中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (8): 1372-1376.doi: 10.3969/j.issn.1673-8225.2010.08.011

• 药物控释材料 drug delivery materials • 上一篇    下一篇

微囊化兔许旺细胞移植脊髓损伤大鼠：碱性成纤维细胞生长因子表达和后肢运动功能的变化

陈  惠1，傅文学2，桂  婷1，刘德明1，杨耀防2   

  1. 1南昌大学医学院人体解剖教研室，江西省南昌市 330006；2九江学院医学院人体解剖教研室，江西省九江市 332000
  • 出版日期:2010-02-19 发布日期:2010-02-19
  • 通讯作者: 杨耀防，硕士，教授，九江学院医学院人体解剖教研室，江西省九江市 332000 yfy457@sohu.com
  • 作者简介:陈 惠，女，1971年生，江西省九江市人，汉族，2001年南昌大学毕业，主治医师，主要从事脊髓损伤与修复方面的研究。 chen8200233@qq.com
  • 基金资助:

    江西省卫生厅科技项目（20072049），课题名称“微囊化兔坐骨神经细胞对大鼠损伤脊髓碱性成纤维细胞生长因子表达的研究”。

Transplantation of microencapsulated rabbit schwann cells in rats after spinal cord injury: Basic fibroblast growth factor expression and hindlimb movement function changes

Chen Hui1, Fu Wen-xue2, Gui Ting1, Liu De-ming1, Yang Yao-fang2   

  1. 1 Staff Room of Human Anatomy, Medical College of Nanchang University, Nanchang   330006, Jiangxi Province, China; 2 Staff Room of Human Anatomy, Medical College of Jiujiang University, Jiujiang   332000, Jiangxi Province, China
  • Online:2010-02-19 Published:2010-02-19
  • Contact: Yang Yao-fang, Master, Professor, Staff Room of Human Anatomy, Medical College of Jiujiang University, Jiujiang 332000, Jiangxi Province, China yfy457@sohu.com
  • About author:Chen Hui, Attending physician, Staff Room of Human Anatomy, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China chen8200233@qq.com
  • Supported by:

    the Technology Program of Health Department of Jiangxi Province, No. 20072049*

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摘要：

背景：研究表明微囊化兔许旺细胞移植于大鼠损伤脊髓后，能减轻炎症反应，促进脊髓再生，但具体作用途径尚不完全清楚。
目的：观察微囊化兔许旺细胞移植于损伤大鼠脊髓后，碱性成纤维细胞生长因子的表达以及后肢运动功能的恢复。
方法：取家兔坐骨神经以双酶消化法制成许旺细胞悬液后，再用气体喷入法制成海藻酸钡-许旺细胞微囊。同法制备不包被许旺细胞的空囊。SD大鼠随机分为4组：细胞组、空囊组、微囊组建立脊髓半横断损伤模型，分别于损伤处植入明胶海绵吸附的10 μL许旺细胞悬液、10 μL空囊、10 μL海藻酸钡-许旺细胞微囊；正常组不做任何干预。采用BBB评分观察大鼠后肢运动功能恢复情况，制作脊髓标本切片通过苏木精-伊红染色和尼氏染色行病理学检查，免疫组织化学染色观察碱性成纤维细胞生长因子的表达变化。
结果与结论：造模后即刻大鼠右后肢出现瘫痪；材料移植后7，14，28 d，微囊组大鼠后肢运动功能明显恢复，BBB评分明显优于细胞组、空囊组(P < 0.05或P < 0.01)。碱性成纤维细胞生长因子阳性细胞主要见于神经元细胞的胞浆及胶质细胞的胞核内。材料移植后第1，3，7天，胶质细胞主要在脊髓损伤附近处表达，其中第3天表达量最大；第14天在神经元细胞的胞浆内可见碱性成纤维细胞生长因子表达，微囊组表达程度明显高于细胞组、空囊组；之后各组表达程度均明显下降。提示微囊化兔许旺细胞移植于损伤大鼠脊髓后，可以抑制异种移植后免疫排斥，减轻炎症反应，增加碱性成纤维细胞生长因子表达，促进后肢功能恢复和脊髓再生。

关键词: 脊髓损伤, 移植, 碱性成纤维细胞生长因子, 运动功能, 微囊, 许旺细胞, 生物材料

Abstract:

BACKGROUND: Transplantation of microencapsulated rabbit Schwann cells in the rat spinal cord can relieve inflammatory reaction, promote spinal cord regeneration, but the precise mechanisms remain unclear.
OBJECTIVE:To observe basic fibroblast growth factor (bFGF) expression and movements recovery following transplantation of microencapsulated rabbit Schwann cells in rat spinal cord.
METHODS: The sciatic nerves taken out from rabbits were digested with mixed enzyme and were made into Schwann cells suspension. Then we used air-jet method to make Schwann cells microcapsule. Using the same method, empty microcapsule was made. Sprague Dawley rats were randomly divided into cell group, empty microcapsule group and microcapsule group. Conducted by hemisection injury of spinal cord,the rats in cell group,empty microcapsule group and microcapsule group were implanted with gelatin sponge with 10 μL Schwann cells suspension, gelatin sponge with 10 μL empty microcapsule and 10 μL microencapsulated Schwann cells. Normal group was left intact. After operation, we observed hindlimb movements recovery in rats with the Basso, Beattie, and Bresnahan (BBB) scale. Meanwhile,a set of sections were stained immunohistochemically for bFGF expression, another set of sections were stained for hematoxylin-eosin and Nissal.
RESULTS AND CONCLUSION: After spinal cord injury, rat right hindlimb affected paralysis immediately. At 7, 14 and 28 days following transplantation,motor function in rat hindlimb was significantly recovered, and the BBB scores were significantly higher in microencapsulated schwenn cells than in cell and empty microcapsule group (P < 0.05 or P < 0.01). bFGF positive products were mainly distributed in cytoplasm of the spinal neuron and nucleus of neuroglical cell. The numbers of bFGF positive glial cells mainly appeared surrounding the spinal cord injured site on days 1, 3, 7 and rose to its peak on day 3 and began to appear in neuronal cells on day 14. The number of bFGF positiv cells in microcapsule group was significantly superior to that in cell group and empty microcapsule group. From then on, the bFGF expression was significantly decreased in each group. These indicated that transplantation of microencapsulated Schwann cells can inhibit the immunological rejection after xenotransplantation, suppress inflammatory reaction, improve the expression of bFGF, increase hindlimb movements recovery and spinal cord regeneration after spinal cord injury.

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