中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (33): 6267-6270.doi: 10.3969/j.issn.1673-8225.2010.33.045

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    

南昌地区汉族绝经前女性雌激素受体α基因PvuII遗传多态性与体质量指数的关系

徐  宏1，彭  婧1，张  莉1，邹  琳2，况海斌1   

  1. 1南昌大学医学院生理学教研室，江西省南昌市  330006；2江西省人民医院放射免疫室，江西省南昌市  330006
  • 出版日期:2010-08-13 发布日期:2010-08-13
  • 通讯作者: 况海斌，男，博士，副教授，南昌大学医学院生理学教研室，江西省南昌市330006
  • 作者简介:徐 宏★，女，1978年生，江西省南昌市人，汉族，2005年湖南师范大学毕业，硕士，讲师，主要从事内分泌生理研究。
  • 基金资助:

    国家自然科学基金(编号30760071、30960118)，江西省教育厅基金(编号GJJ08106)和南昌大学科研课题基金(编号J06)。

Correlating estrogen receptor-alpha PvuII genetic polymorphism with bone mass index in premenopausal Han Chinese women from Nanchang region

Xu Hong1, Peng Jing1, Zhang Li1, Zou Lin2, Kuang Hai-bin1   

  1. 1 Department of Physiology, School of Medicine, Nanchang University, Nanchang   330006, Jiangxi Province, China;2 Department of Radioimmunology, People's Hospital of Jiangxi Province, Nanchang  330006, Jiangxi Province, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Kuang Hai-bin, Doctor, Associate professor, Department of Physiology, School of Medicine, Nanchang University, Nanchang 330006, Jiangxi Province, China khb9909@yahoo.com.cn
  • About author:Xu Hong★, Master, Lecturer, Department of Physiology, School of Medicine, Nanchang University, Nanchang 330006, Jiangxi Province, China hongxu1978@ yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30760071*, 30960118*; A Grant from Education Department of Jiangxi Province, No. GJJ08106*; Scientific Research Foundation of Nanchang University, No.J06*

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摘要：

背景：肥胖是由多基因决定的复杂性疾病，体质量指数是研究肥胖的常用表型。有研究显示一些候选基因与体质量指数变异相关，然而它们之间的相互关系却仍未阐明。
目的：探讨雌激素受体α基因(ER-α)PvuII多态性是否与体质量指数的变异相关联。
方法：征集了绝经前女性390例，均来自南昌地区江西省人民医院的体检人员，测量其身高和体质量，计算体质量指数。用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法对所有被试者的雌激素受体α基因PvuII位点进行基因分型。
结果与结论：雌激素受体α基因PP，Pp和pp基因型频率分别为0.133，0.485和0.382。雌激素受体α基因型分布符合哈迪-温伯格平衡(P > 0.05)。雌激素受体α基因PvuII与体质量指数没有显著的关联性(P= 0.338)。证实雌激素受体α基因PvuII多态性对南昌地区绝经前妇女体质量指数没有重要的影响。

关键词: 绝经前女性, 体质量指数, 雌激素受体基因, 遗传关联, 遗传多态性

Abstract:

BACKGROUND: Obesity is serious complex disease determined by multiple genetic factors. Body mass index (BMI) is a common phenotype for obesity. Although some candidate genes have been shown to be related with BMI variation, the underlying substantial relationship remains poorly understood.
OBJECTIVE: To investigate whether estrogen receptor-α (ER-α) PvuII genetic polymorphism is associated with BMI variation.
METHODS: In total 390 premenopausal women from a local population of Nanchang City in Jiangxi Province People's Hospital. Body weight and height were measured. All participants were genotyped at the ER-α PvuII locus using polymerase chain reaction-restriction fragment length polymorphism.
RESULTS AND CONCLUSION: The ER-α PvuII genotype frequencies of PP, Pp and pp were 0.133, 0.485 and 0.382, respectively. The distribution of ER-PvuII genotypes was in accordance with Hardy-Weinberg equilibrium (HWE) (P > 0.05). There was no significant correlation between ER-α PvuII genetic polymorphism and BMI variation (P = 0.338). These findings indicate that ER-α PvuII genetic polymorphism does not greatly affect BMI variation of premenopausal women in Nanchang region.

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