中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (35): 6496-6499.doi: 10.3969/j.issn.1673-8225.2010.35.009

• 人工假体 artificial prosthesis • 上一篇    下一篇

人工关节无菌性松动界膜中的溶骨因子

刘天盛，王宇强，王景贵，苏  彬   

  1. 武警医学院附属医院骨科，天津市    300162
  • 出版日期:2010-08-27 发布日期:2010-08-27
  • 作者简介:刘天盛★，男，1973年生，天津市人，汉族，1997年天津医科大学毕业，硕士，副主任医师，主要从事骨关节疾病的研究。 landslands2003@sina.com
  • 基金资助:

    武警医学院科研基金资助项目(wjyxy2006-15)，课题名称人工关节无菌性松动界膜中溶骨因子的研究。

Osteolysis cytokine in limiting membrane of joint prosthesis aseptic loosening

Liu Tian-sheng, Wang Yu-qiang, Wang Jing-gui, Su Bin   

  1. Department of Orthopaedics, Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin  300162, China
  • Online:2010-08-27 Published:2010-08-27
  • About author:Liu Tian-sheng★, Master, Associate chief physician, Department of Orthopaedics, Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China landslands2003@sina.com
  • Supported by:

    the Scientific Research Foundation Program of Medical College of Chinese People’s Armed Police Force, No. wjyxy2006-15*

PDF

304

被引次数

7

摘要：

背景：研究证实人工关节无菌性松动是一个溶骨的过程，溶骨因子在其中具有重要作用，但目前只有肿瘤坏死因子、前列腺素E2的相关报道，对于其他溶骨因子在这一过程中的作用尚未见相关研究报道。
目的：检测人工关节无菌性松动患者界膜组织中溶骨因子的表达。
方法：将2006-03/2008-12武警医学院附属医院及协作医院收治的发生人工关节无菌性松动并行关节翻修的54例患者设为观察组，翻修术中收集界膜。同时收集武警医学院内固定取出术患者56例设为对照组，内固定取出术中收集内固定物与骨之间组织。两组标本均以免疫组化方法检测白细胞介素1、白细胞介素6、白细胞介素11、单核细胞集落刺激因子等促进溶骨细胞因子的染色指数，同时以反转录-聚合酶链反应方法检测细胞因子mRNA表达情况。
结果与结论：白细胞介素1、白细胞介素6、白细胞介素11、单核细胞集落刺激因子在人工关节无菌性松动患者界膜组织中的表达水平明显高于对照组(P < 0.05)。提示白细胞介素1、白细胞介素6、白细胞介素11、单核细胞集落刺激因子等溶骨因子在人工关节无菌性松动病理过程中发挥了重要作用，如能阻断其病理过程将有效减缓和预防人工关节无菌性松动的发生。

关键词: 人工关节, 无菌性松动, 溶骨因子, 反转录-聚合酶链反应, 免疫组化

Abstract:

BACKGROUND: Artificial joint aseptic loosening is a process of osteolysis, in which osteolysis cytokines play important roles. However, only tumor necrosis factor and prostaglandin E2 have been reported.
OBJECTIVE: To detect the osteolysis cytokine expression in limiting membrane of joint prosthesis aseptic loosening.
METHODS: A total of 54 patients who had joint prosthesis aseptic loosening and underwent revision in Affiliated Hospital of Medical College of Chinese People’s Armed Police Force and Union Hospital from March 2006 to December 2008 were served as observation group and to the limiting membrane in the operation was collected. In addition, 56 patients with fixator removal were served as control group, and the scar tissues were collected. Interleukin-1 (IL-1), IL-6, IL-11, and macrophage colony-stimulating factor (M-CSF) were detected by immunohistochemisty method, and cytokine mRNA expression was detected by RT-PCR.
RESULTS AND CONCLUSION: IL-1, IL-6, IL-11, and M-CSF expression was greater in limiting membrane of joint prosthesis loosening patients than the control group (P < 0.05). Results suggested that IL-1, IL-6, IL-11, and M-CSF play an important role in the joint prosthesis loosening, and blocking this pathological process may effectively delay and prevent joint aseptic loosening.

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