中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (13): 2324-2428.doi: 10.3969/j.issn.1673-8225.2010.13.012

• 骨科植入物 orthopedic implant • 上一篇    下一篇

个性化钛模板作为外支架与组织工程化骨联合修复兔上槽嵴缺损：维持新骨塑形的可行性

葛一鸣，亓  峰，王稚英   

  1. 辽宁医学院附属第二医院，辽宁省锦州市  121001
  • 出版日期:2010-03-26 发布日期:2010-03-26
  • 通讯作者: 王稚英，教授，博士，辽宁医学院附属第二医院，辽宁省锦州市 121001
  • 作者简介:葛一鸣★，女，1983年生，辽宁省朝阳市人，辽宁医学院在读硕士，医师，主要从事颌骨修复与重建研究。 165166347@qq.com
  • 基金资助:

    辽宁省自然科学基金(20092186)，项目名称：个性化钛模板作为组织工程外支架修复牙种植体周围骨量不足的实验研究。辽宁省教育厅高等学校科学研究项目(05L138)，项目名称：个性化钛网、钛板联合重建中空上颌骨的临床和基础研究。

Personalized prefabricated titanium template and tissue-engineered bone in reconstruction of maxillary alveolar defect in rabbits: Feasibility to maintain new bone shape

Ge Yi-ming, Qi Feng, Wang Zhi-ying   

  1. Second Affiliated Hospital of Liaoning Medical University, Jinzhou   121001, Liaoning Province, China
  • Online:2010-03-26 Published:2010-03-26
  • Contact: Wang Zhi-ying, Professor, Doctor, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • About author:Ge Yi-ming★, Studying for master’s degree, Physician, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China 165166347@qq.com
  • Supported by:

    the Natural Science Foundation of Liaoning Province, No. 20092186*; the Scientific Research Program of Liaoning Provincial High Institutes, No. 05L138*

PDF

371

摘要：

背景：在口腔组织工程学中，由于颌面骨形态复杂，不规则，而且颌骨支撑着颜面部，与容貌有很大的关联。这就提出了更高要求，不但要恢复功能而且要恢复原有形态，而且精密的修复形态将成为今后发展的趋势。
目的：探讨透明质酸钠与复合重组人骨形态发生蛋白2诱导兔骨髓基质干细胞体外构建组织工程化骨修复上颌骨牙槽嵴缺损，并覆盖个性化钛模板作为外支架维持新骨塑形的可能性。
方法：体外培兔骨髓基质干细胞，进行成骨诱导，复合透明质酸钠、重组人骨形态发生蛋白2构建组织工程化骨，植入自体牙槽嵴缺损处，外加个性化钛模板，术后4，8，12 周处死动物分别进行X射线、常规组织学检查，观测组织工程化骨在体内成骨作用。以缺损区植入回收自体骨屑者为对照组。
结果与结论：术后第4周，实验组与对照组新生骨灰度值差异有显著性意义(P < 0.05)。术后第8，12周其新骨灰度值差异无显著性意义(P > 0.05)，术后4周与术后8周，术后8周与术后12周新生骨灰度值存在显著性差异(P < 0.05)。常规组织学染色显示，在术后4周时，实验组成骨作用逊色于对照组。当生长到第8周时，两组成骨基本无显著差异。说明此法构建的组织工程化骨在体内成骨效应显著，个性化钛模板起到屏障塑形作用，促进新骨按特定形状生成。

关键词: 组织工程化骨, 颌骨缺损, 重组人骨形态发生蛋白2, 骨髓间充质干细胞, 个性化钛模板

Abstract:

BACKGROUND: In oral tissue engineering, appearance of maxillofacial bone is complex and irregular, and maxillofacial bone supports the face, and highly correlates to this appearance. Therefore, in addition to restoring the function, appearance restoration is also important. Precise appearance restoration would become developing trends.
OBJECTIVE: To explore the effect of tissue-engineered bone constructed by hyaluronic acid in combination with recombinant bone morphogenetic protein (rhBMP-2)-induced bone marrow stromal stem cells (MSCs) in vitro on repairing maxillary alveolar ridge defect, and investigate the feasibility to maintain moulding by personalized template for the external fixation of titanium.
METHODS: Rabbit MSCs were cultured in vitro for bone induction to construct tissue-engineered bone in combination with sodium hyaluronate and rhBMP-2. The bone was implanted into the autologous alveolar ridge defect, plus personalized titanium template. After 4, 8, 12 weeks, animals were sacrificed and respectively subjected to radiation, conventional histological examination to observe in vivo osteogenesis of this tissue-engineered bone. In addition, rats implanted with autogenous bone fragments served as control.
RESULTS AND CONCLUSION: There were significant differences in gray scale between experimental and control groups at the first 4 weeks (P < 0.05). But at 8 and 12 weeks, no differences were observed (P > 0.05). In addition, there were significant differences in gray scale of newly-generated bone between 4 and 8 weeks and 8 and 12 weeks after operation (P < 0.05). Histological staining showed that osteogenesis of the experimental group was less than the control group at 4 weeks, and became similar at 8 weeks. Results show that the tissue-engineered bone constructed with hyaluronic acid sodium and rhBMP2F-induced MSCs displayed obvious osteogenesis, and personalized shaping titanium barriers play a role in the template to promote the new bone generated by a particular shape.

中图分类号: