Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5197-5200.doi: 10.3969/j.issn.1673-8225.2011.28.017

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Establishment of hydrogen peroxide-induced cataract model in vitro and apoptosis of lens epithelial cells

Liu Zhen-zhen, Li Ping-hua, Tao Shu-xin, Yang Yang   

  1. Department of Ophthalmology, First Affiliated Hospital, Chongqing Medical University, Chongqing  400016, China
  • Received:2011-02-06 Revised:2011-05-07 Online:2011-07-09 Published:2011-07-09
  • Contact: Li Ping-hua, Professor, Department of Ophthalmology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China liuzhenzhen0828@163.com
  • About author:Liu Zhen-zhen★, Studying for master’s degree, Department of Ophthalmology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China liuzhenzhen0828@163.com

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Abstract:

BACKGROUND: Oxidative stress can induce apoptosis in lens epithelial cells (LECs).
OBJECTIVE: To observe the connection of Fas with apoptosis of LECs in hydrogen peroxide (H2O2)-induced cataract and the effect of epigallocatechin-3-gallate (EGCG) on the expression of Fas and apoptosis of LECs.
METHODS: Lenses isolated from healthy adult rabbits were randomly divided into the blank control group (treated with DMEM), the H2O2 group (treated by DMEM + H2O2), and the EGCG group (treated by DMEM+H2O2 + EGCG).
RESULTS AND CONCLUSION: The apoptosis rate for LECs and the expression of Fas protein in H2O2 group were apparently higher than that in the blank control group (P < 0.05). Comparing with H2O2 group, apoptosis rate for LECs and the expression of Fas protein were significantly lower in EGCG group (P < 0.05). Results indicate that up-regulating the expression of Fas protein could be one of the mechanism through that H2O2 induced apoptosis of LECs. EGCG could down-regulate expression of Fas protein and reduce apoptosis of LECs.

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