中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3559-3562.doi: 10.3969/j.issn.1673-8225.2011.19.033

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

第一鳃弓外胚间充质细胞定向脂肪细胞的分化

侯景秋1，2，闫征斌1，2，杨  旭2   

  1. 1大庆油田总医院口腔科，黑龙江省大庆市 163001
    2佳木斯大学口腔医学院，黑龙江省佳木斯市154004
  • 收稿日期:2010-11-02 修回日期:2010-12-11 出版日期:2011-05-07 发布日期:2011-05-07
  • 通讯作者: 闫征斌，博士，副主任医师，大庆油田总医院口腔科，黑龙江省大庆市 163001，佳木斯大学口腔医学院，黑龙江省佳木斯市 154004 yanzhengbin@163.com
  • 作者简介:侯景秋★，女，1971年生，河北省宁河县人，汉族，2005年四川大学华西口腔医学院毕业，硕士，副主任医师，主要从事口腔颌面部发育及修复重建的研究。

Directed differentiation of ectomesenchymal cells isolated from the first branchial arch into adipocytes

Hou Jing-qiu1, 2, Yan Zheng-bin1, 2, Yang Xu2   

  1. 1Department of Stomatology, Daqing Oil Field General Hospital, Daqing  163001, Heilongjiang Province, China
    2College of Stomatology, Jiamusi University, Jiamusi  154004, Heilongjiang Province, China
  • Received:2010-11-02 Revised:2010-12-11 Online:2011-05-07 Published:2011-05-07
  • Contact: Yan Zheng-bin, Doctor, Associate chief physician, Department of Stomatology, Daqing Oil Field General Hospital, Daqing 163001, Heilongjiang Province, China; College of Stomatology, Jiamusi University, Jiamusi 154004, Heilongjiang Province, China yanzhengbin@163.com
  • About author:Hou Jing-qiu★, Master, Associate chief physician, Department of Stomatology, Daqing Oil Field General Hospital, Daqing 163001, Heilongjiang Province, China; College of Stomatology, Jiamusi University, Jiamusi 154004, Heilongjiang Province, China

PDF

319

摘要：

背景：第一鳃弓作为过渡结构，在牙颌面的发育形成过程中，发挥着重要作用。
目的：观察第一鳃弓外胚间充质细胞自我更新能力，以及体外诱导分化成为脂肪细胞的可能性。
方法：分离培养E9.5 Balb/c胎鼠第一鳃弓外胚间充质细胞，以端粒酶原位杂交和BrdU渗入实验检测外胚间充质细胞的增殖能力。相差显微镜下观察细胞形态变化和生长规律，免疫细胞化学染色鉴定细胞表面标志，cDNA探针原位杂交、免疫荧光化学染色观察细胞增殖能力，油红O染色、RT-PCR产物凝胶电泳观察细胞成脂诱导结果。
结果与结论：端粒酶原位杂交和BrdU摄取实验阳性，诱导后细胞表现出典型的脂肪细胞形态，大部分细胞油红O染色阳性，RT-PCR结果显示诱导后细胞表达了脂蛋白脂酶。提示第一鳃弓外胚间充质细胞具有较高的自我更新能力，并可定向诱导分化成为脂肪细胞。

关键词: 第一鳃弓, 间充质细胞, 诱导, 分化, 脂肪细胞

Abstract:

BACKGROUND: During the dentofacial development, the first branchial arch, as a transitional structure, plays an important role.
OBJECTIVE: To investigate the self-renewal of ectomesenchymal cells (EMCs) isolated from the first branchial arch as well as the feasibility of inducing EMCs into adipocytes.
METHODS: The activity of proliferation and self-renewal of EMCs were detected by nuclei labeled with BrdU and fluorescence in situ hybridization for telomerase. Monolayer cultures of EMCs were passaged 3 times and then transferred to adipogenic media. The level of differentiation was evaluated by histological examination and the expression of tissue-specific genes was analyzed by reverse transcription-polymerase chain reaction technique.
RESULTS AND CONCLUSION: Nuclei labeled with BrdU and fluorescence in situ hybridization for telomerase were positive. After being cultured in adipogenesis-inducing medium, EMCs responded by the accumulation of lipid vacuoles and the gene expression of LPL. The findings indicate that EMCs have a high activity of proliferation and self-renewal, and they are capable of differentiation into adipocytes in vitro.

中图分类号: