中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (18): 3293-3297.doi: 10.3969/j.issn.1673-8225.2011.18.016

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

深低温冻储同种异体气管移植并骨髓间充质干细胞移植后气管上皮细胞血管内皮生长因子的表达

韩  云1，纪子钊1，蓝  妮2，庞超见2，佟晓杰2，石文君1   

  1. 1中国医科大学附属盛京医院胸外科，辽宁省沈阳市   110004
    2中国医科大学基础医学院解剖教研室，辽宁省沈阳市   110001
  • 收稿日期:2010-10-26 修回日期:2010-12-03 出版日期:2011-04-30 发布日期:2011-04-30
  • 通讯作者: 韩云☆，男，1971年生，辽宁省沈阳市人，汉族，2010年中国医科大学毕业，博士，主治医师，主要从事气管移植的组织工程学研究。 ryan.hanyun@ gmail.com
  • 作者简介:韩云☆，男，1971年生，辽宁省沈阳市人，汉族，2010年中国医科大学毕业，博士，主治医师，主要从事气管移植的组织工程学研究。 ryan.hanyun@ gmail.com
  • 基金资助:

    本课题受辽宁省教育厅科学技术研究项目(L2010606)资助。

Effect of bone marrow derived mesenchymal stem cells transplantation combined with cryopreservation on trachea allograft epithelium vascular endothelial growth factor levels

Han Yun1, Ji Zi-zhao1, Lan Ni2, Pang Chao-jian2, Tong Xiao-jie2, Shi Wen-jun1   

  1. 1Department of Thoracic Surgery, China Medical University Affiliated Shengjing Hospital, Shenyang  110004, Liaoning Province, China
    2Department of Anatomy, College of Basic Medicine, China Medical University, Shenyang  110001, Liaoning Province, China
  • Received:2010-10-26 Revised:2010-12-03 Online:2011-04-30 Published:2011-04-30
  • Contact: Han Yun☆, M.D, Ph.D., Attending surgeon, Department of Thoracic Surgery, China Medical University Affiliated Shengjing Hospital, Shenyang 110004, Liaoning Province, China ryan.hanyun@gmail.com
  • About author:Han Yun☆, M.D, Ph.D., Attending surgeon, Department of Thoracic Surgery, China Medical University Affiliated Shengjing Hospital, Shenyang 110004, Liaoning Province, China ryan.hanyun@gmail.com
  • Supported by:

    Science and Technology Research Program of Liaoning Educational Committee, No. L2010606*

PDF

253

被引次数

2

摘要：

背景：深低温冷冻后的组织可以保持其原有的活力和组织完整性，并能消减抗原性。
目的：静脉移植骨髓间充质干细胞合并深低温处理供体气管后进行同种异体气管移植，观察骨髓间充质干细胞在促进上皮再生和再血管化后的作用。
方法：用深低温冻储2 周和6 周的气管进行Wistar大鼠同种异体气管移植后，用PKH-26标记的3~5代骨髓间充质干细胞经鼠尾静脉移植入受体内，等量的磷酸盐缓冲液注射作为实验对照。观察供体气管的组织学和血管内皮生长因子蛋白表达。
结果和结论：骨髓间充质干细胞移植合并深低温冻储后的移植气管结构完整，软骨无变性坏死；深低温6 周的移植气管上皮再生情况优于2 周。骨髓间充质干细胞移植组的上皮细胞表达血管内皮生长因子水平高于磷酸盐缓冲液对照组。结果提示，骨髓间充质干细胞移植合并深低温冻储6 周后移植气管存活期好于深低温冻储2周；骨髓间充质干细胞能够促进移植物周围新生血管的增加，从而促进气管损伤的修复。

关键词: 气管移植, 骨髓间充质干细胞, 同种异体, 深低温冻储, 血管内皮生长因子

Abstract:

BACKGROUND: Cryopreserved tissues can maintain their vitality and integrity, and reduce the antigenicity.
OBJECTIVE: Using intravenous transplantation of bone marrow derived mesenchymal stem cells (BMSCs) combined with cryopreserved allograft, to investigate the role of BMSCs on enhancing the implant survival and revascularization by measuring vascular endothelial growth factor (VEGF) levels.
METHODS: After transplanted with cryopreserved 2 weeks and 6 weeks allocraft, PKH-26 labeled 3-5 passage BMSCs were injected into the recipient rats via the tail vein. Rats in the control groups were injected with the same amount of phosphate buffer solution (PBS). We observed the histology of the transplanted trachea and measured the VEGF protein levels in the epithelium to evaluate the role of BMSCs transplantation on the epithelium revascularization.
RESULTS AND CONCLUSION: Rats from BMSCs injection group survived a long period. Histological observation showed that the tracheal lumen was covered by psudo-striated ciliated columnar epithelium. VEGF protein levels in the BMSCs injection group were higher than PBS group. BMSCs may exert their reparation function via enhancing epithelium VEGF expression and revascularization.

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