中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4282-4286.doi: 10.3969/j.issn.1673-8225.2011.23.023

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

远志总皂苷干预β-淀粉样蛋白致伤神经干细胞Caspase-3及Par-4的表达

张晓梅，孙光涛，黄作义，吴成吉，戚询中，朱晓峰   

  1. 佳木斯大学神经科学研究所，黑龙江省佳木斯市 154007
  • 收稿日期:2010-12-28 修回日期:2011-02-25 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 朱晓峰，博士，教授，主任医师，博士生导师，佳木斯大学副校长，佳木斯大学神经科学研究所所长，黑龙江省佳木斯市154007 sgtlunwen@163.com
  • 作者简介:张晓梅★，女，1969年生，黑龙江省佳木斯市人，汉族，黑龙江省中医药大学毕业，硕士，教授，主任医师，硕士生导师，主要从事神经干细胞的基础及应用研究。 sgtlunwen@163.com
  • 基金资助:

    国家自然科学基金资助(30971039)：《Mash1通路在NGF诱导神经干细胞向胆碱能神经元定向分化中的调控作用及机制》；黑龙江省自然科学基金资助(D2007-02)：《Noggin基因修饰神经干细胞移植治疗局灶性脑缺血的实验研究》；黑龙江省自然科学基金资助(D200617)：神经干细胞移植治疗缺血性脑血管病及移植环境优化的实验研究。

Effect of tenuigenin on the Caspase-3 and Par-4 expression of neural stem cells induced by beta-amyloid protein

Zhang Xiao-mei, Sun Guang-tao, Huang Zuo-yi, Wu Cheng-ji, Qi Xun-zhong, Zhu Xiao-feng   

  1. Institute of Neuroscience, Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
  • Received:2010-12-28 Revised:2011-02-25 Online:2011-06-04 Published:2011-06-04
  • Contact: Zhu Xiao-feng, Doctor, Professor, Chief physician, Doctoral supervisor, Institute of Neuroscience, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China sgtlunwen@163.com
  • About author:Zhang Xiao-mei★, Master, Professor, Chief physician, Master’s supervisor, Institute of Neuroscience, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China sgtlunwen@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30971039*; the Natural Science Foundation of Heilongjiang Province, No. D2007-02*; the Natural Science Foundation of Heilongjiang Province, No. D200617*

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摘要：

背景：远志总皂苷具有良好的神经保护作用。
目的：分析远志总皂苷对β-淀粉样蛋白致伤海马神经干细胞的保护作用及机制。
方法：自昆明小鼠海马分离培养神经干细胞，取第3代神经干细胞，用含不同质量浓度远志总皂苷与β-淀粉样蛋白致伤体外培养的神经干细胞共孵育。
结果：应用免疫组织化学法检测Caspase-3阳性神经干细胞，与对照组相比，远志总皂苷组Caspase-3阳性细胞率及Par-4的表达明显降低，差异具有显著性意义(P < 0.05)。提示远志总皂苷能够降低β-淀粉样蛋白致伤神经干细胞中Caspase-3及Par-4的表达。

关键词: 远志总皂苷, 神经干细胞, Caspase-3, Par-4, &beta, -淀粉样蛋白

Abstract:

BACKGROUND: The effect of tenuigenin which has a good nerve protection to neural stem cells has not been reported.
OBJECTIVE: To investigate the protective effect and mechanism of tenuigenin for neural stem cells in the hippocampus impaired by β-amyloid protein.
METHODS: The third passage neural stem cells generated from the hippocampi of Kunming mice which injured by β-amyloid protein in vitro were incubated with different concentrations of tenuigenin.
RESULTS AND CONCLUSION: Immunocytochemical technique was used to detect Caspase3-positive neural stem cells. The study revealed that the expression of Par-4 and caspase-3 positive neural stem cells in the tenuigenin group were significantly lower than that in the control group with statistical significance (P < 0.05). Tenuigenin can reduce the expression of Par-4 and Caspase-3 in neural stem cells impaired by β-amyloid protein.

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