中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4423-4426.doi: 10.3969/j.issn.1673-8225.2011.24.013

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

磷酸化p38丝裂原活化蛋白激酶与转化生长因子β1在正常和腰椎间盘退变组织中的表达

原  野1，赵  静2，赵  杰2，李永民1，王  旭1   

  1. 1唐山市第二医院脊柱外科，河北省唐山市 063000
    2河北联合大学病理教研室，河北省唐山市 063000 
  • 收稿日期:2011-03-07 修回日期:2011-05-06 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 赵静，硕士，副教授，硕士研究生导师，河北联合大学病理教研室，河北省唐山市 063000 yuanyets@163.com
  • 作者简介:原野★，男，1971年生，副主任医师，1996年华北煤炭医学院毕业，硕士，主要从事脊柱外科临床工作。 yuanyets@163.com 并列第一作者：赵静，硕士，副教授，硕士研究生导师，河北联合大学病理教研室，河北省唐山市 063000 yuanyets@163.com
  • 基金资助:

    课题受河北省教育厅自然科学指导计划项目(Z2005102)和河北省科学技术研究与发展指导项目(052761422)资助，课题名称分别为“TGF-1，MMPs-1，MMPs-3在退变椎间盘组织中的表达及相关性研究”和“bFGF/TGF，MMPs/TIMPs在退变椎间盘组织中的表达研究”。

Phosphorylated p38 mitogen-activated protein kinase and transforming growth factor beta-1 expression in degenerative versus normal disc tissues

Yuan Ye1, Zhao Jing2, Zhao Jie2, Li Yong-min1, Wang Xu1   

  1. 1Department of Spine Surgery, Tangshan Second Hospital, Tangshan  063000, Hebei Province, China
    2Department of Pathology, Hebei Union University, Tangshan  063000, Hebei Province, China
  • Received:2011-03-07 Revised:2011-05-06 Online:2011-06-11 Published:2011-06-11
  • Contact: Zhao Jing, Master, Associate professor, Master’s supervisor, Department of Pathology, Hebei Union University, Tangshan 063000, Hebei Province, China yuanyets@163.com
  • About author:Yuan Ye★, Master, Associate chief physician, Department of Spine Surgery, Tangshan Second Hospital, Tangshan 063000, Hebei Province, China yuanyets@163.com Zhao Jing, Master, Associate professor, Master’s supervisor, Department of Pathology, Hebei Union University, Tangshan 063000, Hebei Province, China yuanyets@163.com Yuan Ye and Zhao Jing contributed equally to this paper.
  • Supported by:

     the Natural Science Foundation of Education Department of Hebei Province, No. Z2005102*; the Science and Technology Development Program of Hebei Province, No. 052761422*

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摘要：

背景：研究表明p38丝裂原活化蛋白激酶信号转导通路与椎间盘退变过程中炎症反应密切相关。
目的：检测腰退变椎间盘组织和正常腰椎间盘组织中磷酸化p38丝裂原活化蛋白激酶和转化生长因子β1的表达。
方法：选取经手术切除的36例退变椎间盘组织和10例正常椎间盘组织为对象，应用免疫组织化学法检测两组中磷酸化p38丝裂原活化蛋白激酶和转化生长因子β1的表达，并应用真彩色病理图像分析系统进行分析。
结果与结论：腰退变椎间盘组织中磷酸化p38丝裂原活化蛋白激酶和转化生长因子β1的阳性表达率均高于正常椎间盘组织 (P < 0.05)，证实磷酸化p38丝裂原活化蛋白激酶和转化生长因子β1在退变椎间盘组织中呈高表达。

关键词: 磷酸化p38丝裂原活化蛋白激酶, 腰退变椎间盘组织, 转化生长因子&beta, 1, 免疫组织化学, 骨组织工程

Abstract:

BACKGROUND: Studies show that p38 mitogen activated protein kinase (MAPK) signal pathway is highly correlated with inflammatory reactions during intervertebral disc degeneration.
OBJECTIVE: To study the phosphorylated p38MAPK (p-p38MAPK) and transforming growth factor β1 (TGF-β1) expression in patients with intervertebral disc degeneration.
METHODS: Degenerative disc tissues resected by surgery from 36 patients with lumbar disc herniation were selected and normal disc tissues from 10 cases of trauma were used as control group. The expression of p-p38MAPK and TGF-β1 was evaluated with immunohistochemistry method and analyzed using pathological imaging analysis system.
RESULTS AND CONCLUSION: The positive rate of p-p38MAPK and TGF-β1 expression in degenerative disc tissues was greater than normal disc tissue (P < 0.05). Results demonstrated that p-p38MAPK and TGF-β1 are highly expressed in the process of disc degeneration.

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