Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5193-5196.doi: 10.3969/j.issn.1673-8225.2011.28.016

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An effective rat model of severe acute pancreatitis

Hao Jian-zhi, Fang Chi-hua, Fan Ying-fang, Huang Yan-peng, Hu Hai-bei   

  1. First Department of Hepatobilliary Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-01-15 Revised:2011-02-10 Online:2011-07-09 Published:2011-07-09
  • Contact: Fang Chi-hua, Doctor, Chief physician, Professor, Doctoral supervisor, First Department of Hepatobilliary Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China fangch_dr@126.com
  • About author:Hao Jian-zhi★, Studying for master’s degree, Physician, First Department of Hepatobilliary Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China

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Abstract:

BACKGROUND: There is no animal model of severe acute pancreatitis that has the pathogenesis as same as human. Moreover, there are even fewer models used in experimental treatment studies.
OBJECTIVE: To construct an effective rat model of severe acute pancreatitis for the therapeutic study on severe acute pancreatitis rats.
METHODS: Thirty rats were randomly divided into control and model groups. The severe acute pancreatitis model was constructed by retrograde pancreatic duct infusion of Na-taurocholate. The expression of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α)  in the serum as well as amylase in the ascites and blood and white cell number in the blood were detected, and the pathological changes of pancreatic tissues were observed.
RESULTS AND CONCLUSION: The survival rate was 80% in the model group, and 100% in the control group. With increasing time, pathological score, IL-6 and TNF-α expression in the serum, white cell number and amylase in the ascites and blood were increased gradually (P < 0.05). It was indicated that the severe acute pancreatitis model was constructed successfully in rats.

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