中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6699-6702.doi: 10.3969/j.issn.1673-8225.2010.36.011

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

低氧预适应刺激脑海马区内源性神经干细胞的增殖

张颜波，杨明峰，孙保亮，牛敬忠   

  1. 泰山医学院附属医院神经内科，山东省泰安市 271000
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 作者简介:张颜波★，男，1978年生，山东省枣庄市人，汉族，2004年首都医科大学毕业，硕士，主治医师，讲师，主要从事低氧预适应、内脏炎症痛及其机制方面的研究。 bbnnbn@163. com
  • 基金资助:

    山东省医药卫生发展计划(2009HZ096)，课题名称“低氧预适应脑保护机制对脑梗死治疗作用的应用研究”；山东省教育厅科研发展计划(J08LG71)，课题名称“低氧预适应小鼠脑匀浆提取液对缺氧复氧培养海马神经元细胞的保护作用及机制研究”。

Hypoxic preconditioning stimulates proliferation of endogenous neural stem cells in brain hippocampus

Zhang Yan-bo, Yang Ming-feng, Sun Bao-liang, Niu Jing-zhong   

  1. Department of Neurology, Affiliated Hospital of Taishan Medical College, Taian 271000, Shandong Province, China
  • Online:2010-09-03 Published:2010-09-03
  • About author:Zhang Yan-bo★, Master, Attending physician, Lecturer, Department of Neurology, Affiliated Hospital of Taishan Medical College, Taian 271000, Shandong Province, China bbnnbn@163.com
  • Supported by:

    the Medical Science Development Project of Shandong Province, No. 2009HZ096*; the Scientific Research Development Project of Shandong Province, No. J08LG71*

PDF

457

被引次数

24

摘要：

背景：缺血/缺氧/低氧等刺激均能导致内源性神经干细胞的增殖和分化，起到脑组织修复作用，但低氧预适应能否影响内源性神经干细胞的增殖尚不清楚。
目的：探讨低氧预适应对小鼠脑海马区内源性神经干细胞增殖的影响。
方法：清洁级Balb/c近交系小鼠随机分为3组，低氧对照组小鼠放入广口瓶内，立即用橡皮塞封紧，以动物出现第1次喘呼吸为低氧耐受极限的标志，完成低氧暴露1次；低氧预适应组小鼠按此法重复操作4次；正常对照组小鼠不进行低氧暴露。通过免疫荧光和激光共聚焦显微镜等技术，测定海马BrdU阳性细胞数和荧光强度。
结果与结论：与低氧对照组比较，低氧预适应组小鼠耐受时间显著延长(P < 0.01)。正常对照组海马区BrdU标记的内源性神经干细胞荧光强度微弱，海马区可见少量BrdU阳性细胞；低氧对照组、低氧预适应组荧光强度及BrdU阳性细胞数均明显增加(P < 0.01)，且低氧预适应组增加幅度大于低氧对照组(P < 0.01)。证实在低氧预适应过程中，海马区内源性神经干细胞明显增殖，可能参与低氧预适应脑保护机制。

关键词: 低氧预适应, BrdU, 海马, 小鼠, 脑保护, 神经干细胞

Abstract:

BACKGROUND: Ischemic, hypoxic, hypoxia and other stimuli can lead to endogenous neural stem cell proliferation and differentiation, which play a role in brain repair, but it is still not clear whether hypoxic preconditioning can affect the proliferation of endogenous neural stem cells.
OBJECTIVE: To explore the proliferation of endogenous neural stem cells in hippocampus of hypoxic preconditioning mice.
METHODS: Balb/c clean mice were randomly divided into three groups. Mice in the hypoxia control group were placed in a wide-mouthed bottle, blocked by rubber stopper. Animals with the first asthmoid respiration marked tolerance limit of hypoxia, and once hypoxia exposure was completed. Above-mentioned procedures were repeated four times in the hypoxic preconditioning group. Mice in the normal control group were not exposed to hypoxia. The number and fluorescence intensity of BrdU-labeled cells were counted and observed by immunofluorescence and confocal laser scanning microscope.
RESULTS AND CONCLUSION: Compared with hypoxia control group, the tolerance time was significantly longer in the hypoxic preconditioning group (P < 0.01). Fluorescence intensity of BrdU-labeled endogenous neural stem cells was faint, and a few BrdU-positive cells were visible in the hippocampus in the normal control group. The fluorescence intensity and BrdU-positive cell number were significantly increased in the hypoxia control and hypoxic preconditioning groups (P < 0.01). The increased range was greater in the hypoxic preconditioning group compared with the hypoxia control group (P < 0.01). Results suggest that proliferation of endogenous neural stem cells is seen obviously in hippocampus of hypoxic preconditioning mice, which is perhaps involved in cerebral protective mechanism of hypoxic preconditioning.

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