中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (30): 5622-5625.doi: 10.3969/j.issn.1673-8225.2010.30.027

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

超声治疗应力屏蔽大鼠跟腱白细胞介素1β的表达

张  晶，马燕红，王  伟，晏小燕，赵华坤   

  1. 上海交通大学附属第六人民医院康复医学科，上海市  200232
  • 出版日期:2010-07-23 发布日期:2010-07-23
  • 通讯作者: 马燕红，副主任医师，上海交通大学附属第六人民医院康复医学科，上海市 200232
  • 作者简介:张晶★，男，1983年生，陕西省榆林市绥德县人，上海交通大学医学院在读硕士，主要从事骨关节康复方面的研究。 d-zj@yeah.net

Interleukin 1 beta expression in Achilles tendon of stress-shielded rats following ultrasound treatment  

Zhang Jing, Ma Yan-hong, Wang Wei, Yan Xiao-yan, Zhao Hua-kun   

  1. Department of Rehabilitation Medicine，Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University，Shanghai   200233，China 
  • Online:2010-07-23 Published:2010-07-23
  • Contact: Ma Yan-hong, Associate chief physician, Department of Rehabilitation Medicine，Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China d-zj@yeah.net
  • About author:Zhang Jing★, Studying for master’s degree, Department of Rehabilitation Medicine，Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233，China d-zj@yeah.net

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摘要：

背景：临床上超声被用于软组织急慢性损伤的治疗，但是关于超声波治疗肌腱挛缩的实验研究较少。
目的：观察超声对应力屏蔽后大鼠跟腱的作用和白细胞介素1β水平的影响。
方法：将24只SD大鼠随机分为正常组，造模组和超声治疗组，后2组大鼠左后肢用跟腱环扎联合坐骨神经切断的方法造模，治疗组术后3周开始进行超声治疗，强度为1 W/cm2，频率为1 MHz，通胀比为20%，每周治疗5 d，1次/d，10 min/次，治疗共3周。术后6周取其左后肢部分跟腱组织，进行透射电镜检测，并采用ELISA法检测白细胞介素1β水平。
结果与结论：超声治疗3周后胶原纤维排列较造模组整齐，细小纤维减少，接近于正常对照组。造模组白细胞介素1β水平显著高于正常组，超声治疗后白细胞介素1β水平显著降低。结果提示超声治疗可以对挛缩跟腱有治疗作用，可能与降低挛缩跟腱中白细胞介素1β水平有关。

关键词: 跟腱, 超声治疗, 应力屏蔽, 白细胞介素1&beta, 医学影像

Abstract:

BACKGROUND: Ultrasound has been clinically used to treat acute and chronic soft tissue injury, but the ultrasonic treatment for tendon contracture has been rarely reported.
OBJECTIVE: To observe the effect of ultrasonic treatment on level of interleukin 1β (IL-1β) in Achilles of stress-shielded rats．
METHODS: Twenty-four SD rats were randomly divided into three groups: normal group, model group, and ultrasound treatment group. The left hind limbs of all rats of the later two groups were modeled using Achilles tendon cerclage combined with sciatic nerve cut method. At 3 weeks after surgical operation, the treatment group was subjected to ultrasound treatment, strength 1W/cm2, frequency 1MHz, inflation ratio 20%, 5 days per week, once per day, 10 minutes once, for a total of 3 weeks. At 6 weeks post-surgery, the left hind limb Achilles tendon tissue was detected by transmission electron microscopy and the level of IL-1β was detected by enzyme-labeled immunosorbent assay. 
RESULTS AND CONCLUSION: After 3 weeks of ultrasound treatment, collagen fibers arranged neater than in the model, small fibers decreased, closed to the control group. The level of IL-1β in the model group was significantly higher than the normal group. After ultrasound treatment, the level of IL-1β was dramatically decreased. The results suggested that ultrasound treatment played an effective role in the treatment of Achilles tendon contracture, which might reduce the level of IL-1β in the Achilles tendon.

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