中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (14): 2612-2616.doi: 10.3969/j.issn.1673-8225.2010.14.029

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

Hes1在成年小鼠脑中表达的免疫组织化学分析

郇林春1，杨新宇1，赵旺淼1，赵  岩1，2，赵  杰1，3，张  奇1，杨树源1   

  1. 1天津医科大学总医院神经外科，天津市神经病学研究所，天津市神经损伤变异与再生重点实验室，天津市  300052；2解放军总医院神经外科，北京市  100853；3天津市人民医院神经内科，天津市  300121
  • 出版日期:2010-04-02 发布日期:2010-04-02
  • 通讯作者: 杨新宇，博士，教授，主任医师，硕士生导师，天津医科大学总医院神经外科，天津市神经病学研究所，天津市神经损伤变异与再生重点实验室，天津市 300052 paperxyyang@gmail.com
  • 作者简介:郇林春★，男，1982年生，山东省临沂市人，汉族，天津医科大学在读硕士，主要从事脑创伤和脑血管病的外科治疗研究。 uqqc@163.com
  • 基金资助:

    国家自然科学基金资助项目(30973090)：Hes1蛋白在修复小鼠创伤性海马损伤中所起作用的研究。天津市自然科学基金资助项目(09JCZDJC17200)：Hes1蛋白促进成年神经细胞生成修复脑损伤的研究。天津市高等学校科技发展基金计划项目(20070202)：脑创伤后HES1促进神经细胞再生修复脑损伤的研究。天津市卫生局科技基金资助项目：急性脑创伤后脑组织内神经细胞新生现象的研究。

Immunohistochemical analysis of the expression of Hes1 in adult mouse brain

Huan Lin-chun1, Yang Xin-yu1, Zhao Wang-miao1, Zhao Yan 1, 2, Zhao Jie 1, 3, Zhang Qi1, Yang Shu-yuan1   

  1. 1 Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin Neurology Institute, Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin  300052, China; 2 Department of Neurosurgery, Chinese People’s Liberation Army General Hospital, Beijing  100853, China; 3 Department of Neurology, Tianjin People’s Hospital, Tianjin  300121, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Yang Xin-yu, Doctor, Professor, Chief physician, Master’s supervisor, Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin Neurology Institute, Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin 300052, China paperxyyang@gmail.com
  • About author:Huan Ling-chun★, Studying for master’s degree, Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin Neurology Institute, Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin 300052, China uqqc@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30973090*; Natural Science Foundation of Tianjin, No. 09JCZDJC17200*; Scientific Research Development Program of the Higher Education Institutions of Tianjin, No. 20070202*; Foundation of Health Bureau of Tianjin*

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669

被引次数

21

摘要：

背景：近年来的研究显示，在胚胎神经系统发育过程中，Hes1的表达调控对保持神经干细胞的数量、调控其分化至关重要。此外，成年个体内处于静止期的纤维母细胞重新获得分裂增殖的能力需要Hes1表达的上调。这表明Hes1在成体中也与某些潜在干细胞的增殖具有密切的关系。因此研究Hes1在成体神经干细胞中的表达及其与成体神经细胞生成的关系也就被提上日程。但Hes1在成年个体神经系统的表达至今未明。
目的：观察和分析小鼠脑中不同部位Hes1的表达及Hes1阳性细胞的细胞类型。
方法：12只成年雄性C57BL/6小鼠随机数字表法分为单染组和双染组，每组6只。单染组直接取材，采用免疫组织化学技术检测Hes1在小鼠脑中各部位的表达。双染组小鼠以200 mg/kg Brdu的剂量每天腹腔注射1次，连续注射3 d，第4天取材，采用双标记物染色观察分析海马区Hes1阳性细胞的细胞类型。
结果与结论：在所有观察的解剖部位中，Hes1表达于所有存在神经细胞的部位，Brdu阳性细胞几乎全部表达Hes1，NeuN阳性细胞全部表达Hes1，而神经胶质原纤维酸性蛋白阳性细胞则完全不表达Hes1。由此可知，Hes1表达于神经细胞和神经干细胞中，胶质细胞不表达Hes1。

关键词: 海马, Hes1, 神经细胞生成, 神经干细胞, 免疫组织化学, 小鼠

Abstract:

BACKGROUND: In recent years, it has been shown in studies that regulation of the expression of Hes1 is very important to maintain neural stem cells and regulate its differentiation during the development of embryonic nervous system. Moreover, upregulation of Hes1 expression is required for quiescent adult fibroblasts resuming proliferation. This indicates that there is a close relationship between Hes1 and some potential stem cells in adult. Therefore, the study of Hes1 expression in neural stem cells and its relationship with adult neurogenesis is put on the agenda. However, the expression of Hes1 in adult brain is still not clear.
OBJECTIVE: To observe the expression of Hes1 in adult mouse brain and the cell types of Hes1 positive cells.
METHODS: Totally 12 adult male C57BL/6 mice were randomly evenly divided into two groups by using the random number table. For the first group, the mouse brain was removed directly and immunohistochemical staining was used to detect the expression of Hes1 in the adult mouse brain. For the second group, Brdu was injected intraperitoneally to every mouse with a dosage of 200 mg/kg, once a day, for 3 days. The mouse brain was removed at 4 days and then double staining was carried out to observe and analysis the cell types of Hes1 positive cells in hippocampus.
RESULTS AND CONCLUSION: Hes1 was expressed in all the observed anatomical site where neural cells exist, Brdu positive cells nearly all expressed Hes1, NeuN positive cells all expressed Hes1, whereas GFAP positive cells did not express Hes1 completely. Hes1 was expressed in all neurons and neural stem cells, and glial cells did not express Hes1.

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