中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6721-6723.doi: 10.3969/j.issn.1673-8225.2010.36.016

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

同种异体骨髓间充质干细胞穴位移植：急性心肌梗死后血管新生及相关细胞因子的变化*

张  哲，李  峥，杨关林，陈  岩   

  1. 辽宁中医药大学附属医院，辽宁省沈阳市  110032
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 作者简介:张哲☆，女，1976年生，汉族， 2009年辽宁中医药大学毕业，博士，副主任医师，主要从事中西医结合临床心血管研究。 goldkey_ren@sohu.com
  • 基金资助:

    辽宁省教育厅科学技术研究项目（2004D281）。

Acupoint transplantation of allogenic bone marrow mesenchymal stem cells: Angiogenesis and relevant cytokine changes following acute myocardial infarction

Zhang Zhe, Li Zheng, Yang Guan-lin, Chen Yan   

  1. Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang  110032, Liaoning Province, China
  • Online:2010-09-03 Published:2010-09-03
  • About author:Zhang Zhe☆, Doctor, Associate chief physician, Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China goldkey_ren@sohu. com
  • Supported by:

    the Science and Technology Research Program of Education Department of Liaoning Province, No. 2004D281

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摘要：

背景：骨髓间充质干细胞移植途径主要包括外周静脉注射移植、冠状动脉移植以及心肌内注射移植，但存在迁移缺少靶向性，价格高昂等局限性。
目的：观察同种异体骨髓间充质干细胞穴位移植兔急性心肌梗死后梗死区血管新生及相关细胞因子的变化。
方法：提取兔骨髓进行骨髓间充质干细胞的分离、培养、纯化、扩增、鉴定，并标记。将55只兔随机分为正常组与手术组。手术组急性心肌梗死后1周，再随机分为3组：穴位注射干细胞组、模型对照组、穴位注射生理盐水组。5周后，取心肌组织进行免疫组织化学BrdU染色、心肌特异性肌钙蛋白T免疫组织化学染色检测；免疫组织化学法检测梗死心肌组织血小板内皮细胞黏附因子1水平；免疫组织化学法、夹心酶联免疫法、RT-PCR法、Western-Blot法检测血管内皮细胞生长因子及免疫组织化学法检测碱性成纤维细胞生长因子。
结果与结论：穴位注射的骨髓间充质干细胞可迁移至梗死区，并可以在梗死区分化为心肌样细胞。与模型对照组及穴位注射生理盐水组比较，穴位注射骨髓间充质干细胞可使梗死区及周围组织血小板内皮细胞黏附因子1表达上调，诱导梗死区及周围组织血管新生，其机制是刺激心肌组织中碱性成纤维细胞生长因子表达上调。穴位注射骨髓间充质干细胞组梗死心肌组织中和血清中血管内皮细胞生长因子表达均无上调趋势，因此推测，血管新生由碱性成纤维细胞生长因子及未知因素诱导。

关键词: 穴位注射, 骨髓间充质干细胞, 心肌梗死, 血管新生, 细胞因子

Abstract:

BACKGROUND: The bone marrow mesenchymal stem cell (BMSC) transplantation ways mainly contain peripheral vein injection transplantation, coronary artery transplantation and intramyocardial injection transplantation, but there are limitations such as lack of target and high price.
OBJECTIVE: To observe effects of allogenic BMSC transplantation on angiogenesis and changes in related cytokines in the infarct region following rabbit acute myocardial infarction.
METHODS: The rabbit bone marrow was extracted to isolate, culture, purify, amplify, identify and label BMSCs. A total of 55 rabbits were randomly assigned to normal and operation groups. In the operation group, at 1 week following acute myocardial infarction, the rabbits were randomly divided into three groups: acupoint injection stem cell group, model control group and acupoint injection saline group. Five weeks later, myocardial tissue was obtained for immunohistochemistry BrdU staining and cardiac specific troponin T immunohistochemistry staining. Immunohistochemistry was used to detect platelet/endothelial cell adhesion molecule-1 levels in infarct myocardial tissue. Immunohistochemistry, sandwich enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction and Western blot assay were utilized to determine vascular endothelial cell growth factor. Immunohistochemistry was employed to detect basic fibroblast growth factor.
RESULTS AND CONCLUSION: BMSCs by acupoint injection could migrate towards infarct region, and differentiate into cardiac-like cells. Compared with model control group and acupoint injection saline group, platelet/endothelial cell adhesion molecule-1 expression upregulated in and surrounding the infarct tissue in the acupoint injection stem cell group. The upregulation of platelet/endothelial cell adhesion molecule-1 expression induced angiogenesis in and surrounding the infarct region. Its mechanism is to stimulate upregulation of basic fibroblast growth factor expression in myocardial tissue. Vascular endothelial cell growth factor expression did not exhibit upregulatory tendency in the infarct myocardial tissue and serum in the acupoint injection stem cell group. Thus, angiogenesis is induced by basic fibroblast growth factor and unknown factors.

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