中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (34): 6271-6275.doi: 10.3969/j.issn.1673-8225.2010.34.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

纳米珍珠层人工骨构建骨诱导型组织工程化骨治疗犬股骨头坏死

肖  颖1,2，陈建庭2，王锐英1，汤勇智2，徐俊昌2   

  1. 1桂林医学院附属医院骨外科，广西壮族自治区桂林市  541001; 2南方医科大学南方医院脊柱骨病外科，广东省广州市  510515
  • 出版日期:2010-08-20 发布日期:2010-08-20
  • 通讯作者: 陈建庭，博士生导师，南方医科大学南方医院脊柱骨病外科，广东省广州市 510515 chenjt99@yahoo.com
  • 作者简介:肖颖★，男，1977年生，湖南省邵东县人，汉族，2008年南方医科大学毕业，硕士，主治医师，主要从事脊柱骨病，骨与关节损伤方面的研究。 39668731@qq.com
  • 基金资助:

    教育部春晖计划课题基金(22005-2-45001)；广东省科技计划项目(2007B031003005)。

Treatment of femoral head necrosis in canine using inductive tissue engineered bone constructed by nano-nacre artificial bone

Xiao Ying 1,2, Chen Jian-ting2, Wang Rui-ying1, Tang Yong-zhi2, Xu Jun-chang2   

  1. 1 Department of Orthopaics Surgery, Affiliated Hospital of Guilin Medical University, Guangxi   541001, Guangxi Zhuang Autonomous Region, China; 2 Department of Orthopaedics and Spine Surgery, Nanfang Hospital of Southern Medical University, Guangzhou   510515, Guangdong Province, China
  • Online:2010-08-20 Published:2010-08-20
  • Contact: Chen Jian-ting, Doctoral supervisor, Department of Orthopaedics Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China chenjt99@yahoo.com
  • About author:Xiao Ying★, Master, Attending physician, Department of Orthopaedics Surgery, Affiliated Hospital of Guilin Medical University, Guangxi 541001, Guangxi Zhuang Autonomous Region, China; Department of Orthopaedics Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China 39668731@qq.com
  • Supported by:

    Chunhui Plan Fund by the Ministry of Education, No. 22005-2-45001*; Science and Technology Development of Guangdong Province, No. 2007B031003005*

PDF

352

被引次数

2

摘要：

背景：将生长因子和少量自体骨髓与支架材料复合构成诱导型组织工程化骨，省略了分离及体外培养细胞的步骤和费用，防止了相关的污染和细胞性状改变等，是一种现阶段较为可行的诱导骨组织再生的方法。
目的：探讨纳米珍珠层人工骨作为骨形态发生蛋白和自体骨髓载体的可行性，以及其构建的诱导型组织工程化骨治疗股骨头坏死的疗效。
方法：健康成年杂种犬建立股骨头坏死模型，分别给予髓芯减压结合植入纳米珍珠层人工骨治疗，单纯髓芯减压治疗。术后4、12周分批处死动物行影像学、生物力学及组织学检查。
结果与结论：纳米珍珠层人工骨组的抗压强度高于髓芯减压组和坏死组(P=0.000)，纳米珍珠层人工骨组新骨形成明显，表现为软骨内化骨；髓芯减压组未见新骨形成，仅纤维组织填充减压区。结果提示纳米珍珠层人工骨可以作为骨形态发生蛋白和自体骨髓的载体，其构建的诱导型组织工程化骨可改善股骨头抗压强度，促进新骨形成和进行坏死修复。

关键词: 股骨头缺血性坏死, 纳米珍珠层人工骨, 非细胞型组织工程化骨, 移植, 犬

Abstract:

BACKGROUND: The growth factor compounds a small amount of autologous bone marrow and the scaffold materials to construct the inductive tissue engineered bone, thus omitting steps and costs for isolation and in vitro culture of cells, preventing the related pollution and changes of cell trait, it is a feasible method of inducing bone regeneration.
OBJECTIVE: To investigate the feasibility of using nano-nacre artificial bone as a carrier of bone morphogenetic protein and autologous bone marrow, to the curative effect of the constructed inductive tissue engineered bone as a transplant to treat avascular necrosis of femoral head.
METHODS: Fifteen healthy hybrid dogs were used to create avascular necrosis of femoral head models. The animal models were treated by core decompression and nano-nacre artificial bone, respectively. The dogs were sacrificed respectively at 4 and 12 weeks postoperation, for radiography, biomechanics and histology examinations.
RESULTS AND CONCLUSION: The compressive strength in nano-nacre artificial bone group were significantly superior to those in core decompression group and necrosis group (P=0.000). New bone formation in nano-nacre artificial bone group was obviously significant and acted as os endochondrale. In core decompression group, the depressor areas were fibrous connective tissue, new bone formation was hardly found. Results demonstrated that it’s feasible using nano-nacre artificial bone as a carrier of bone morphogenetic protein and autologous bone marrow, the constructed constructed inductive tissue engineered bone can improve compressive strength of femoral head and promote new bone formation and repair necrosis.

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