机制。
方法：以场强分别为0，500，1 000和1 500 V/cm的脉冲电场作用人宫颈癌Hela细胞后，采用平板克隆形成实验，观察脉冲电场对细胞克隆能力的影响；采用Transwell小室进行人工重组基底膜侵袭和转移实验，观察脉冲电场对细胞侵袭和转移能力的影响；采用RT-PCR法检测宫颈癌Hela细胞中基质金属蛋白酶2和RhoC基因mRNA的表达情况。
结果与结论：脉冲电场作用后，宫颈癌Hela细胞的克隆能力受到抑制；细胞侵袭和转移能力明显降低；基质金属蛋白酶2和RhoC基因mRNA的表达均明显减弱，且有电场强度依赖性。结果表明，脉冲电场能抑制人宫颈癌Hela细胞的侵袭转移能力，其机制可能与降低RhoC和基质金属蛋白酶2的表达有关。

关键词: 脉冲电场, 电穿孔, 宫颈癌, 侵袭, 转移, 基质金属蛋白酶2, RhoC

Abstract:

BACKGROUND: The inhibitory effect of pulse electric fields on invesion and metastasis ability of tumor cells is unclear.
OBJECTIVE: To explore the impact of different intensity pulse electric fields on the human cervical carcinoma Hela cell invasion and metastasis and its possible mechanism.
METHODS: The electric fields with intensity of 0, 500, 1 000 and 1 500 V/cm were performed on the human cervical carcinoma Hela cell. The clone capacity was measured with flat clone formation experiment; the invasion and immigration capacity was evaluated with transwell cave method;the expression level of mRNA of MMP-2 and RhoC was detected by RT-PCR method．
RESULTS AND CONCLUSION: After being treated with pulsed electric fields, the clone capacity of Hela cells was inhibited. The invasion and immigration capacity of Hela cells were also inhibited. The expression level of mRNA of matrix metalloproteinase 2 (MMP-2) and RhoC were decreased. The pulse electric fields could affect the invasion and metastasis capacity of the human cervical cancer Hela cells. It is probably one of its molecular mechanisms to inhibit the expression of MMP-2 and RhoC.

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