中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4415-4418.doi: 10.3969/j.issn.1673-8225.2011.24.011

• 骨组织构建 bone tissue construction • 上一篇    下一篇

自体富含血小板血浆对兔骨折愈合的影响

周海洋，王  宸，耿  震   

  1. 东南大学附属中大医院骨科，江苏省南京市，210009
  • 收稿日期:2011-02-09 修回日期:2011-05-12 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 王宸，教授，硕士生导师，东南大学附属中大医院骨科，江苏省南京市210009 wangchen@medmail.com.cn
  • 作者简介:周海洋★，1979年生，山东省苍山县人，汉族，东南大学在读硕士，主要从事骨与关节损伤的研究。 haiyangzhou 2008@163.com

Role of autologous platelet-rich plasma on fracture healing in rabbits

Zhou Hai-yang, Wang Chen, Geng Zhen   

  1. Department of Orthopedics, the Affiliated Zhongda Hospital of Southeast University, Nanjing  210009, Jiangsu Province, China
  • Received:2011-02-09 Revised:2011-05-12 Online:2011-06-11 Published:2011-06-11
  • Contact: Wang Chen, Professor, Master’s supervisor, Department of Orthopedics, the Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China wangchen@medmail.com.cn
  • About author:Zhou Hai-yang★, Studying for master’s degree, Department of Orthopedics, the Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China haiyangzhou2008@163.com

PDF

587

被引次数

6

摘要：

背景：研究表明富含血小板血浆常通过同源异体制备，可应用于促进扁骨和松质骨的骨折愈合。
目的：用Landsbergs法制备自体富含血小板血浆，观察其对兔桡骨骨折愈合影响。
方法：将新西兰兔随机分成富含血小板血浆组和对照组，分别建立桡骨中下1/3处骨折模型，富含血小板血浆组中加入制备的自体富含血小板血浆凝胶，对照组建立骨折模型后直接缝合。采用Landsbergs法制备自体富含血小板血浆，并植入富含血小板血浆组的骨折端，并行骨折断端石膏外固定。
结果与结论：兔骨折标本在建模后1，2，4，6周均有不同程度骨痂形成。富含血小板血浆组在建模后第4周X射线进行影像学评分明显高于对照组(P < 0.05)。免疫组织化学检测显示：富含血小板血浆组Ⅰ型胶原量明显的高于对照组(P < 0.05)，可持续4周左右。结果证实，富含血小板血浆可通过增加骨痂中骨岛量和加速Ⅰ型胶原合成而明显促进长骨骨折愈合。

关键词: 富含血小板血浆, 长骨, 骨折愈合, 成骨细胞, 血小板源性细胞因子, Ⅰ型胶原, 纤维蛋白, 组织构建

Abstract:

BACKGROUND: Platelet-rich plasma (PRP) is often prepared with autalloploid and can be used to promote the fracture healing of flat bone and cancellous bone.
OBJECTIVE: To prepare PRP with Landsberger’s method and to analyzes the therapeutic role of PRP on the fracture healing of radius in rabbits.
METHODS: New Zealand rabbits were randomly divided into a control group and a PRP treated group. Unilateral open femoral fracture models were established in the lower 1/3 radius bone. PRP group received was treated with autologous PRP gel, while control group was only sutured after fracture modeling. Autologous PRP was prepared by Landsberger’s method and then implanted to the fracture site, followed by plaster immobilization of the fracture fragments.
RESULTS AND CONCLUSION: The fracture specimens exhibited varying degrees of callus formation at 1, 2, 4, 6 weeks. X-ray score in the PRP group was significantly higher than that in control group at 4 weeks (P < 0.05). Immunohistochemistry showed that, type I collagen content in the PRP treatment group was significantly higher than that in the control groups for 4 weeks (P < 0.05). PRP accelerates bone fracture healing of rat femurs by increasing expression of type I collagen and the number of bone island.

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