中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2709-2713.doi: 10.3969/j.issn.1673-8225.2010.15.012

• 血管组织构建 vascular tissue construction • 上一篇    下一篇

雷帕霉素滴眼剂抑制大鼠角膜血管新生：血管内皮生长因子及炎症因子的作用

钟彦彦1，张海峰2，陆晓和1   

  1. 1南方医科大学珠江医院眼科，广东省广州市 510282；2华南理工大学，广东省广州市  510641
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 张海峰，主治医师，华南理工大学，广东省广州市 510641 surgeonzhf@163.com
  • 作者简介:钟彦彦☆，女，1980年生，广东省蕉岭县人，汉族，南方医科大学在读博士，主要从事角膜病研究。 zhongyanyan07@163.com

Rapamycin eye drops inhibit rat corneal neovascularization: Role of vascular endothelial growth factor and inflammatory factor

Zhong Yan-yan1, Zhang Hai-feng2, Lu Xiao-he1   

  1. 1 Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou   510282, Guangdong Province, China; 2 South China University of Technology, Guangzhou   510641, Guangdong Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Zhang Hai-feng, Attending physician, South China University of Technology, Guangzhou 510641, Guangdong Province, China surgeonzhf@163.com
  • About author:Zhong Yan-yan☆, Studying for doctorate, Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China zhongyanyan07@163.com

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摘要：

背景：雷帕霉素全身用药可明确抑制角膜移植后植片新生血管的生长，但雷帕霉素滴眼剂能否起到相同或者类似的作用尚有待验证。
目的：观察雷帕霉素滴眼剂抑制大鼠角膜新生血管生长过程中血管内皮生长因子及炎症因子的作用。
方法：角膜缝线法建立SD大鼠左眼角膜新生血管模型，以抽签法随机分为生理盐水对照组与雷帕霉素滴眼剂组。各组均术后第1天开始用药，4次/d，1滴/次。每日行裂隙灯显微镜检查，术后第3，7，14天测量角膜新生血管的长度，计算新生血管面积。14 d后切取角膜行组织学检查，并采用免疫组织化学染色法检测血管内皮生长因子以及白细胞介素2受体α的表达。
结果与结论：生理盐水对照组不同时间点角膜新生血管面积均高于雷帕霉素滴眼剂组(P=0)，说明雷帕霉素滴眼剂可抑制缝线后角膜新生血管的生长。与生理盐水对照组比较，雷帕霉素滴眼剂组角膜上皮层、浅基质层、新生血管内皮、角膜缝线处上皮以及浸润的炎性细胞内血管内皮生长因子及白细胞介素2受体α表达明显降低，病理切片上炎性细胞浸润减少。结果提示雷帕霉素滴眼剂抑制角膜新生血管生长，可能通过降低血管内皮生长因子的表达及抑制炎性细胞的分化浸润从而抑制新生血管的生长。

关键词: 西罗莫司, 角膜, 新生血管, 血管内皮生长因子, 白细胞介素2受体&alpha, 眼科组织工程

Abstract:

BACKGROUND: Systemic administration of rapamycin has been demonstrated to inhibit graft neovascularization following corneal transplantation. However, the effect of rapamycin eye drops remains unknown.
OBJECTIVE: To explore the role of vascular endothelial growth factor (VEGF) and inflammatory factor during the inhibition of rapamycin eye drops on corneal neovascularization.
METHODS: Corneal neovascularization in the left eye was induced by suture method, and the 12 SD rats were randomly assigned to normal saline and rapamycin groups. Drugs were delivered, 1 drop each, 4 times daily since 1 day after operation. Rat corneas were checked by slit lamp microscope daily. The length of corneal neovascularization was measured at days 3, 7, and 14 to calculate the area of corneal neovascularization. The expression of VEGF and interleukin-2 receptor α (IL-2Rα) was detected by immunohistochemical techniques at 14 days.
RESULTS AND CONCLUSION: Corneal neovascularization area of normal saline group was significantly greater than rapamycin group at each time point (P=0), indicating that rapamycin eye drop inhibited corneal neovascularization following suture. Compared with normal saline group, the expression of VEGF and IL-2Rα was significantly decreased in corneal epithelia, stroma, corneal neovascular endothelium and epithelium at the suture site, as well as inflammatory cells of rapamycin group. Moreover, the inflammatory cell infiltrate was reduced in the pathological sections of rapamycin group. Results show that rapamycin eye drops inhibit corneal neovascularization, possibly by reducing the expression of VEGF and IL-2Rα.

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