中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (32): 6044-6047.doi: 10.3969/j.issn.1673-8225.2010.32.034

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

人端粒酶反转录酶基因转染内皮祖细胞移植治疗大鼠心肌梗死

李 娟，张树林   

  1. 解放军北京军区总医院干四科， 北京市  100125
  • 出版日期:2010-08-06 发布日期:2010-08-06
  • 作者简介:李娟★，女，1978年生，甘肃省武山市人，汉族，2008年解放军第四军医大学毕业，硕士，医师，主要从事动脉粥样硬化研究。zhenxi05@sohu.com

Transplantation of endothelial progenitor cells transfected with human telomerase reverse transcriptase gene for treatment of rat myocardial infarction 

Li Juan, Zhang Shu-lin   

  1. Forth Department of Senior Cadre, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing  100125, China
  • Online:2010-08-06 Published:2010-08-06
  • About author:Li Juan★, Master, Physician, Forth Department of Senior Cadre, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing 100125, China zhenxi05@sohu.com

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摘要：

背景：大量动物实验证明内皮祖细胞移植确实能改善梗死区供血，增加侧支循环。但其在外周血中含量较少，扩增后细胞数量也非常有限。
目的：观察人端粒酶反转录酶基因转染内皮祖细胞后该细胞的增殖分化情况及其对心肌梗死区血管新生及心功能的影响。
方法：以“人端粒酶反转录酶，内皮祖细胞，心肌梗死，转染，种子细胞”为中文关键词，“endothelial progenitor cells（EPCs），human telomerase reverse transcriptase(HTERT)，myocardial infarction ,seed cells ”为英文关键词，采用计算机检索1993-01/2010-04 PubMed数据库、中国期刊全文数据库相关文章。以30篇文献为主重点讨论人端粒酶反转录酶转染内皮祖细胞在心肌梗死中的作用。
结果与结论：人端粒酶反转录酶能够成功转染内皮祖细胞，利用人端粒酶反转录酶可提高增殖能力、延长细胞寿命的特性优化内皮祖细胞的活性，增加内皮祖细胞修复内膜、血管新生的作用，以达到治疗心肌梗死的目的。目前国内利用人端粒酶反转录酶转染内皮祖细胞治疗心肌梗死的实验研究尚未见报道。人端粒酶反转录酶转染内皮祖细胞后可提高该细胞的增殖能力，增加内皮祖细胞的活性，促进心肌梗死后梗死区心肌血管新生，改善心功能。

关键词: 端粒酶反转录酶, 内皮祖细胞, 心肌梗死, 转染, 种子细胞

Abstract:

BACKGROUND: Many animal experiments showed that endothelial progenitor cells (EPCs) could ameliorate myocardial ischemia and increase collateral circulation after myocardial infarction. However, EPCs are less in the peripheral blood and the contents of the amplified cells are also very limited.
OBJECTIVE: To observe proliferation and differentiation of EPCs transfected with telomerase reverse transcriptase gene, and effects of EPC transplantation on neovascularization and cardiac function in the region of myocardial infarction.
METHODS: A computer-based search was performed in PubMed and Chinese Journal Full-text Database for relevant articles published from January 1993 to April 2010. The key words were “endothelial progenitor cells, human telomerase reverse transcriptase, myocardial infarction, seed cells”. To focus on thirty documents, we discussed the effect of EPCs transfected with telomerase reverse transcriptase gene in treatment of myocardial infarction. 
RESULTS: Human telomerase reverse transcriptase gene could be successfully transfected into EPCs, and EPCs transfected with human telomerase reverse transcriptase gene could enhance the abilities of proliferation, prolong life, and improve the abilities of neovascularization and endomembrane repair in order to treat myocardial infarction. There is no report concerning EPCs transfected with human telomerase reverse transcriptase for treatment of myocardial infarction.
CONCLUSION: The EPCs modified by human telomerase reverse transcriptase gene could enhance the abilities of proliferation, and increase activity of EPCs, improve neovascularization and improve cardiac function after myocardial infarction.

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