中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4367-4370.doi: 10.3969/j.issn.1673-8225.2011.23.043

• 干细胞临床实践 clinical practice of stem cells • 上一篇    

自体骨髓单个核细胞肝内移植治疗失代偿期肝硬化

郭学军，林晓燕，何玉卓，冯雪琴，李风菊，姚志琴   

  1. 濮阳市油田总医院血液科，河南省濮阳市  457001
  • 收稿日期:2011-02-26 修回日期:2011-04-21 出版日期:2011-06-04 发布日期:2011-06-04
  • 作者简介:郭学军，男，1967年生，河南省密县人，汉族，1990年浙江医科大学毕业，主任医师，教授，硕士生导师，主要从事骨髓间充质干细胞的诱导分化及临床应用。 pygxj@163.com
  • 基金资助:

    2010年濮阳市杰出人才创新基金项目(100308)。

Intrahepatic transplantation of autologous bone marrow mononuclear cells in treatment of liver cirrhosis in decompensatory period 

Guo Xue-jun, Lin Xiao-yan, He Yu-zhuo, Feng Xue-qin, Li Feng-ju, Yao Zhi-qin   

  1. Department of Hematology, Puyang General Hospital of Oil Field, Puyang 457001, Henan Province, China
  • Received:2011-02-26 Revised:2011-04-21 Online:2011-06-04 Published:2011-06-04
  • About author:Guo Xue-jun, Chief physician, Professor, Master’s supervisor, Department of Hematology, Puyang General Hospital of Oil Field, Puyang 457001, Henan Province, China pygxj@163.com
  • Supported by:

    the Innovation Foundation of Excellent Intellectual of Puyang City in 2010, No. 100308*

PDF

380

被引次数

10

摘要：

背景：大量研究发现，骨髓间充质干细胞是肝干细胞的主要肝外来源，骨髓间充质干细胞在特定环境下可分化为肝组织和肝细胞从而参加肝脏的修复与重建。
目的：观察自体骨髓单个核细胞移植治疗失代偿期肝硬化的效果及安全性。
方法：选择肝硬化失代偿期患者20例，在无菌条件下由髂后上棘抽取骨髓200 mL，在体外分离纯化骨髓单个核细胞并制成10 mL细胞悬液，经肝动脉将细胞悬液移植入肝脏，分别在移植后第2、4、8、12周复查肝脏功能，观察实验室指标，临床症状及不良反应。
结果与结论：移植后2周内所有血清学指标均无显著改变，移植后4周白蛋白由(27.05±5.23) g/L升到(30.02±5.02) g/L，纤维蛋白原由(1.55±0.53) g/L升到(2.55±0.53) g/L，凝血酶原时间由(24.05±5.23) s降到 (17.05±5.13) s，胆碱酯酶和甲胎蛋白也有不同程度上升，氨基转移酶、总胆红素水平在移植前后无显著性变化。单个核细胞移植后食欲改善、体力好转10例，腹胀减轻6例，腹水减少7例，下肢浮肿减轻4例。全部患者在移植中未发生严重并发症，移植近期无不良反应出现。

关键词: 自体骨髓单个核细胞, 移植, 肝硬化, 失代偿期, 血清学指标

Abstract:

BACKGROUND: Numerous studies have shown that bone marrow mesenchymal stem cells (BMSCs) are the main source of hepatic stem cells except for liver. BMSCs can differentiate into hepatic tissues and hepatic cells under a certain condition, and participate in the repair and reconstitution of the liver.
OBJECTIVE: To observe the effect and safety of autologous bone marrow mononuclear cell transplantation in treatment of cirrhosis in decompensatory period.
METHODS: A total of 20 patients with cirrhosis in decompensatory period were selected. 200 mL bone marrow was aspirated from posterior superior iliac spine in aseptic conditions. Bone marrow mononuclear cells were isolated and purified in vitro and made into 10 mL cell suspension. The stem cells were transplanted into the liver via hepatic artery. Liver function was rechecked at 2, 4, 8 and 12 weeks after transplantation. Laboratory data, clinical symptoms and adverse effects were observed.
RESULTS AND CONCLUSION: No significant changes in serological indexes were detected within 2 weeks after transplantation. The levels of albumin were increased from (27.05±5.23) g/L to (30.02±5.02) g/L; fibrinogen was increased from (1.55±0.53) g/L to (2.55±0.53) g/L; prothrombin time was deceased from (24.05±5.23) s to (17.05±5.13) s; cholinesterase and alpha fetoprotein was increased at different levels; no significant changes in aminoleucine transferase and total bilirubin levels were detectable. Appetite was improved and physical capacity was recovered in 10 cases; abdominal distention was alleviated in 6 cases; ascites was decreased in 7 cases; lower extremity edema was decreased in 4 cases after transplantation. There were no serious intraoperative complications. Patinets had no adverse reaction.

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