中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6667-6671.doi: 10.3969/j.issn.1673-8225.2010.36.004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞诱导心脏成纤维细胞向肌纤维母细胞转化：不同时期心肌梗死区域的最佳微环境

林楚伟，周胜华，刘启明，杜优优，姚丽群，张  良，柴湘平   

  1. 中南大学湘雅二医院心内科，湖南省长沙市  410000
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 作者简介:林楚伟☆，男，1982年生，湖南省浏阳市人，汉族，中南大学在读博士，主要从事心肌梗死干细胞治疗的研究。 linchuwei@ yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(30871053)，湖南省自然科学基金资助项目(10JJ3064)。

Bone marrow mesenchymal stem cells-induced transformation of cardiac fibroblasts into myofibroblasts: Optimal microenvironment of myocardial infarction at different stages

Lin Chu-wei, Zhou Sheng-hua, Liu Qi-ming, Du You-you, Yao Li-qun, Zhang Liang, Cai Xiang-ping   

  1. Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha  410000, Hunan Province, China
  • Online:2010-09-03 Published:2010-09-03
  • About author:Lin Chu-wei☆, Studying for doctorate, Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha 410000, Hunan Province, China linchuwei@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30871053*; the Natural Science Foundation of Hunan Province, No. 10JJ3064*

PDF

343

摘要：

背景：骨髓间充质干细胞移植能够有效改善心肌梗死后的心功能，其作用机制可能与移植部位大量肌纤维母细胞的聚集有关，但至今仍缺少证据来证实聚集的肌纤维母细胞是由骨髓间充质干细胞还是由成纤维细胞转化而来？ 
目的：观察不同时期心肌梗死微环境对大鼠骨髓间充质干细胞诱导心脏成纤维细胞向肌纤维母细胞转化的影响。
方法：将培养组分为3组：大鼠骨髓间充质干细胞、心脏成纤维细胞单培养组，以及两种细胞共培养组，加入心肌梗死后不同时期心肌匀浆干预7~28 d，并设立不加心肌匀浆的空白对照组，运用免疫细胞化学染色方法，检测不同干预组间平滑肌肌动蛋白(α-SMA)表达及成纤维细胞转化为肌纤维母细胞数量间的差异。
结果与结论：加用心肌梗死第14天梗死周边区域心肌匀浆干预的共培养组，检测到心脏成纤维细胞转化为肌纤维母细胞的阳性率最高。提示心肌梗死后微环境下，骨髓间充质干细胞能诱导诱导心脏成纤维细胞转化为肌纤维母细胞，而心肌梗死后14 d的心肌梗死微环境是骨髓间充质干细胞诱导心脏成纤维细胞转化为肌纤维母细胞的最佳微环境。

关键词: 成纤维细胞, 骨髓间充质干细胞, 肌纤维母细胞, 心肌梗死, 微环境

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) can effectively improve heart function following myocardial infarction. Its action mechanism is associated with aggregation of a large number of myofibroblasts in the transplant region, but few reports have verified that gathered myofibroblasts are transformed from BMSCs or from fibroblasts.
OBJECTIVE: To explore the impact of microenvironment of myocardial infarction in different periods in rat BMSCs inducing cardiac fibroblasts into myofibroblasts.
METHODS: The culture group was assigned to three groups: rat BMSCs group, cardiac fibroblasts group and combination group. Myocardial homogenate was added at different times after myocardial infarction for 7-28 days. A blank control group was set, without myocardial homogenate. Immunocytochemical staining was used to determine the differences of α-smooth muscle actin (α-SMA) expression and the number of fibroblasts into myofibroblasts between the two groups.
RESULTS AND CONCLUSION: In the combination group, the positive rate of cardiac fibroblasts into myofibroblasts is highest, when BMSCs and cardiac fibroblasts were cultured together and intervened by myocardial homogenate on day 14 after myocardial infarction. Results suggest that in the microenvironment of post-myocardial infarction, BMSCs can induce cardiac fibroblasts into myofibroblasts, while the microenvironment of post-myocardial infarction on day 14 is the best one.

中图分类号: