MicroRNA expression profiles of chondrocytes in osteoarthritis induced by stromal cell derived factor 1

doi:10.3969/j.issn.2095-4344.2107

Abstract

Abstract:

BACKGROUND: Osteoarthritis is a complex disease caused by many factors, but its pathogenesis is yet unknown. Intensive molecular genetic research has indicated that noncoding RNA may be a potential transcriptional regulatory factor in osteoarthritis development. Exploration on differentially expressed miRNAs between osteoarthritis and normal tissue gives the clue to understand the molecular mechanism of osteoarthritis, providing a new cue for the diagnosis and treatment of osteoarthritis.

OBJECTIVE: To discuss the changes of miRNA expression profile of chondrocytes stimulated by stromal cell derived factor 1 in osteoarthritis, and provide an experimental basis for delaying articular cartilage degeneration at the genetic level.

METHODS: Residual cartilage samples from 10 patients with knee osteoarthritis who underwent total knee replacement were collected for culture of chondrocytes. The cells were then randomized into two groups: experimental group was cultured in high-glucose DMEM medium containing 10% fetal bovine serum and penicillin with the addition of 100 μg/L stromal cell derived factor 1, and control group was cultured in the medium with no other induction. At 48 hours after cell culture, the miRNA expression profiles of chondrocytes were detected, and the results were verified by fluorescence quantitative RT-PCR. The study protocol was performed in accordance with the Regulations on the Administration of Medical Institutions. All study patients gave written informed consent before collection of cartilage tissue.

RESULTS AND CONCLUSION: After preliminary screening, 84 microRNAs were altered, of which 70 were up-regulated and 14 were down-regulated. Seven microRNAs with significant changes (miR-146a-5p, miR-124-3p, miR-130a-3p, miR-185-5p, miR-221-3p, miR-126-3p) were selected by gene chip for qRT-PPCR experiment, which indicated that the qRT-PCR results of miR-146a-5p, miR-124-3p and miR-126-3p were consistent with the results of gene chip. Therefore, induction with stromal cell derived factor 1 makes the expression profile of circulating miRNA change a lot. miR-146a-5p, miR-124-3p and miR-126-3p may be related to the stromal cell derived factor 1/CXCR4 signaling pathway by which stromal cell derived factor 1 can stimulate osteoarthritis.

Key words: osteoarthritis, chondrocytes, SDF-1/CXCR4 signaling pathway, stromal cell derived factor 1, microRNA expression profiles

CLC Number:

Wang Guoliang, Li Yanlin, Xiang Yaoyu, Jia Di, Li Canzhang, He Lu. MicroRNA expression profiles of chondrocytes in osteoarthritis induced by stromal cell derived factor 1 [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(31): 4948-4953.

Figures/Tables 7

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