中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (34): 6347-6350.doi: 10.3969/j.issn.1673-8225.2010.34.018

• 药物控释材料 drug delivery materials • 上一篇    下一篇

海藻酸钙凝胶栓塞治疗兔VX2肝种植瘤

赵慧敏1，张祥林2   

  1. 1辽宁医学院，辽宁省锦州市  121001；2 辽宁医学院第一附属医院，辽宁省锦州市121001
  • 出版日期:2010-08-20 发布日期:2010-08-20
  • 通讯作者: 张祥林，主任医师，辽宁医学院第一附属医院，辽宁省锦州市 121001 zhangxianglinjr@126.com
  • 作者简介:赵慧敏★，男，1972年生，河南省郑州市人，汉族，辽宁医学院在读硕士，主治医师，主要从事肿瘤介入治疗研究。 zhaohuimin505@126.com

Calcium alginate gel for treating VX2 transplanted liver tumor of rabbits

Zhao Hui-min1, Zhang Xiang-lin2   

  1. 1 Liaoning Medical College, Jinzhou   121001, Liaoning Province, China; 2 The First Affiliated Hospital, Liaoning Medical College, Jinzhou   121001, Liaoning Province, China
  • Online:2010-08-20 Published:2010-08-20
  • Contact: Zhang Xiang-lin, Chief physician, The First Affiliated Hospital, Liaoning Medical College, Jinzhou 121001, Liaoning Province, China zhangxianglinjr@126.com
  • About author:Zhao Hui-min★, Studying for master’s degree, Attending physician, Liaoning Medical College, Jinzhou 121001, Liaoning Province, China zhaohuimin505@126.com

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407

被引次数

1

摘要：

背景：液体海藻酸钙凝胶是最近出现的一种新型栓塞材料，具有良好的生物相容性，可经微导管输送，不易形成异位栓塞等特点，在动静脉畸形和动脉瘤动物实验中效果良好。
目的：为了寻找更安全有效的肝癌栓塞材料，创新性使用液体海藻酸钙凝胶肝动脉栓塞兔VX2肝种植瘤。
方法：采用改良开腹接种法建立VX2肝种植瘤模型。以数字表法随机分为对照组、实验组。直视下细针穿刺肝动脉造影，对照组仅行肝动脉造影，实验组造影后使用液体海藻酸钙凝胶栓塞治疗。于栓塞治疗前、治疗后7d行肝脏CT扫描，多平面重建测量肿瘤大小。栓塞治疗7d后取肿瘤行病理组织学检查，评估海藻酸钙凝胶栓塞对肿瘤和正常肝组织的影响。
结果与结论：使用液体海藻酸钙凝胶栓塞后造影显示肿瘤的供血动脉中断，闭塞。实验组兔肝肿瘤生长率明显低于对照组  (P < 0.05)。对照组肿瘤坏死以轻度坏死为主；实验组肿瘤坏死以重度坏死为主，肝可见多发灶状坏死。两组间坏死程度差异有显著性意义(P < 0.05)。实验组甲苯胺蓝染色可见肝动脉分支、叶间小动脉，入肝微动脉完全闭塞，肝窦内有少量海藻酸钙凝胶。结果说明海藻酸钙凝胶可在肝肿瘤血管门部至入肝微动脉间形成广泛栓塞，阻断肿瘤的血供，抑制肿瘤的生长。

关键词: 海藻酸钙凝胶, VX2肝种植瘤, 动脉栓塞, 动物实验, 生物材料与药物控释

Abstract:

BACKGROUND: Liquid calcium alginate gel is a relatively new embolic material, it has good biocompatibility and can be piped through micro-catheter with fewer heterotopic embolization, results are satisfactory in animal experiments of arteriovenous malformations and aneurysm.
OBJECTIVE: To research a safer and more effective agent for liver tumor embolism, we innovatively use calcium alginate gel transcatheter arterial embolization for VX2 rabbit liver tumor.
METHODS: VX2 rabbit liver tumor models were produced using modified culture method via laparotomic route. Healthy rabbits were randomly divided into control group and experimental group. Punctuation hepatic arteriography was performed with fine needle under the euthyphoria, control group was applied hepatic arteriography only, experimental group was embolized with calcium alginate gel. Before the embolization and 7 days after the embolization, rabbit livers were performed with CT contrast enhanced scan, to measure the volume of tumors by multiplanar reconstruction. 7 days after embolization treatment, the liver tumors and the liver tissue of tumor areas were removed and undertook histopathologic examination, to evaluate the effect of calcium alginate gel embolization on tumors and normal liver tissue.
RESULTS AND CONCLUSION: The blood of tumor was cut off after the injection of calcium alginate gel. The tumor growth rate of rabbit liver was significantly lower in the experimental group than control group (P < 0.05). In the control group, the tumors were predominantly mild necrosis; in the experimental group, the tumor was mainly severe necrosis with multiple focal necrosis. There were significant statistically differences between two groups (P < 0.01). After toluidine blue stain, we can see arteria hepatica and inter-lobe arteria, the arteriole of entering liver were obstructed completely, and the sinus hepaticus were filled with calcium alginate gel. Calcium alginate gel can form extensive embolism from liver tumor blood vascular portal to the sinus hepaticus, thoroughly stopping the blood and inhibiting the tumor growth.

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