中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5233-5236.doi: 10.3969/j.issn.1673-8225.2011.28.026

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

基因表达谱差异分析丹黄散对糖尿病足创面修复的作用

王安宇，徐寒松，赵  胜，杨传经，毛  奇，乔艺杰，魏良纲   

  1. 贵阳中医学院第二附属医院代谢内分泌科，贵州省贵阳市  550002
  • 收稿日期:2011-04-06 修回日期:2011-05-16 出版日期:2011-07-09 发布日期:2011-07-09
  • 作者简介:王安宇☆，男，1976年生，贵州省贵阳市人，汉族，2005年中南大学毕业，博士，副教授，主要从事糖尿病足的中西医结合临床与基础研究。 anyuw@tom. com
  • 基金资助:

    贵州省科技厅基金(黔科合J字[2006]2067号)，贵州省中医药管理局资助课题。课题名称：“糖尿病足溃疡组织基因谱表达以及丹黄散干预的影响”。

Analysis on effects of Danhuangsan on wound surface repair of diabetic feet by gene expression profiling

Wang An-yu, Xu Han-song, Zhao Sheng, Yang Chuan-jing, Mao Qi, Qiao Yi-jie, Wei Liang-Gang   

  1. Department of Metabolism and Endocrinology, Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang   550002, Guizhou Province, China
  • Received:2011-04-06 Revised:2011-05-16 Online:2011-07-09 Published:2011-07-09
  • About author:Wang An-yu, Doctor, Associate professor, Department of Metabolism and Endocrinology, Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China anyuw@tom.com
  • Supported by:

    a grant from Science and Technology Department of Guizhou Province, No.J[2006]2067*; a grant from Guizhou Administration Bureau of Traditional Chinese Medicine*

PDF

302

被引次数

18

摘要：

背景：丹黄散能刺激肉芽组织增生，减少溃疡创面分泌物，促进溃疡创面修复。
目的：从基因组层面评估丹黄散在糖尿病足治疗中的作用。
方法：纳入2007-10/2008-09于贵阳中医学院第二附属医院代谢与内分泌科就诊的糖尿病足患者9例，分别给予常规治疗(糖尿病足组)及丹黄散外敷治疗(丹黄散治疗组)；同时选取同时期的外伤患者作为对照组。取以上3组患者足组织标本，应用Oligo GEArray®芯片检测各组基因表达谱，比较分析基因表达谱差异。
结果与结论：和对照组比较，糖尿病足组有28个基因表达上调，6个基因表达下调；与糖尿病足组比较，丹黄散治疗组有30个基因表达上调，4个基因表达下调。说明糖尿病足溃疡局部涉及多基因差异表达，丹黄散可调控部分差异基因表达。

关键词: 丹黄散, 糖尿病足, 基因谱, 基因芯片, 创面, 修复

Abstract:

BACKGROUND: Danhuangsan can stimulate the hyperplasia of granulation tissue, reduce the secretion of ulcerative wound surface, and promote the repair of ulcer wound surface.
OBJECTIVE: To investigate the clinical effects of Danhuangsan on treatment of diabetic feet from the level of genome.
METHODS: Nine patients with diabetic feet who received treatment at the Department of Metabolism and Endocrinonolgy, Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine from October 2007 to September 2008 were included in this study and were randomly divided into a conventional therapy group and a Danhuangsan therapy group. Traumatic patients who received treatment concurrently were selected as the controls. Foot tissue samples were taken from three groups. Gene expression profile was detected with Oligo GEArray® gene chip and the gene expression profile difference was compared.
RESULTS AND CONCLUSION: Compared with the control group, there were 28 genes with upregulated expression and 6 genes with downregulated expression in the conventional therapy group. Compared with the conventional therapy group, there were 30 genes with upregulated expression and 4 genes with downregulated expression in the Danhuangsan therapy group. These findings suggest that there are many differentially expressed genes at the ulcerative tissue region of the diabetic feet, and Danhuangsan can regulate the differential expression of some genes.

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