中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (18): 3354-3356.doi: 10.3969/j.issn.1673-8225.2011.18.030

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

原发性肝癌组织标本采集、保存数据库及组织库的建立与管理

何国林1，高  毅1，潘明新1，张  健2   

  1. 南方医科大学附属珠江医院，1肝胆二科，2肿瘤科，广东省广州市   510282
  • 收稿日期:2010-11-26 修回日期:2010-12-29 出版日期:2011-04-30 发布日期:2011-04-30
  • 通讯作者: 高毅，博士，主任医师，广东省广州市珠江医院肝胆二科，广东省广州市 510282 gaoyi6164@126.com 并列通讯作者：张健，博士，主任医师，南方医科大学附属珠江医院肿瘤科，广东省广州市 510282 zhangjiantumor@126.com
  • 作者简介:何国林，男，1982年生，广西壮族自治区防城港市人，汉族，2005年南方医科大学毕业，医师，主要从事肝胆外科专业的研究。 dwtou@126.com
  • 基金资助:

    国家“863”项目(2006AA02A141)，课题名称：人源细胞混合型生物人工肝的研制与开发。

Establishment and management of hepatocarcinoma tissue bank and data base

He Guo-lin1, Gao Yi1, Pan Ming-xin1, Zhang Jian2   

  1. 1Second Department of Hepatobiliary Surgery, 2Department of Oncology, Zhujiang Hospital Affiliated to Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2010-11-26 Revised:2010-12-29 Online:2011-04-30 Published:2011-04-30
  • Contact: Gao Yi, Doctor, Chief physician, Second Department of Hepatobiliary Surgery,Zhujiang Hospital Affiliated to Southern Medical University, Guangzhou 510282, Guangdong Province, China gaoyi6164@126.com Correspondence to: Zhang Jian, Doctor, Chief physician, Department of Oncology, Zhujiang Hospital Affiliated to Southern Medical University, Guangzhou 510282, Guangdong Province, China
  • About author:He Guo-lin, Physician, Second Department of Hepatobiliary Surgery, Zhujiang Hospital Affiliated to Southern Medical University, Guangzhou 510282, Guangdong Province, China dwtou@126.com
  • Supported by:

    the National High Technology Research and Development Program of China (863 program), No. 2006AA02A141*

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被引次数

15

摘要：

背景：目前，肝癌的遗传资源并没有得到很好的收集和保护，为了保存广东省宝贵的肝癌家系，南方医科大学附属珠江医院建立了肝癌组织库及数据库。
目的：探讨肝癌组织标本的采集和保存以及组织库的建立和信息化管理方法。
方法：收集手术切除的肿瘤组织、癌旁组织、远端正常组织及患者的血清、血浆以及从肿瘤组织中提取的核酸，分别保存组织块、核酸、血清、血浆、淋巴细胞、干细胞等，液氮或-80 ℃冷冻保存；同时建立一套信息系统用于肿瘤组织库的管理，包括标本存放位置、标本使用、实验结果、综合查询、标本废除管理等。
结果与结论：收集标本45例，其中良性肿瘤5例，恶性肿瘤40例，共350份标本，1年来肿瘤组织库运转良好。建立肝癌组织库及数据库可以对标本进行质量控制及信息化管理，保存少见、罕见标本，达到资源保存和共享的目的。

关键词: 肝癌, 组织库, 数据库, 管理, 信息化

Abstract:

BACKGROUND: Currently, the genetic resource of hepatocarcinoma has not be collected and protected well; therefore, in order to save the proband pedigrees of Guangdong Province, a information-based management of tumor database was established in Zhujiang Hospital Affiliated to Southern Medical University.
OBJECTIVE: To investigate the collection and conservation of tumor tissue specimens, and the approaches to information-based management of tumor database.
METHODS: The tumor tissues, tumor-adjacent tissues, distal normal tissues removed from surgery and patient serum and extracted nucleic acids from tumor tissues were collected. Specimens of the tissue fragments, nucleic acids, serum, plasma, lymphocyte and stem cells, etc. were respectively conserved in liquid nitrogen or -80 ℃ refrigerator. Meanwhile, a database of information management of tumor tissue bank was established, including positions of sample placement, specimen use, laboratory results, comprehensive query, management of specimen disposal, etc.
RESULTS AND CONCLUSION: Specimens of 45 cases, including relevant specimens of 5 cases of benign tumors and 40 cases of malignant tumors, were collected. The tumor tissue data base was established and managed according to the above-mentioned methods, which functioned with good results in the past 1 year. The establishment of tumor tissue database enables the quantity control of the specimens and information-based management, as well as conservation of infrequent specimens and resource share.

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