中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (35): 6567-6572.doi: 10.3969/j.issn.1673-8225.2010.35.027

• 骨与关节图像与影像 bone and joint imaging • 上一篇    下一篇

周围神经功能束二维全景图像配准定位标志点的获取

张  毅1，卢  昊2，何彩风3，戚  剑4，罗  鹏4，刘小林4   

  1. 中山大学附属第一医院，1整形修复外科，3骨科研究所，4显微创伤骨科，广东省广州市  510080；2解放军第三军医大学整形外科，重庆市  400038
  • 出版日期:2010-08-27 发布日期:2010-08-27
  • 通讯作者: 刘小林，教授，博士生导师，中山大学附属第一医院显微创伤骨科，广东省广州市 510080 gzxiaolinliu@hotmail.com
  • 作者简介:张毅☆，男，1971年生，湖南省城步县人，汉族，2009年中山大学毕业，博士，主治医师，主要从事显微整形修复、周围神经外科。 Doczy2006@126.com
  • 基金资助:

    国家自然科学基金资助项目“四肢周围神经损伤缺损显微修复的数字化模拟系统研究”(30571913)，广东省自然科学基金“周围神经功能束三维数字模拟系统构建的关键技术研究”(9151008901000006)。

Acquisition of complete locating points on 2-D Panoramagram for registration of functional fascicular groups of the peripheral nerve

Zhang Yi1, Lu Hao2, He Cai-feng3, Qi Jian4, Luo Peng4, Liu Xiao-lin4   

  1. 1 Department of Plastic and Reconstructive Surgery, 3 Orthopedic Institute, 4Department of Microsurgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China; 2 Division of Plastic Surgery, Third Military Medical University, Chongqing  400038, China
  • Online:2010-08-27 Published:2010-08-27
  • Contact: Liu Xiao-lin, Professor, Doctoral supervisor, Department of Microsurgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China gzxiaolinliu@hotmail.com
  • About author:Zhang Yi☆, Doctor, Attending physician, Department of Plastic and Reconstructive Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China Doczy2006@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30571913*; the Natural Science Foundation of Guangdong Province, No. 91510089010000 06*

PDF

413

被引次数

4

摘要：

背景：实现二维图像的计算机自动配准是三维重建的关键步骤，目前针对神经断面二维图像自动配准软件开发要求图像具备4个完整定位标志点，此前二维全景图像完整定位标志点的获取这一关键技术问题未获得有效解决。
目的：了解周围神经功能束三维可视化重建中用于配准神经断面二维图像定位标志点获取的关键技术。
方法：① 取自愿捐献新鲜成人截肢坐骨神经标本，以4根成年女性头发作为标志线，横断面连续冰冻切片100张，片厚10 µm，分别以普通玻片及防脱多聚赖氨酸涂层硅化玻片贴片进行AchE和苏木精-伊红染色，OLYMPUS-Z61型数码全景成像仪一次性获取全景图像，观察比较不同玻片及不同染色方法下头发标志线的贴片情况。②新鲜成人尸体上臂尺神经标本切片，于AChE 染色前、后二次成像获取同一切片的两张全景图像，通过Photoshop CS2图层叠加进行图像处理，获取含4个完整定位标志点的神经断面二维全景图像。
结果与结论：①在染色后各组人发标志点脱失均较严重，各组间人发贴片数量级差异无显著性意义(P > 0.05)，100张图像的人发完整贴片率为1%，总体黏附率为22.5%，人发作为周围神经超薄冰冻组织切片标志点染色后完整贴片率很低，不同的染色方法和不同黏附性能的玻片对人发染色后的贴片率影响没有明显差别；染色前图像完整贴片率达98.0%，总体黏附率为99.5%，染色前人发标志点完整贴片率很高，不同曝光情况处理及染色前封固处理均显示标志点定位清晰。染色前后人发标志点数量差异有显著性意义(P < 0.01)。②利用二次成像技术和Photoshop图层处理技术处理同一张切片染色前后的图像，图像轮廓吻合度良好，合成图像获得的标志点定位精确，操作简单快捷，可获取完整定位标志点用于配准。

关键词: 周围神经, 功能束, 二维全景图像, 定位标志点, 数字化医学

Abstract:

BACKGROUND: Computer automatic registration of 2-D images is a key step for 3-D reconstruction. The software development of nerve cross section requires 4 complete locating points.
OBJECTIVE: To explore the key technology to capture the complete locating points on 2-D Panoramagram for registration of the functional fascicular 3-D visualization of peripheral nerve.
METHODS: A segment donating fresh adult sciatic nerve with 4 piece of woman’s hairs fixed around the nerve was serially horizontally sliced into 100 slices, 10 µm thick. According to the different adhesion glass (polylysine coated silicification glass and ordinary glass) and the different ways of staining (hematoxylin-eosin and Karnovsky-Roots AChE staining). The 2-D panorama images of nerve section slice before and after staining were obtained by OLYMPUS-Z61 micro digital photography device. The counts of hair locating pots of each image were compared between pro and post staining. The slices of a segment donating fresh adult ulnar nerve of upper arm were stained with AchE, 2-D panorama images of the same slice were captured before and after AchE staining. Using the layer processing technique of Photoshop CS, the recomposition images including complete 4 location pots were obtained.
RESULTS AND CONCLUSION: The missing of hair locating pots was serious after staining. The remaining hair location points on the glass had no significant differences among the groups (P > 0.05); the rate of complete adhesion of 100 image was 1%, with a total adhesion rate of 22.5%. The hair locating pots adhesion rate of frozen slices of peripheral nerve is low after staining. Staining did not affect adhesion rate: the rate of complete adhesion and the rate of total adhesion before staining were 98.0% and 99.50% respectively. The position of the pots before staining was accurate. The outline of the merging 2-D panorama images has fine goodness of fit，the 4 locating points of is accurate, the whole procedure is simple and fast. Based on the two-time imaging technique of the same slice before and after AChE staining and the photoshop layer processing technique, the 2-D panorama images with complete locating points can be obtained.

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