中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (32): 5931-5934.doi: 10.3969/j.issn.1673-8225.2010.32.009

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

亚低温与脑缺血大鼠骨髓间充质干细胞的迁移

游  辉1，彭  玉1，张其梅1，李承晏2   

  1. 1三峡大学第一临床医学院，湖北省宜昌市  443003；2武汉大学人民医院神经科，湖北省武汉市430070
  • 出版日期:2010-08-06 发布日期:2010-08-06
  • 通讯作者: 彭玉，硕士，主治医师，三峡大学第一临床医学院，湖北省宜昌市443003 ycpengu@126. com
  • 作者简介:游辉，湖北省麻城市人，汉族，主治医师，主要从事干细胞治疗方面的研究。

Mild hypothermia effects on migration of bone marrow mesenchymal stem cells in rats with cerebral ischemia 

You Hui1,Peng Yu1,Zhang Qi-mei1,Li Cheng-yan2   

  1. 1 First Clinical Medical College, Three Gorges University, Yichang  443003, Hubei Province, China; 2 Department of Neurology, Renmin Hospital, Wuhan University, Wuhan  430070, Hubei Province, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Peng Yu, Master, Attending physician, First Clinical Medical College, Three Gorges University, Yichang 443003, Hubei Province, China ycpengu@126.com
  • About author:You Hui, Attending physician, First Clinical Medical College, Three Gorges University, Yichang 443003, Hubei Province, China

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305

被引次数

3

摘要：

背景：关于亚低温运用到神经损伤修复领域的研究已有一些报道，但亚低温对神经干细胞在脑内移植迁移的影响还不太清楚。
目的：观察亚低温对移植入脑缺血大鼠侧脑室的骨髓间质干细胞迁移的影响。
方法：采用Longa法永久性闭塞SD大鼠大脑中动脉制作局灶性脑缺血损伤模型，亚低温组于移植前应用亚低温处理大鼠急性脑缺血损伤，对照组于移植前应用常温处理大鼠急性脑缺血损伤；造模术后24 h，在脑立体定向下，经侧脑室注射移植用5-BrdU标记的骨髓间充质干细胞。经过5，14，21，28 d，7周后用免疫组织化学方法检测各组大鼠脑组织BrdU阳性细胞数。
结果与结论：移植第14天多数标记的骨髓基质细胞细胞已经迁移至梗死灶周围，移植后各时间点亚低温组梗死灶周边皮质的BrdU阳性细胞数明显多于对照组(P < 0.05)。提示移植前宿主亚低温处理可以促进骨髓间充质细胞的定向迁移，对局灶性脑缺血有神经保护作用。

关键词: 骨髓间充质干细胞, 亚低温, 迁移, 脑缺血, 移植

Abstract:

BACKGROUND: There are a few reports addressing application of mild hypothermia to the study of repairing nerve injury, but few reports have addressed mild hypothermia effects on migration of neural stem cells in the brain following transplantation.
OBJECTIVE: To explore the effects of mild hypothermia on migration of BMSCs transplanted into lateral ventricle of rats after cerebral ischemia.
METHODS: A focal cerebral ischemic injury model of right middle cerebral artery occlusion (MCAO) was established using modified Longa’s method in Sprague Dawley rats. Local mild hypothermia was applied in the mild hypothermia group prior to transplantation. Normal body temperature was maintained in control group before transplantation. At 24 hours following model establishment, BMSCs labeled with 5-BrdU were transplanted into the rat lateral ventricle under brain stereotaxis. The amount of BrdU-positive cells was measured by immunohistochemistry at 5, 14, 21, 28 days and 7 weeks following injection in each group.
RESULTS AND CONCLUSION: At 14 days after transplantation, a majority of labeled BMSCs had migrated to the infarct area. At various time points following transplantation, the number of BrdU-positive cells was obviously greater in the hypothermia group compared with the control group (P < 0.05). Results have indicated that mild hypothermia may promote the migration of BMSCs, and shows neuroprotective effects on focal cerebral ischemia.

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