中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (25): 4614-4618.doi: 10.3969/j.issn.1673-8225.2010.25.014

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

Fe3O4空心磁纳米微球制备工艺条件的影响因素

彭  健，唐  琦，潘一峰，唐静波，李  波，陈  伟，崔  健，张阳德   

  1. 中南大学湘雅医院卫生部纳米生物技术重点实验室，湖南省长沙市 410008
  • 出版日期:2010-06-18 发布日期:2010-06-18
  • 通讯作者: 张阳德，博士生导师，教授，主任，中南大学湘雅医院卫生部纳米生物技术重点实验室，湖南省长沙市 410008 nhec@2118.cn
  • 作者简介:彭 健☆，男，1969年生，湖南省衡阳市人，汉族，2000年中南大学湘雅医院外科学毕业，博士，副教授，主要从事磁纳米载体的靶向治疗研究。 kittom@163.com
  • 基金资助:

    国家“863”项目(2007AA021806)，项目名称：磁性空心球纳米药物制剂靶向治疗恶性肿瘤。国家“863”项目(2007AA021804)，项目名称：多功能金属壳热化疗纳米载药系统靶向治疗恶性肿瘤。

Influential factors of preparing hollow superparamagnetic Fe3O4 nanospheres

Peng Jian, Tang Qi, Pan Yi-feng, Tang Jing-bo, Li Bo, Chen Wei, Cui Jian, Zhang Yang-de   

  1. National Key Laboratory of Nanobiological Technology, Xiangya Hospital, Central South University, Changsha   410008, Hunan Province, China
  • Online:2010-06-18 Published:2010-06-18
  • Contact: Zhang Yang-de, Doctoral supervisor, Professor, National Key Laboratory of Nanobiological Technology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China nhec@2118.cn
  • About author:Peng Jian☆, Doctor, Associate professor, National Key Laboratory of Nanobiological Technology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China kittom@163.com
  • Supported by:

    National 863 Program, No. 2007AA021806*;  2007AA021804*

PDF

551

摘要：

背景：空心微球材料具有很好的生物相容性，在生物医学和化学等许多领域得到广泛的应用。
目的：分析Fe3O4空心磁性纳米微球制备流程中反应温度、反应时间、洗涤次数、超声时间工艺条件对粒径的影响。
方法：以聚氧乙烯-聚氧丙烯-聚氧乙烯-嵌段共聚物F127为原料，用共沉淀法制备空心超顺磁性纳米微球。运用正交试验的数学方法设计一个4×3的正交实验模型，从反应温度、反应时间、洗涤次数、超声时间4个方面考察不同制备条件对空心磁性纳米微球粒径大小的影响。
结果与结论：得到的最佳实验制备条件：在75 ℃下，充分反应6 h，用甲醇/去离子水反复洗涤3次之后，用功率为30%的超声处理20 min。样品通过0.22 µm滤嘴过滤后，用粒径分布仪、X射线衍射和透射电子显微镜对产物进行表征观察，证实用最优的实验条件制备的空心Fe3O4磁性纳米微球粒径为37.5 nm，外壳厚度10 nm左右，颗粒大小均匀，分布较好。

关键词: 工艺条件, Fe3O4, 正交实验, 空心纳米微球, Pluronic F-127

Abstract:

BACKGROUND: Hollow microspheres exhibit good biocompatibility and have been widely used in many fields such as biomedicine and chemistry.
OBJECTIVE: To study the affecting factors including the reaction temperature, reaction time, the number of washing, ultrasonic time in preparation process conditions on the particle size of hollow superparamagnetic Fe3O4 nanospheres.
METHODS: With polyoxyethylene-polyoxypropylene-polyoxyethylene-block copolymer F127 as raw materials, Fe3O4 hollow superparamagnetic nanospheres were prepared by coprecipitation methods. Using mathematical methods of orthogonal experiment, a 4 × 3 orthogonal experimental model was designed. The influence of different preparation conditions such as the reaction temperature, reaction time, the number of washing and ultrasonic time, on the size of hollow magnetic nanoparticles was determined.
RESULTS AND CONCLUSION: The optimal preparation conditions were: 75 ℃, fully reacting 6 hours, washing three times using methanol/deionized water, 30% power ultrasonic treatment for 20 minutes. After filtration by a 0.22-µm filter, the samples were characterized by particle size analyzer, X-ray diffraction and transmission electron microscopy. The average diameter of Fe3O4 hollow magnetic nanospheres was 37.5 nm, shell thickness was about 10 nm, particle size and distribution were even.

中图分类号: