中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4511-4515.doi: 10.3969/j.issn.1673-8225.2011.24.035

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    下一篇

骨形态发生蛋白7基因变异与新疆维吾尔族肥胖人群的相关性

严治涛1，李南方2，郭艳英2，姚晓光2，王红梅2，胡君丽2   

  1. 1新疆医科大学研究生院，新疆维吾尔自治区乌鲁木齐市 830000
    2新疆维吾尔自治区人民医院高血压科，新疆高血压诊断治疗研究中心，新疆维吾尔自治区乌鲁木齐市 830001
  • 收稿日期:2011-03-25 修回日期:2011-05-03 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 李南方，博士，主任医师，教授，博士生导师，新疆维吾尔自治区人民医院高血压科，新疆高血压诊断治疗研究中心，新疆维吾尔自治区乌鲁木齐市 830001 lnanfang@yahoo.com.cn
  • 作者简介:严治涛☆，男，汉族，1977年出生，新疆维吾尔自治区石河子市人，新疆医科大学在读博士，主治医师，主要从事高血压分子流行病学研究。 greenbloodsword@yahoo.com.cn
  • 基金资助:

    新疆维吾尔自治区重点实验室“新疆高血压病研究实验室”开放课题(XJYS0906-2009-03)，新疆自治区人民医院青年基金项目(20090204)。

Genetic variations of bone morphogenetic protein 7 associated with obesity in Xinjiang Uygur populations

Yan Zhi-tao1, Li Nan-fang2, Guo Yan-ying2, Yao Xiao-guang2, Wang Hong-mei2, Hu Jun-li2   

  1. 1Xinjiang Medical University Xinjiang Postgraduate College, Urumqi  830000, Xinjiang Uygur Autonomous Region, China
    2Department of Hypertension of the People's Hospital of Xinjiang Uygur Autonomous Region, Research Center of Diagnosis and Treatment of Hypertension, Urumqi  830000, Xinjiang Uygur Autonomous Region, China
  • Received:2011-03-25 Revised:2011-05-03 Online:2011-06-11 Published:2011-06-11
  • Contact: Li Nan-fang, Doctor, Chief physician, Professor, Doctoral supervisor, Department of Hypertension of the People's Hospital of Xinjiang Uygur Autonomous Region, Research Center of Diagnosis and Treatment of Hypertension, Urumqi 830000, Xinjiang Uygur Autonomous Region, China lnanfang2009@hotmail.com
  • About author:Yan Zhi-tao☆, Studying for doctorate, Attending physician, Xianjiang Medical University Postgraduate College, Urumqi 830000, Xinjiang Uygur Autonomous Region, China greenbloodsword@yahoo.com.cn
  • Supported by:

    the Open Topic of the Key Laboratory of Hypertension in Xinjiang Uygur Autonomous Region, No. XJYS0906- 2009-03*; the Youth Foundation Program of People’s Hospital of Xinjiang Uygur Autonomous Region, No. 20090204*

PDF

281

被引次数

4

摘要：

背景：研究表明骨形态发生蛋白7基因定位的染色体20q13是肥胖的易感区域，功能与基因定位均表明骨形态发生蛋白7基因是肥胖的易感基因。
目的：探讨骨形态发生蛋白7基因变异与新疆维吾尔族肥胖人群的关系。 
方法：以流行病学调查为基础的病例-对照研究，选取1 063例维吾尔族人群作为研究对象。先在48例维吾尔族肥胖人群(男/女：24/24)中测序筛查骨形态发生蛋白7基因功能区的变异位点，选取代表性变异位点应用TaqMan-PCR技术在研究人群中进行基因型鉴定并开展病例-对照关联研究。 
结果与结论：在骨形态发生蛋白7基因的功能区共发现5个新的和8个已知的变异位点。骨形态发生蛋白7基因的2个代表性变异位点rs60254222和rs17480735均符合Hardy-Weinberg平衡。老年人群中rs60254222变异的AA、AG、GG基因型在肥胖组及正常对照组中的频率分布分别为32.2%，55.7%，12.2%和23.8%，48.3%，27.9%，且两组间频率分布差异有显著性意义(P < 0.01)。老年人群中rs60254222变异不同基因型组间体质量指数、腰围、腰臀比差异有显著性意义(P < 0.05)。Logistic分析提示老年人群中rs60254222变异GG基因型是肥胖的保护性因素(OR=0.578，95%可信区间为0.401~0.833，P=0.003)。结果表明骨形态发生蛋白7基因变异位点rs60254222可能与新疆维吾尔族人群肥胖相关。

关键词: 骨形态发生蛋白7, 基因多态性, 肥胖, 维吾尔族, 病例-对照研究

Abstract:

BACKGROUND: Studies show that bone morphogenetic protein 7 (BMP-7) gene maps to chromosome 20q13, a susceptibility locus for obesity. BMP-7 represents a strong biological and positional candidate for a susceptibility factor for obesity.
OBJECTIVE: To investigate the association between the genetic variations of functional region in BMP-7 gene and obesity in Xinjiang Uygur populations.
METHODS: A case-control study was conducted based on epidemiological investigation. 1063 Uygur populations were selected. The exons, segmental introns and the promoter regions of BMP-7 gene were sequenced in 48 Uygur obese patients (male/female: 24/24). Representative variations were selected according to the minor allele frequency and linkage disequilibrium and genotyped using the TaqMan polymerase chain reaction method in 1063 Uygur individuals, a relatively isolated general population in a relatively homogeneous environment and a case-control study was conducted to analyze the association between genetic variations of BMP-7 gene and obesity.
RSSULTS AND CONCLUSION: Five novel and eight known variations in the BMP-7 gene were identified. Two genotype distributions, rs60254222 and rs17480735, followed Hardy-Weinberg equilibrium. There were significant differences in AA, AG, GG genotype distribution frequency of rs6025422 variation between obese and control groups in old adults (32.2%, 55.7%, 12.2% vs. 23.8%, 48.3%, 27.9%; P < 0.01)．There were significant differences in means of body mass index, waist circumference and waist-to-hip ratio were significantly decreased among individuals with AA, AG and GG genotypes of rs60254222 in old adults (P < 0.05). Logistic regression analysis showed that GG genotype of rs60254222 variation might be a obesity protective factor in old adults (OR = 0.578, 95% confidence interval 0.401-0.833, P = 0.003). Results showed that rs60254222 polymorphism in BMP-7 gene may be associated with obesity in Uygur populations.

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