中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6729-6732.doi: 10.3969/j.issn.1673-8225.2010.36.018

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

异体骨髓间充质干细胞移植治疗大鼠脊髓损伤

阮  智，黄  慧，孙建华，史晨辉，董金波，王永明   

  1. 新疆石河子大学医学院第一附属医院骨一科，新疆维吾尔自治区石河子市  832008
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 通讯作者: 史晨辉，硕士，主任医师，教授，副院长，新疆石河子大学医学院第一附属医院，新疆维吾尔自治区石河子市 832008 sch7890@yahoo.com.cn
  • 作者简介:阮智★，男，1973年生，湖北省武汉市人，汉族，石河子大学在读硕士，主治医师，主要从事骨外科方面的研究。 ruanzhi113@sina.com

Allogeneic bone marrow mesenchymal stem cells transplantation for spinal cord injury in rats

Ruan Zhi, Huang Hui, Sun Jian-hua, Shi Chen-hui, Dong Jin-bo, Wang Yong-ming   

  1. First Department of Orthopedics, First Affiliated Hospital, Medical College, Shihezi University, Shihezi  832008, Xinjiang Uygur Autonomous Region, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Shi Chen-hui, Master, Chief physician, Professor, First Department of Orthopedics, First Affiliated Hospital, Medical College, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China sch7890@yahoo. com.cn
  • About author:Ruan Zhi★, Studying for master’s degree, Attending physician, First Department of Orthopedics, First Affiliated Hospital, Medical College, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China ruanzhi113@sina.com

PDF

499

被引次数

38

摘要：

背景：脊髓损伤的修复目前尚无良好的治疗手段，细胞移植能促进神经轴突再生及脊髓功能恢复，为治疗脊髓损伤提供了可能，但因脊髓损伤模型及移植方式不同，其治疗效果并不相同。
目的：验证异体骨髓间充质干细胞移植对大鼠脊髓损伤的治疗作用。
方法：全骨髓贴壁法分离大鼠骨髓间充质干细胞。健康SD大鼠随机分为3组，细胞移植组、对照组和假手术组。细胞移植组和对照组采用改良Allen重物打击法制造大鼠脊髓损伤模型，假手术组仅暴露脊髓。术后4周，每周进行运动功能评分，ELISA检测脊髓损伤组织中脑源性神经营养因子、神经生长因子表达；免疫荧光染色检测脊髓组织中NF200和胶质纤维酸性蛋白表达。
结果与结论：与对照组比较，细胞移植组大鼠运动功能明显改善，脊髓组织中脑源性神经营养因子、神经生长因子蛋白含量明显增高(P < 0.05)；移植组大鼠脊髓囊腔较小，NF200表达明显增加，胶质纤维酸性蛋白表达减少。提示异体骨髓间充质干细胞移植能增加损伤脊髓神经生长因子含量，抑制胶质瘢痕形成，促进神经轴突再生，改善大鼠脊髓损伤后运动功能恢复。

关键词: 脊髓损伤, 骨髓间充质干细胞, 神经生长因子, 异体移植, 轴突再生

Abstract:

BACKGROUND: There is no good therapeutic tool for repairing spinal cord injury (SCI). Cell transplantation can contribute to axonal regeneration and the recovery of spinal cord function, and provide possibility for treating SCI. However, the SCI models and transplanted manners are various, and the therapeutic efficiency is different.
OBJECTIVE: To investigate the therapeutic effects of allogeneic bone marrow mesenchymal stem cells (BMSCs) transplantation on rat SCI.
METHODS: BMSCs were isolated from rats by the whole bone marrow method. Healthy Sprague Dawley rats were randomly assigned to cell transplantation, control and sham surgery groups. In the cell transplantation and control groups, rat SCI models were established by the modified Allen’s method. In the sham surgery group, the spinal cord was exposed only. At 4 weeks following surgery, locomotive function of SCI rats was graded weekly. Enzyme linked immunosorbent assay was used to detect expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in injured spinal cord. Immunofluorescent staining was employed to detect the expression of NF200 and glial fibrillary acidic protein (GFAP). 
RESULTS AND CONCLUSION: Compared with control group, movement function was significantly improved, and BDNF and NGF protein contents were significantly increased in the spinal cord in the cell transplantation group (P < 0.05). The spinal cord capsular space was small; NF200 expression was significantly increased; GFAP expression was reduced in the rats from the transplantation group. Results indicate that allogeneic BMSCs transplantation could increase NGF levels, inhibit the glial scar formation and promote axonal regeneration and improve functional recovery in rats after SCI.

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