中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4295-4298.doi: 10.3969/j.issn.1673-8225.2011.23.026

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

粒细胞集落刺激因子动员2型糖尿病患者外周血CD34+细胞的变化

窦立冬1，崔晓兰2，陈建河1，王立梅1，王意忠1   

  1. 1北京大学航天临床医学院血液内分泌科，北京市   100049
    2中国中医科学院中药所药理室，北京市   100700
  • 收稿日期:2011-03-15 修回日期:2011-04-25 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 王意忠，博士，主任医师，硕士生导师，北京大学航天临床医学院血液内分泌科，北京市 100049 vipwyz@126.com
  • 作者简介:窦立冬★，男，1984年生，河北省人，北京大学在读硕士，主要从事干细胞治疗糖尿病的临床研究。 doulidong999@163.com
  • 基金资助:

    航天科工集团科研基金项目(2009-JKBZ-003)。

Granulocyte colony-stimulating factor mobilizes peripheral blood CD34+ cells in patients with type 2 diabetes mellitus

Dou Li-dong1, Cui Xiao-lan2, Chen Jian-he1, Wang Li-mei1, Wang Yi-zhong1   

  1. 1Department of Hematology and Endocrinology, Aerospace Clinical Medical College of Peking University, Beijing  100049, China
    2Pharmacology Laboratory, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing  100700, China
  • Received:2011-03-15 Revised:2011-04-25 Online:2011-06-04 Published:2011-06-04
  • Contact: Wang Yi-zhong, Doctor, Chief physician, Master’s supervisor, Department of Hematology and Endocrinology, Aerospace Clinical Medical College of Peking University, Beijing 100049, China vipwyz@126.com
  • About author:Dou Li-dong★, Studying for master’s degree, Department of Hematology and Endocrinology, Aerospace Clinical Medical College of Peking University, Beijing 100049, China
  • Supported by:

    Scientific Research Fund of China Aerospace Science and Industry Group, No. 2009-JKBZ-003*

PDF

257

被引次数

2

摘要：

背景：与正常人比较，2型糖尿病患者外周血CD34+细胞含量已有明显下降。
目的：分析2型糖尿病患者外周血动员后CD34+细胞比例的变化。
方法：将234例2型糖尿病患者按病程分为5组(<1年，1~5年，>5~<10年，10~15年和≥15年)，予以粒细胞集落刺激因子动员。动员5 d后,使用流式细胞仪检测外周血CD34+细胞的含量,并利用Person简单相关及多元回归方法分析其与病程、血脂、尿酸的关系。
结果与结论：糖尿病者外周血动员后CD34+细胞水平与三酰甘油相关(r=-0.202，P=0.002)，与载脂蛋白B相关(r=-0.276，P=0.000)，与尿酸相关(r=-0.297，P=0.000)。经统计分析发现，糖尿病者外周血动员后CD34+细胞数随糖尿病病程进展而逐渐下降。

关键词: 粒细胞集落刺激因子, 2型糖尿病, CD34+细胞, 动员, 病程, 相关性

Abstract:

BACKGROUND: The number of mobilized peripheral blood CD34+ cells significantly decreases in type 2 diabetes mellitus patients compared with normal subjects.
OBJECTIVE: To investigate the changes of mobilized peripheral blood CD34+ cells in type 2 diabetes mellitus patients.
METHODS: A total of 234 patients with type 2 diabetes mellitus were divided into five categories according to disease duration, newly diagnosed type 2 diabetes (≤1 year) and diabetes duration (1-5, 5-10, 10-15, ≥15 years). CD34+ cells were mobilized with granulocyte colony-stimulating factor. Five days later, the number of peripheral blood CD34+ cells was detected with flow cytometry. Its relationship with disease duration, blood lipids and uric acid was analyzed by person-related analysis and multiple regression analysis.
RESULTS AND CONCLUSION: The number of mobilized peripheral blood CD34+ cells was associated with the triacylglycerol (r=-0.202, P=0.002), the apoprotein-B (r=-0.276, P=0.000), and the uric acid (r=-0.297, P=0.000). Statistical analysis found that, the number of mobilized peripheral blood CD34+ cells decreased with development of diabetes mellitus.

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