中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (32): 6015-6019.doi: 10.3969/j.issn.1673-8225.2010.32.027

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

Sca-1+造血干/祖细胞重建致死剂量辐射小鼠造血功能的作用

周   玥，杨   斌，姚   欣，王亚平   

  1. 重庆医科大学干细胞与组织工程研究室，组织学与胚胎学教研室，重庆市  400016
  • 出版日期:2010-08-06 发布日期:2010-08-06
  • 通讯作者: 王亚平，教授，博士生导师，重庆医科大学组织学与胚胎学教研室，干细胞与组织工程研究室，重庆市 400016 ypwangcq@ yahoo.cn
  • 作者简介:周玥☆，女，1980年生，云南省大理市人，白族，重庆医科大学组织学与胚胎学教研室在读博士，讲师，主要从事造血干细胞衰老生物学的研究。 zhouyue_120@ sina.com
  • 基金资助:

    国家自然科学基金资助项目(30973818)；重庆市科委自然科学基金重点项目(CSTC，2009BA5038)。

Hematopoietic reconstruction in mice treated with lethal dose irradiation using Sca-1+ hematopoietic stem/progenitor cells transplantation 

Zhou Yue, Yang Bin, Yao Xin, Wang Ya-ping   

  1. Research Room of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing  400016, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Wang Ya-ping, Professor, Doctoral supervisor, Research Room of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China ypwangcq@yahoo.cn
  • About author:Zhou Yue☆, Studying for doctorate, Lecturer, Research Room of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China zhouyue_120@sina. com
  • Supported by:

    the National Natural Science Foundation of China, No. 30973818*; the Natural Science Foundation of Scientific Committee of Chongqing City, No.CSTC,2009BA5038*

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摘要：

背景：近年来，造血干细胞移植在国内外已广泛开展，移植的造血干细胞能否有效重建造血成为造血干细胞移植领域的研究热点问题之一。
目的：观察Sca-1+造血干/祖细胞(hematopoietic stem/progenitor cell，Sca-1+HSC/HPC)对造血衰竭小鼠造血功能重建的作用。
方法：雌性C57BL/6受体鼠给予致死剂量60Coγ射线照射后分2组，对照组：输注无菌PBS；移植组：移植免疫磁性分选法分离纯化的同系雄性C57BL/6小鼠Sca-1+HSC/HPC；检测受体鼠生存时间；外周血白细胞、红细胞比容、血小板的变化；脾湿质量及脾集落数；PCR检测Y染色体(Sry基因)表达确定受体小鼠重建造血的细胞来源。
结果与结论：移植组受体鼠30 d存活率、白细胞、红细胞比容、血小板、脾湿质量及脾集落数均明显高于对照组(P < 0.05)；Y染色体PCR分析，证实雌性受体小鼠重建造血的细胞来源于雄性供体。提示Sca-1+HSC/HPC移植后能重建造血衰竭小鼠造血功能。

关键词: 造血功能, Sca-1+造血干/祖细胞, 分离纯化, 重建造血, 小鼠

Abstract:

BACKGROUND: In recent years, hematopoietic stem cell transplantation has widely used in clinic. The effectiveness of HSC reconstruction of the hematopoiesis has became a hot topic in the field of HSCT.
OBJECTIVE: To study hematopoietic reconstruction for hematopoietic failure mice with the transplantation of Sca-1+ hematopoietic stem/progenitor cells (Sca-1+HSC/HPC).
METHODS: The female C57BL/6 recipient mice received a lethal dose irradiation from 60Coγ ray and were divided into two groups. The control group mice were injected with sterile phosphate buffered saline. The transplantation group mice were transplanted with Sca-1+HSC/HPC isolated by magnetic cell sorting from homologous series male mice. After transplantation, the survival time, the change of peripheral blood leucocyte, haematocrit and platelet, the weight of spleen, the number of colony forming unit-spleen (CFU-S) of recipient mice were observed. Polymerase chain reaction (PCR) was used to measure Y chromosome (Sry gene) to verify the cell source of hematopoietic reconstruction in receipient mice.
RESULTS AND CONCLUSION: The 30-day survival rate, the number of leucocyte, haematocrit and platelet, the weight of spleen and the number of CFU-S were significantly higher in transplantation group compared with control group (P < 0.05). Y chromosome PCR results indicated that reconstructed hematopoietic cells of female recipient mice were derived from male donor. These suggested that Sca-1+HSC/HPC transplantation can reconstruct hematopoietic function of hematopoietic failure mice.

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