中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4351-4354.doi: 10.3969/j.issn.1673-8225.2011.23.039

• 干细胞临床实践 clinical practice of stem cells • 上一篇    下一篇

两种血细胞分离机干/祖细胞采集和移植效果的比较

叶书来1，应美爱2，陈家萍2，耿良权3   

  1. 安徽省立医院，1南区检验科，2输血科，3血液科，安徽省合肥市230001
  • 收稿日期:2010-12-14 修回日期:2011-01-31 出版日期:2011-06-04 发布日期:2011-06-04
  • 作者简介:叶书来，男，1962年生，安徽省桐城市人，汉族，1987年安徽医科大学毕业，副主任技师，主要从事临床输血和外周血干细胞采集研究。 yeshulai@yahoo.cn

Comparison of two blood cell separators in collection of peripheral blood stem cell/progenitor cells and effectiveness of transplantation

Ye Shu-lai1, Ying Mei-ai2, Chen Jia-ping2, Geng Liang-quan3   

  1. 1Clinical Laboratory, 2Department of Blood Transfusion, 3Department of Haematology, Anhui Provincial Hospital, Hefei  230001, Anhui Province, China
  • Received:2010-12-14 Revised:2011-01-31 Online:2011-06-04 Published:2011-06-04
  • About author:Ye Shu-lai, Associate chief technician, Clinical Laboratory, Anhui Provincial Hospital, Hefei 230001, Anhui Province, China yeshulai@yahoo.cn

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摘要：

背景：外周血造血干/祖细胞通过血细胞分离机进行体外收集，血细胞分离机的性能、工作状况将直接关系到采集物的特性和数量，从而直接影响受者的造血重建。
目的：比较Fenwal CS-3000 Plus与Amicus两种血细胞分离机采集外周血造血干/祖细胞及受者移植效果。
方法：共入选接受异基因外周血干细胞移植患者51例，Fenwal CS-3000 Plus组27例，平均年龄(34.2±10.6)岁；Amicus组24例，平均年龄(35.4±12.1)岁。比较两组采集物有核细胞数、CD34+细胞数、采集效率、红细胞与血小板采集量及造血重建时间。
结果与结论：两组采集物的有核细胞数、CD34+细胞数、采集效率、红细胞采集量及造血重建时间差异均无显著性意义；Fenwal CS-3000Plus组血小板采集量明显高于Amicus组(P < 0.01)。结果显示Fenwal CS-3000 Plus与Amicus血细胞分离机在分离外周血干/祖细胞方面和移植效果没有差异；血小板较低被采集者使用Amicus血细胞分离机采集可能更为安全。

关键词: 血细胞分离机, 外周血造血干/祖细胞, CD34+细胞, 分离效果, 移植

Abstract:

BACKGROUND: Peripheral blood stem cells/progenitor cells were collected in vitro using blood cell separators, which directly affects the features and quantity of collected cells, additionally, affects hematopoietic reconstitution of recipients. 
OBJECTIVE: To evaluate the efficiencies of Fenwal CS-3000 Plus and Amicus blood cell separators in collecting peripheral blood stem cells/progenitor cells and effectiveness of transplantation.
METHODS: Totally 51 cases received allogenic peripheral blood stem cells transplantation were selected, including 27 cases, mean aged (34.2±10.6) years, who performed stem cell apheresis procedures using Fenwal CS-3000 Plus blood cell separator and 24 donors, mean aged (35.4±12.1) years, using Amicus blood cell separator. The number of nucleated cells and CD34+ cells, collection efficiency, collection amount of red blood cell and the time of the hematopoieteic reconstitution of two devices were compared.
RESULTS AND CONCLUSION: There were no significant differences in the total number of nucleated cells and CD34+ cells, collection efficiency, collection amount of red blood cell and the time of the hematopoieteic reconstitution between the two devices. However, the amount of platelet in the stem/progenitor cell products of the Fenwal CS-3000Plus group was obviously greater than that of the Amicus group (P < 0.01). There is no difference in the collection efficiencies of the two blood cell separators in the peripheral blood stem cells/progenitor cell and the effectiveness of transplantation. The Amicus cell separator is better for the donors with thrombocytopenia.

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