中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4485-4488.doi: 10.3969/j.issn.1673-8225.2011.24.028

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

耐力运动大鼠跟腱胶原、基质金属蛋白酶1及其抑制剂1的表达

李  敏，金  力   

  1. 商丘师范学院体育学院，河南省商丘市  476000
  • 收稿日期:2011-02-19 修回日期:2011-04-26 出版日期:2011-06-11 发布日期:2011-06-11
  • 作者简介:李敏☆，女，1974年生，河南省商丘市人，汉族，2008年苏州大学毕业，博士，副教授，主要从事运动人体科学方面的研究。 liminxz74@163.com

Effect of treadmill exercise on the collagen, matrix metalloproteinases-1 and tissue inhibitors of metalloproteinase-1 mRNA in rat’s Achilles tendon

Li Min, Jin Li   

  1. School of Sports Science, Shangqiu Normal College, Shangqiu  476000, Henan Province, China
  • Received:2011-02-19 Revised:2011-04-26 Online:2011-06-11 Published:2011-06-11
  • About author:Li Min☆, Doctor, Associate professor, School of Sports Science, Shangqiu Normal College, Shangqiu 476000, Henan Province, China liminxz74@163.com

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360

被引次数

7

摘要：

背景：胶原是肌腱细胞外基质中的主要成分，基质金属蛋白酶是降解肌腱细胞外基质蛋白的重要蛋白酶。
目的：观察12周跑台运动对大鼠跟腱胶原、胶原Ⅰ、基质金属蛋白酶1及其抑制剂1表达的影响。
方法：将20只雄性SD大鼠随机分为对照组和运动组。第1周每天运动20 min，速度由12 m/min递增至20 m/min；以后跑台速度均为20 m/min，第2周坡度为5%，时间为30 min，第3~12周坡度为10%，时间为40 min，共运动12周。对照组正常饲养。
结果与结论：末次运动后24 h取大鼠跟腱。与对照组比较，运动组大鼠跟腱前胶原Ⅰα1、基质金属蛋白酶1及其抑制剂1 mRNA的表达明显升高(P < 0.05或P < 0.01)，羟脯氨酸的含量也有所增多，但与对照组比较差异无显著性意义(P > 0.05)。表明长期耐力运动可以提高跟腱中胶原的合成和降解能力。

关键词: 跑台运动, 跟腱, 胶原, 基质金属蛋白酶1, 基质金属蛋白酶组织抑制剂1

Abstract:

BACKGROUND: Collagen is the main protein component of tendon tissue. Metalloproteinases are a large family of enzymes capable of degrading all of the tendon matrix components.
OBJECTIVE: To investigate the effects of 12-week treadmill running on content of collagen and the expression of procollagen I, matrix metalloproteinases-1 (MMP-1) and tissue inhibitors of metalloproteinase-1 of rat Achilles tendon.
METHODS: A total of 20 male Sprague-Dawley rats were randomly assigned to control and exercise groups. At 1 week, exercise was performed for 20 minutes daily, at the increased speed from 12 m/min to 20 m/min. The treadmill speed was kept at 20 m/min. At 2 weeks, the gradient was 5%, and time was 30 minutes. At 3-12 weeks, the gradient was 10%, and the time was 40 minutes, for totall 12 weeks. In control group, the animals were fed normally.
RESULTS AND CONCLUSION: 24 hours after the last exercise, the rat Achilles tendon was obtained. Compared with control group, the expression of procollagen Iα1, MMP-1 and its inhibitor 1 mRNA was significantly increased (P < 0.05 or P < 0.01) in the exercise group, and the hydroxyproline content was increased (P > 0.05). These results show that long-term treadmill running can increase the synthesis and degradation of collagen in achilles tendon.

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