Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5201-5204.doi: 10.3969/j.issn.1673-8225.2011.28.018

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Infiltration of mast cells in renal interstitium in a rat model of adenine-induced chronic renal failure

Xing Hong-mei1, Yu Cai-yun2, Liu Xue-mei1, Liu Hong-yan1   

  1. 1Department of Nephrology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao   266003, Shandong Province, China
    2 Hospital of Qingdao Agricultural University, Qingdao 266109  Shandong Province, China
  • Received:2011-01-23 Revised:2011-02-28 Online:2011-07-09 Published:2011-07-09
  • Contact: Liu Xue-mei, Doctor, Associate chief physician, Department of Nephrology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China Liuxm2004@sina. com
  • About author:Xing Hong-mei★, Studying for master’s degree, Attending physician, Department of Nephrology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China plxhm@sina.com
  • Supported by:

    Scientific and Technological Project of Shandong Province, No. 2010G0020222*

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Abstract:

BACKGROUND: Recent studies have shown that infiltration of mast cells (MCs) is closely correlated with the degree of tubulointerstitial fibrosis in various renal diseases. Whether MCs are involved in renal interstitial fibrosis in a rat model of adenine-induced chronic renal failure is poorly understood.
OBJECTIVE: To investigate the distributing characteristics of MCs and the relationship between number of MCs in the interstitium and tubulointerstitial fibrosis.
METHODS: Forty-six male Wistar rats were randomly divided into control group and model group. The model group rats were intragastrically administered adenine at a dose of 150 mg/kg per day, and the control group rats were intragastrically administered the same amount of physiological saline. Blood and urine were collected for measuring creatinine and protein. Kidney sample sections were stained with hematoxylin and eosin, Masson’s trichrome. Renal interstitial fibrosis (RIF) was assessed by light microscope. The intensity and distribution of MCs were examined by toluidine blue and immunohistochemistry using tryptase antibody.
RESULTS AND CONCLUSION: Proteinuria and renal function of model rats deteriorated gradually with the elongation of intragastric administration time, and the score of RIF increased gradually. MCs were detected in the interstitium, periglomerular and perivascular areas in the model kidneys, in close proximity to areas of interstitial fibrosis, but were not found in the glomeruli. With aggravation of interstitial lesions degree, the number of MCs increased. There were significant differences in number of MCs, RIF score, urinary protein, serum creatinine and blood urea nitrogen between the model rats and control rats at different time points (P < 0.01). MCs were positively correlated with interstitial fibrosis (r = 0.96, P < 0.001). These findings suggest that increased infiltration of MCs is involved in renal interstitial fibrosis in a rat model of adenine-induced chronic renal failure, which provides an ideal animal model for the further research of MCs in renal tissue.

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