中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (34): 6323-6327.doi: 10.3969/j.issn.1673-8225.2010.34.013

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

羟基磷灰石/聚乙烯醇水凝胶的制备与生物活性评价

吴  刚1，赵春华1，王成焘2，张文光2   

  1. 1三峡大学机械与材料学院，湖北省宜昌市  443002；2上海交通大学机械与动力工程学院，上海市  200030
  • 出版日期:2010-08-20 发布日期:2010-08-20
  • 作者简介:吴刚☆, 男, 1978年生, 湖北省汉川市人，汉族，2007年上海交通大学毕业，博士, 主要从事生物材料、生物摩擦学方面的研究。 wwwg2000@sina.com.cn
  • 基金资助:

    国家自然科学基金项目资助 (50905097/ E050504)；湖北省教育厅基金项目资助 (Q20091306)。

Preparation and bioactivity of nano-hydroxyapatite/polyvinyl alcohol-hydrogel composite

Wu Gang1, Zhao Chun-hua1, Wang Cheng-tao2, Zhang Wen-guang2   

  1. 1 College of Mechanical and Material Engineering, China Three Gorges University, Yichang   443002, Hubei Province, China; 2 School of Mechanical and Power Engineering, Shanghai Jiao Tong University, Shanghai   200030, China
  • Online:2010-08-20 Published:2010-08-20
  • About author:Wu Gang☆, Doctor, College of Mechanical and Material Engineering, China Three Gorges University, Yichang 443002, Hubei Province, China wwwg2000@sina.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No. 50905097/ E050504*; a grant by Hubei Provincial Education Ministry, No. Q20091306*

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592

被引次数

16

摘要：

背景：先前的研究表明，羟基磷灰石/聚乙烯醇水凝胶复合水凝胶(hydroxyapatite/ polyvinyl alcohol hydrogel,HA/PVA-H)具有较好的机械性能和耐摩擦磨损性能，这说明HA/PVA-H复合材料具有早期承载能力和较好的生物活性。那么HA/PVA-H复合材料能否通过羟基磷灰石粒子与周围骨组织较快地形成活性结合呢？
目的：制备羟基磷灰石/聚乙烯醇水凝胶，并对其进行生物学评价。
方法：将Ca(OH)2研磨过筛后，配制成一定浓度的悬浮分散液；加入质量分数15%的PVA水溶液中，添加二甲亚砜，最后按Ca/P比1.67: 1加入H3PO4的乙醇溶液，制备HA/PVA-H。对试样进行体外模拟体液培养实验，测试浸泡前后SBF浸泡液的变化，并利用扫描电镜、FTIR、XRD 对材料的结构进行了表征和分析。
结果与结论：扫描电镜观察可知羟基磷灰石/聚乙烯醇水凝胶表面有结晶体形成，经XRD分析确认为弱结晶的羟基磷灰石晶体；浸泡之后浸泡液的pH值与Ca、P离子浓度下降，同时FTIR结果显示试样中的PO4 3-特征峰得到增强，且有CO32-的特征峰形成。结果提示，羟基磷灰石/聚乙烯醇水凝胶复合材料具有较好的生物活性。

关键词: 聚乙烯醇水凝胶, 羟基磷灰石, 人工软骨材料, 模拟体液, 生物活性

Abstract:

BACKGROUND: Previous studies have shown that, nano-hydroxyapatite/polyvinyl alcohol hydrogel (nano-HA/PVA-H) composite exhibits good mechanical property, friction and ware resistance, indicating early load capacity and good bioactivity of HA/PVA-H composite. Does HA/PVA-H composite material form binding activity with surrounding bone tissues through HA particles?
OBJECTIVE: To prepare the nano-HA/PVA-H composite and to evaluate the biological property of the material.
METHODS: After Ca(OH)2 grinding, suspended dispersion solution was made at a certain concentration; adding an appropriate amount of 15% mass fraction PVA aqueous solution, then dimethyl sulfoxide, finally H3PO4 ethanol solution at Ca/P ratio of 1.67: 1. The HA/PVA-H composite was prepared and processed into in vitro simulated body fluid test. Changes of simulated body fluid solution before and after soaking process were investigated. Scanning electron microscopy, FTIR and XRD were used to characterize the structure and component of composite materials.
RESULTS: Scanning electron microscopy showed that, nano-size crystals were formed on the surface of HA/PVA-H after soaking. XRD analysis proved that these crystals were HA particles. The pH value of simulated body fluid solution, and concentrations of Ca and P ions were decreased after soaking. Characteristic peaks of PO4 3- were enhanced and new peaks of CO3 2- were found after soaking on the FTIR experiments. Results showed that HA/PVA-H composite materials have good bioactivities.

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