Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5205-5209.doi: 10.3969/j.issn.1673-8225.2011.28.019

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Tumor formation of gastrointestinal stromal tumor tissue in different parts of nude mice aged different weeks

Zhou Bin1, Hua Ya-wei1, Sheng Shu-juan2, Zhang Zhan-dong1, Wan Bai-shun1, Wang Zheng-wei1   

  1. 1Department of General Surgery of Cancer Hospital of Zhengzhou University, Department of General Surgery of Henan Tumor Hospital, Zhengzhou  450052, Henan Province, China
    2The Third People’s Hospital, Zhengzhou  450003, Henan Province, China
  • Received:2011-02-26 Revised:2011-05-11 Online:2011-07-09 Published:2011-07-09
  • Contact: Hua Ya-wei, Master, Chief physician, Department of General Surgery of Cancer Hospital of Zhengzhou University, Department of General Surgery of Henan Tumor Hospital, Zhengzhou 450052, Henan Province, China huayawei@yahoo.com.cn
  • About author:Zhou Bin★, Master, Physician, Department of General Surgery of Cancer Hospital of Zhengzhou University, Department of General Surgery of Henan Tumor Hospital, Zhengzhou 450052, Henan Province, China zhoubin610@yahoo.com.cn
  • Supported by:

    the Research Development Special Foundation of Henan Provincial Scientific Research Institute, No. 072103810502*

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Abstract:

BACKGROUND: Animal model of nude mouse of human gastrointestinal stromal tumor was established, which can provide a necessary tool for the study of gastrointestinal stromal tumor cells under living body.
OBJECTIVE: To observe tumor formation of the armpits and buttocks in different week-old nude mice after incubation of gastrointestinal stromal tumor cell tissue.
METHODS: The gastrointestinal stromal tumor tissue blocks were inoculated at 4-week-old nude mice under the armpits, 8-week-old nude mice under the armpits, 4-week-old nude mouse buttocks, 8-week-old nude mouse buttocks area to observe tumor formation rate, growth rate, ulceration rate and metastasis rate in a certain time, the different ages in different parts.
RESULTS AND CONCLUSION: Tumor formation rate and growth speed were significantly higher in the 4-week-old nude mice than in 8-week-old nude mice (P < 0.05). The tumor formation rate was greater in the armpits than in the buttocks. No significant difference in ulceration rate was determined between 4-week-old nude mice and 8-week-old nude mice (P > 0.05), but the ulceration rate was greater in the buttocks than in the armpits (P < 0.05). The metastasis rate was higher in 8-week-old nude mice than in 4-week-old nude mice (P < 0.05), and the metastasis rate was greater in the buttocks than in the armpits in the same-age nude mice. These indicate that regardless of how the vaccination site, in the smaller-week-old nude mice, the tumor formation rate was higher and tumor growth was faster; but for the same week-old nude mice, regardless of how the vaccination site, no significant differences in tumor growth rate were detected.

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